Recombinant Rat IL-33 protein is a Rat Fragment protein, in the 109 to 264 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
>90% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE, MS
No
M G S S H H H H H H S S G L V P R G S H M G S S I Q G T S L L T E S C A L S T Y N D Q S V S F V L E N G C Y V I N V E D C G K N Q E K D K V L L R Y Y E S S F P A Q S G D G V D G K K L M V N M S P I K D T D I W L N A N D K D Y S V E L Q K G D V S P P D Q A F F V L H K K S S D F V S F E C K N L P G T Y I G V K D N Q L A L V E E N D E S C N N I M F K L S K M
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury (By similarity). Induces rapid UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity).In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or pro-inflammatory activation (By similarity).
Interleukin-33, IL-33, Il33
Recombinant Rat IL-33 protein is a Rat Fragment protein, in the 109 to 264 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
>90% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE, MS
No
No
Rat
pH: 7.4
Constituents: 89% PBS, 10% Glycerol (glycerin, glycerine)
M G S S H H H H H H S S G L V P R G S H M G S S I Q G T S L L T E S C A L S T Y N D Q S V S F V L E N G C Y V I N V E D C G K N Q E K D K V L L R Y Y E S S F P A Q S G D G V D G K K L M V N M S P I K D T D I W L N A N D K D Y S V E L Q K G D V S P P D Q A F F V L H K K S S D F V S F E C K N L P G T Y I G V K D N Q L A L V E E N D E S C N N I M F K L S K M
Fragment
19.8 kDa
109 to 264
Recombinant
His tag N-Terminus
Liquid
ab207126 was purified using conventional chromatography.
Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury (By similarity). Induces rapid UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity).
Belongs to the IL-1 family. Highly divergent.
The full-length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CELA1, CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full-length protein. May also be proteolytically processed by calpains. Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation. In vitro proteolytic cleavage by CASP1 was reported but could not be confirmed in vivo suggesting that IL33 is probably not a direct substrate for that caspase (By similarity).
Nucleus
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
15% SDS-PAGE analysis of ab207126 (3 μg)
All lanes: Western blot - Anti-IL-33 antibody [EPR20417] (Anti-IL-33 antibody [EPR20417] ab207737) at 1/1000 dilution
All lanes: Western blot - Recombinant Rat IL-33 protein (ab207126)
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 30 kDa
Observed band size: 20 kDa
Exposure time: 3min
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com