AB202819

Recombinant rat FGF21 protein

Bioactive
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Recombinant rat FGF21 protein is a Full Length protein, in the 29 to 208 aa range, expressed in Escherichia coli, with >95%, > 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, HPLC.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

> 1 EU/µg

Expression system

Escherichia coli

Applications

FuncS, HPLC, SDS-PAGE

applications

Biologically active

Yes

Biological activity

ab202819 is fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/mL, corresponding to a specific activity of > 2.0 × 10³ IU/mg in the presence of 5 μg/mL of rMuKlotho-β and 10 μg/mL of heparin.

Animal free

Carrier free

Yes

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions. Reconstituted protein should be stored in working aliquots and stored at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Storage buffer

pH: 7.4 Constituents: PBS, 2.9% Sodium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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Sequence info

[{"sequence":"AYPISDSSPLLQFGGQVRQRYLYTDDDQDTEAHLEIREDGTVVGTAHRSPESLLELKALKPGVIQILGVKASRFLCQQPDGTLYGSPHFDPEACSFRELLLKDGYNVYQSEAHGLPLRLPQKDSQDPATRGPVRFLPMPGLPHEPQEQPGVLPPEPPDVGSSDPLSMVEPLQGRSPSYAS","proteinLength":"Full Length","predictedMolecularWeight":"19.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":208,"aminoAcidStart":29,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":null,"tags":[]}]

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

True

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGF21 or fibroblast growth factor 21 is an important metabolic regulator. It is a member of the fibroblast growth factor family but unlike many others FGF21 functions mainly in endocrine signaling. The molecular weight of FGF21 is approximately 22 kDa. This protein is expressed in tissues such as the liver adipose tissue and pancreas. Because of its endocrine roles FGF21 travels through the bloodstream to exert its functions on distant organs.

Biological function summary

FGF21 influences glucose and lipid metabolism. It does not act as part of a larger protein complex. Instead it directly affects metabolic processes enhancing insulin sensitivity and lipid oxidation while reducing body weight. These actions help maintain energy balance and homeostasis making FGF21 an important player in metabolic control.

Pathways

FGF21 plays a significant role in the insulin signaling pathway and PPARγ coactivator 1α (PGC-1α) pathway. These pathways regulate cellular energy metabolism and mitochondrial biogenesis respectively. FGF21 interacts with other molecules such as PPARα (peroxisome proliferator-activated receptor alpha) to exert its effects demonstrating its integration into broader metabolic networks. FGF21's interaction with these pathways highlights its function in energy utilization and storage.

FGF21 has connections to conditions such as obesity and type 2 diabetes. Its role in improving insulin sensitivity and promoting lipid oxidation makes it a molecule of interest in combating metabolic syndrome. FGF21 also has a relationship with hepatic proteins such as insulin receptor substrates (IRS) which play roles in liver functioning. Understanding these relationships offers insights into potential therapeutic strategies targeting metabolic disorders.

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Specifications

Form

Lyophilized

Additional notes

Purity is determined by SDS-PAGE and HPLC analyses.

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General info

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Fgf21

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Recombinant rat FGF21 protein

Key facts

Purity

Endotoxin level

Expression system

Tags

Applications

Biologically active

Biological activity

Animal free

Carrier free

Reconstitution

Storage buffer

Reactivity data

Sequence info

Properties and storage information

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Specifications

Form

Additional notes

General info

Product protocols

Target data

Complementary Products

Secondary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Product promise