Recombinant rat FGF9/GAF protein is a Rat Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
Amino acid sequence
P L G E V G S Y F G V Q D A V P F G N V P V L P V D S P V L L N D H L G Q S E A G G L P R G P A V T D L D H L K G I L R R R Q L Y C R T G F H L E I F P N G T I Q G T R K D H S R F G I L E F I S I A V G L V S I R G V D S G L Y L G M N E K G E L Y G S E K L T Q E C V F R E Q F E E N W Y N T Y S S N L Y K H V D T G R R Y Y V A L N K D G T P R E G T R T K R H Q K F T H F L P R P V D P D K V P E L Y K D I L S Q S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
Alternative names
Fibroblast growth factor 9, FGF-9, Glia-activating factor, HBGF-9, GAF, Fgf-9, Fgf9
Recommended products
Recombinant rat FGF9/GAF protein is a Rat Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
ab78476 is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors, is <0.5 ng/ml.
- Accession
- P36364-1
- Animal free
No
- Species
Rat
- Reconstitution
Reconstitute at 0.1 mg/mL in water
- Concentration
- Storage buffer
Constituents: 0.99% Ammonium sulphate, 0.16% Sodium phosphate
Sequence info
Amino acid sequence
P L G E V G S Y F G V Q D A V P F G N V P V L P V D S P V L L N D H L G Q S E A G G L P R G P A V T D L D H L K G I L R R R Q L Y C R T G F H L E I F P N G T I Q G T R K D H S R F G I L E F I S I A V G L V S I R G V D S G L Y L G M N E K G E L Y G S E K L T Q E C V F R E Q F E E N W Y N T Y S S N L Y K H V D T G R R Y Y V A L N K D G T P R E G T R T K R H Q K F T H F L P R P V D P D K V P E L Y K D I L S Q S
- Accession
- Protein length
Full Length
- Nature
Recombinant
Specifications
- Form
Lyophilized
- Additional notes
Purity is approximately 95% as determined by reducing and non-reducing SDS-PAGE.Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
General info
- Function
Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
- Sequence similarities
Belongs to the heparin-binding growth factors family.
- Post-translational modifications
N-glycosylated.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
Stable for 12 months at -20°C
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Previously labelled as FGF9.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FGF9 also known as GAF is a member of the fibroblast growth factor family. The FGF9 protein has a molecular mass of approximately 23 kDa. It is commonly expressed in the brain kidney and lungs during fetal development. The FGF9 protein functions as a signaling molecule influencing cell proliferation and differentiation which are essential for proper tissue formation and organogenesis.
- Biological function summary
FGF9 contributes to important processes in the development and maintenance of several tissues. It is not known to be part of a larger complex. FGF9 regulates cellular activities such as proliferation and differentiation through its interaction with specific receptors on the cell surface. Its role is significant in skeletal and nervous system development where it ensures proper maturation and function of these systems.
- Pathways
The FGF9 protein participates in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are important for cell survival proliferation and differentiation. FGF9 interacts closely with other fibroblast growth factors such as FGF2 to ensure effective signaling cascades. These interactions affect various cellular responses and contribute to the normal development of multiple organ systems.
- Associated diseases and disorders
Alterations in FGF9 expression can be linked to conditions such as lung fibrosis and cancer. Aberrant FGF9 signaling can contribute to uncontrolled cell proliferation and fibrosis formation. The GAF protein's dysregulation may involve interactions with receptor proteins including FGFR2 in these pathological processes. Understanding FGF9's role in these diseases helps in developing targeted therapeutic strategies.
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2 product images
SDS-PAGE - Recombinant rat FGF9/GAF protein (ab78476)
SDS PAGE analysis of ab78476 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.
Functional Studies - Recombinant rat FGF9/GAF protein (ab78476)
ab78476 used in Functional Studies.
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