JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB281795

Recombinant rat GDNF protein (Active)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant rat GDNF protein (Active) is a Rat Full Length protein, in the 78 to 211 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC.

View Alternative Names

Glial cell line-derived neurotrophic factor, Astrocyte-derived trophic factor, ATF, Gdnf

4 Images
Mass Spectrometry - Recombinant rat GDNF protein (Active) (AB281795)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant rat GDNF protein (Active) (AB281795)

Mass determination by ESI-TOF.

Predicted MW is 14988.03 Da (+/- 10 da by ESI-TOF). Observed MW is 14989.70 Da.

Functional Studies - Recombinant rat GDNF protein (Active) (AB281795)
  • FuncS

Supplier Data

Functional Studies - Recombinant rat GDNF protein (Active) (AB281795)

Functional analysis of ab281795.

Fully biologically active determined by the dose dependent proliferation of SH-SY5Y cells. ED50 is ≤40.7 ng/ml, corresponding to a specific activity of 2.5 x 104 unit/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot : GR3391461-1

SDS-PAGE - Recombinant rat GDNF protein (Active) (AB281795)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant rat GDNF protein (Active) (AB281795)

SDS-PAGE analysis of ab281795.

HPLC - Recombinant rat GDNF protein (Active) (AB281795)
  • HPLC

Supplier Data

HPLC - Recombinant rat GDNF protein (Active) (AB281795)

HPLC analysis of ab281795.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

FuncS, HPLC, SDS-PAGE, Mass Spec

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by the dose dependent proliferation of SH-SY5Y cells. ED50 is ≤40.7 ng/ml, corresponding to a specific activity of 2.5 x 104 unit/mg.

Accession

Q07731

Animal free

Yes

Carrier free

No

Species

Rat

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"SPDKQAAALPRRERNRQAAAASPENSRGKGRRGQRGKNRGCVLTAIHLNVTDLGLGYETKEELIFRYCSGSCEAAETMYDKILKNLSRSRRLTSDKVGQACCRPVAFDDDLSFLDDSLVYHILRKHSAKRCGCI","proteinLength":"Full Length","predictedMolecularWeight":"14.99 kDa","actualMolecularWeight":"14.99 kDa","aminoAcidEnd":211,"aminoAcidStart":78,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q07731","tags":[]}]
style
left-column

Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
style
left-column
style
left-column

Specifications

Form

Lyophilized

Additional notes

>=95% purity by HPLC.

style
left-column

General info

Function

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake (PubMed : 10828539). Acts by binding to its coreceptor, GFRA1, leading to autophosphorylation and activation of the RET receptor (By similarity). May also modulate local neuronal effects in distal regions of the motor neuron (PubMed : 10828539). Involved in the development of the neural crest (By similarity).

Sequence similarities

Belongs to the TGF-beta family. GDNF subfamily.

style
left-column

Product protocols

style
left-column

Target data

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake (PubMed : 10828539). Acts by binding to its coreceptor, GFRA1, leading to autophosphorylation and activation of the RET receptor (By similarity). May also modulate local neuronal effects in distal regions of the motor neuron (PubMed : 10828539). Involved in the development of the neural crest (By similarity).
See full target information Gdnf
style
left-column
style
left-column
style
right-column

Complementary Products

Proteins & Peptides

AB206642

Recombinant human BDNF protein

Bioactive

Functional Studies - Recombinant human BDNF protein (AB206642)

  • 0
  • 0
Proteins & Peptides

AB280343

Recombinant Mouse IL-21 protein (Active)

Premium Bioactive

Functional Studies - Recombinant Mouse IL-21 protein (Active) (AB280343)

  • 0
  • 0
Cell Lines & Lysates

AB166833

Hep G2 whole cell lysate

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com