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AB198570

Recombinant rat IGF1 protein

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(3 Publications)

Recombinant rat IGF1 protein is a Rat Full Length protein, in the 49 to 118 aa range, expressed in Escherichia coli, with >95%, >1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, HPLC.

View Alternative Names

Igf-1, Igf1, Insulin-like growth factor 1, Insulin-like growth factor I, Somatomedin, IGF-I

1 Images
SDS-PAGE - Recombinant rat IGF1 protein (AB198570)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant rat IGF1 protein (AB198570)

SDS PAGE analysis of ab198570 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

>1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

HPLC, SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The activity is measured in a proliferation assay using FDC-P1 cells and is typically less than 100 ng/mL.

Accession

P08025

Animal free

No

Carrier free

No

Species

Rat

Reconstitution

Reconstitute at 0.1 mg/mL in water

Storage buffer

Constituents: 0.1% Trifluoroacetic acid

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Sequence info

[{"sequence":"GPETLCGAELVDALQFVCGPRGFYFNKPTGYGSSIRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPTKSA","proteinLength":"Full Length","predictedMolecularWeight":"7.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":118,"aminoAcidStart":49,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P08025","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The insulin-like growth factor 1 or IGF1 is a protein hormone that plays an important mechanical role in growth and development. Alternative names for IGF1 include somatomedin C. This protein has a mass of approximately 7.6 kDa and is mainly produced by the liver. IGF1 is expressed in a range of tissues including muscle bone and cartilage reflecting its importance in various physiological processes. Its production is stimulated by growth hormone and it acts in an autocrine and paracrine manner to exert its effects.
Biological function summary

IGF1 stimulates growth and proliferation of cells most notably impacting the development of the skeletal system and the regulation of apoptosis. It is not part of a larger protein complex but it interacts closely with IGF1 receptor to execute its functions. By binding to this receptor IGF1 activates intracellular signaling cascades that promote anabolic effects which include increased uptake of glucose and amino acids contributing to muscle and tissue growth.

Pathways

IGF1 operates within the PI3K-Akt and MAPK signaling pathways which are significant for cell survival and proliferation. Its interaction with proteins like IGF binding protein 3 (IGFBP3) modulates its availability and activity in the bloodstream. These pathways are critical for mediating the effects of growth hormone highlighting the role of IGF1 in systemic growth and metabolism regulation.

Alterations in IGF1 levels associate with growth abnormalities such as Laron syndrome and acromegaly. Laron syndrome results from the body's inability to use IGF1 properly due to receptor defects while acromegaly occurs from excessive IGF1 production often due to pituitary tumors. Furthermore aberrant IGF1 signaling connects to cancer development where it can drive tumor growth and metastasis. Drugs targeting IGF1 and its pathway components continue to be researched for therapeutic potential in these conditions.
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Specifications

Form

Lyophilized

Additional notes

ab198570 is greater or equal to 98% pure as assessed by HPLC and SDS-PAGE.

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General info

Function

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV : ITGB3 and ITGA6 : ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (By similarity). As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM (PubMed : 20724525).

Sequence similarities

Belongs to the insulin family.

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Product protocols

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Target data

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV : ITGB3 and ITGA6 : ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (By similarity). As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM (PubMed : 20724525).
See full target information Igf1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Microbiology spectrum 12:e0283823 PubMed38197658

2024

Application of bacteriophage φPaP11-13 attenuates rat infection lesions by promoting keratinocytes apoptosis via inhibiting PI3K/Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Liu,Ni Zhen,Danxi Liao,Jiahui Niu,Ruolan Liu,Zijiao Li,Zeyuan Lei,Zichen Yang

APL bioengineering 3:036106 PubMed31489396

2019

Automated sensing and splitting of stem cell colonies on microraft arrays.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew DiSalvo,Nicole M Smiddy,Nancy L Allbritton

PloS one 11:e0167222 PubMed27893850

2016

A Convenient In Vivo Model Using Small Interfering RNA Silencing to Rapidly Assess Skeletal Gene Function.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Zhang,Can Liu,Bao Hai,Guohong Du,Hong Wang,Huijie Leng,Yingsheng Xu,Chunli Song
View all publications
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We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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