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AB233668

Recombinant rat IL-3 protein (Active)

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Recombinant rat IL-3 protein (Active) is a Rat Full Length protein, in the 27 to 166 aa range, expressed in Escherichia coli, with >95%, <1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

Il-3, Il3, Interleukin-3, IL-3, Hematopoietic growth factor, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor, MCGF

2 Images
Functional Studies - Recombinant rat IL-3 protein (Active) (AB233668)
  • FuncS

Supplier Data

Functional Studies - Recombinant rat IL-3 protein (Active) (AB233668)

ab233668 induced proliferation of NFS-60 cells.

SDS-PAGE - Recombinant rat IL-3 protein (Active) (AB233668)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant rat IL-3 protein (Active) (AB233668)

1 μg ab233668 analyzed on a 4-20% Tris-Glycine gel, stained with Coomassie Blue.

Lane 1 : Non-reducing conditions.

Lane 2 : Reducing conditions.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

NFS-60 cell proliferation: ED50 ≤ 10 ng/ml (≥ 1.0 x 104 units/mg).

Accession

P04823

Animal free

No

Carrier free

No

Species

Rat

Reconstitution

Reconstitute at 0.1 mg/mL in water

Storage buffer

Constituents: 0.1% Trifluoroacetic acid

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"MISDRGSDAHHLLRTLDCRTIALEILVKLPYPQVSGLNNSDDKANLRNSTLRRVNLDEFLKSQEEFDSQDTTDIKSKLQKLKCCIPAAASDSVLPGVYNKDLDDFKKKLRFYVIHLKDLQPVSVSRPPQPTSSSDNFRPMTVEC","proteinLength":"Full Length","predictedMolecularWeight":"16.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":166,"aminoAcidStart":27,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":null,"tags":[]}]
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Properties and storage information

Form
Lyophilized
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
True
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General info

Function

Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Also stimulates mature basophils, eosinophils, and monocytes to become functionally activated. In addition, plays an important role in neural cell proliferation and survival. Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation (PubMed : 34183475). Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB (By similarity). Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program. Alternatively, contributes to cell survival under oxidative stress in non-hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK (By similarity).

Sequence similarities

Belongs to the IL-3 family.

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Product protocols

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Target data

Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Also stimulates mature basophils, eosinophils, and monocytes to become functionally activated. In addition, plays an important role in neural cell proliferation and survival. Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation (PubMed : 34183475). Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB (By similarity). Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program. Alternatively, contributes to cell survival under oxidative stress in non-hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK (By similarity).
See full target information Il3
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Product promise

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