Recombinant Rat IL-33 protein
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Recombinant Rat IL-33 protein is a Rat Fragment protein, in the 109 to 264 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
View Alternative Names
Interleukin-33, IL-33, Il33
- WB
Supplier Data
Western blot - Recombinant Rat IL-33 protein (AB207126)
All lanes:
Western blot - Anti-IL-33 antibody [EPR20417] (<a href='/en-us/products/primary-antibodies/il-33-antibody-epr20417-ab207737'>ab207737</a>) at 1/1000 dilution
All lanes:
Western blot - Recombinant Rat IL-33 protein (ab207126)
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 30 kDa
Observed band size: 20 kDa
false
Exposure time: 3min
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Rat IL-33 protein (AB207126)
15% SDS-PAGE analysis of ab207126 (3 μg)
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Specifications
Form
Liquid
Additional notes
ab207126 was purified using conventional chromatography.
General info
Function
Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury (By similarity). Induces rapid UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity).. In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or pro-inflammatory activation (By similarity).
Sequence similarities
Belongs to the IL-1 family. Highly divergent.
Post-translational modifications
The full-length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CELA1, CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full-length protein. May also be proteolytically processed by calpains. Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation. In vitro proteolytic cleavage by CASP1 was reported but could not be confirmed in vivo suggesting that IL33 is probably not a direct substrate for that caspase (By similarity).
Subcellular localisation
Nucleus
Target data
Complementary Products
Primary Antibodies
AB187060
Anti-IL-33 antibody [EPR17831]
BOND RX™ Validated|KO Validated|RabMAb|Recombinant|20ul selling size
![Immunocytochemistry/ Immunofluorescence - Anti-IL-33 antibody [EPR17831] (AB187060)](https://content.abcam.com/products/images/il-33-antibody-epr17831-ab187060--immunocytochemistry-immunofluorescence-img87072.jpg)
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Product promise
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