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AB281807

Recombinant rat IL-1 beta protein (Active)

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Recombinant rat IL-1 beta protein (Active) is a Rat Fragment protein, in the 117 to 268 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC.

View Alternative Names

Interleukin-1 beta, IL-1 beta, Il1b

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Mass Spectrometry - Recombinant rat IL-1 beta protein (Active) (AB281807)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant rat IL-1 beta protein (Active) (AB281807)

Mass determination by ESI-TOF.

Predicted MW is 17359.98 Da. (+/- 10 Da by ESI-TOF). Observes MW is 17362.38 Da.

Functional Studies - Recombinant rat IL-1 beta protein (Active) (AB281807)
  • FuncS

Supplier Data

Functional Studies - Recombinant rat IL-1 beta protein (Active) (AB281807)

Functional analysis of ab281807.

Fully biologically active determined by the dose dependent proliferation of D10.G4.1 cells.

ED50 is ≤0.18 ng/ml, corresponding to a specific activity of 5.6 x 106 units/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot : GR3384878-2

HPLC - Recombinant rat IL-1 beta protein (Active) (AB281807)
  • HPLC

Supplier Data

HPLC - Recombinant rat IL-1 beta protein (Active) (AB281807)

HPLC analysis of ab281807.

SDS-PAGE - Recombinant rat IL-1 beta protein (Active) (AB281807)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant rat IL-1 beta protein (Active) (AB281807)

SDS-PAGE analysis of ab281807.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

FuncS, SDS-PAGE, HPLC, Mass Spec

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by the dose dependent proliferation of D10.G4.1 cells.

ED50 is ≤0.18 ng/ml, corresponding to a specific activity of 5.6 x 106 units/mg.

Accession

Q63264

Animal free

Yes

Carrier free

No

Species

Rat

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Primary Antibodies

AB283818

Anti-IL-1 beta antibody [RM1009]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"VPIRQLHCRLRDEQQKCLVLSDPCELKALHLNGQNISQQVVFSMSFVQGETSNDKIPVALGLKGLNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVFNKIEVKTKVEFESAQFPNWYISTSQAEHRPVFLGNSNGRDIVDFTMEPVSS","proteinLength":"Fragment","predictedMolecularWeight":"17 kDa","actualMolecularWeight":"17.36 kDa","aminoAcidEnd":268,"aminoAcidStart":117,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q63264","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Specifications

Form

Lyophilized

Additional notes

>95% by HPLC

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General info

Function

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.

Sequence similarities

Belongs to the IL-1 family.

Subcellular localisation

Lysosome

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Product protocols

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Target data

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
See full target information Il1b
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Product promise

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