Recombinant rat IL-1 beta protein (Active) is a Rat Fragment protein, in the 117 to 268 aa range, expressed in HEK 293, with >95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC.
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Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, MS, HPLC
- Biologically active
Yes
Amino acid sequence
V P I R Q L H C R L R D E Q Q K C L V L S D P C E L K A L H L N G Q N I S Q Q V V F S M S F V Q G E T S N D K I P V A L G L K G L N L Y L S C V M K D G T P T L Q L E S V D P K Q Y P K K K M E K R F V F N K I E V K T K V E F E S A Q F P N W Y I S T S Q A E H R P V F L G N S N G R D I V D F T M E P V S S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
Alternative names
Interleukin-1 beta, IL-1 beta, Il1b
Recommended products
Recombinant rat IL-1 beta protein (Active) is a Rat Fragment protein, in the 117 to 268 aa range, expressed in HEK 293, with >95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC.
Alternative names
Interleukin-1 beta, IL-1 beta, Il1b
Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, MS, HPLC
- Biologically active
Yes
- Biological activity
Fully biologically active determined by the dose dependent proliferation of D10.G4.1 cells.
ED50 is ≤0.18 ng/ml, corresponding to a specific activity of 5.6 x 106 units/mg.
- Accession
- Q63264-1
- Animal free
Yes
- Species
Rat
- Reconstitution
Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
V P I R Q L H C R L R D E Q Q K C L V L S D P C E L K A L H L N G Q N I S Q Q V V F S M S F V Q G E T S N D K I P V A L G L K G L N L Y L S C V M K D G T P T L Q L E S V D P K Q Y P K K K M E K R F V F N K I E V K T K V E F E S A Q F P N W Y I S T S Q A E H R P V F L G N S N G R D I V D F T M E P V S S
- Accession
- Protein length
Fragment
- Predicted molecular weight
17 kDa
- Actual molecular weight
17.36 kDa
- Amino acids
117 to 268
- Nature
Recombinant
Specifications
- Form
Lyophilized
- Additional notes
>95% by HPLC
General info
- Function
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
- Sequence similarities
Belongs to the IL-1 family.
- Subcellular localisation
Lysosome
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Functional Studies - Recombinant rat IL-1 beta protein (Active) (ab281807)
Functional analysis of ab281807.
Fully biologically active determined by the dose dependent proliferation of D10.G4.1 cells.
ED50 is ≤0.18 ng/ml, corresponding to a specific activity of 5.6 x 106 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3384878-2
SDS-PAGE - Recombinant rat IL-1 beta protein (Active) (ab281807)
SDS-PAGE analysis of ab281807.
Mass Spectrometry - Recombinant rat IL-1 beta protein (Active) (ab281807)
Mass determination by ESI-TOF.
Predicted MW is 17359.98 Da. (+/- 10 Da by ESI-TOF). Observes MW is 17362.38 Da.
HPLC - Recombinant rat IL-1 beta protein (Active) (ab281807)
HPLC analysis of ab281807.
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