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Recombinant Rat IL-33 protein is a Rat Fragment protein, in the 109 to 264 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.

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Images

SDS-PAGE - Recombinant Rat IL-33 protein (AB207126), expandable thumbnail
  • Western blot - Recombinant Rat IL-33 protein (AB207126), expandable thumbnail

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, MS

Biologically active

No

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M G S S I Q G T S L L T E S C A L S T Y N D Q S V S F V L E N G C Y V I N V E D C G K N Q E K D K V L L R Y Y E S S F P A Q S G D G V D G K K L M V N M S P I K D T D I W L N A N D K D Y S V E L Q K G D V S P P D Q A F F V L H K K S S D F V S F E C K N L P G T Y I G V K D N Q L A L V E E N D E S C N N I M F K L S K M

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

MS

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury (By similarity). Induces rapid UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity).In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or pro-inflammatory activation (By similarity).

Alternative names

Recommended products

Recombinant Rat IL-33 protein is a Rat Fragment protein, in the 109 to 264 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE, MS

Accession
Q66H70-1
Animal free

No

Species

Rat

Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 89% PBS, 10% Glycerol (glycerin, glycerine)

Sequence info

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M G S S I Q G T S L L T E S C A L S T Y N D Q S V S F V L E N G C Y V I N V E D C G K N Q E K D K V L L R Y Y E S S F P A Q S G D G V D G K K L M V N M S P I K D T D I W L N A N D K D Y S V E L Q K G D V S P P D Q A F F V L H K K S S D F V S F E C K N L P G T Y I G V K D N Q L A L V E E N D E S C N N I M F K L S K M

Accession

Q66H70

Protein length

Fragment

Predicted molecular weight

19.8 kDa

Amino acids

109 to 264

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Liquid

Additional notes

ab207126 was purified using conventional chromatography.

General info

Function

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury (By similarity). Induces rapid UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity).

Sequence similarities

Belongs to the IL-1 family. Highly divergent.

Post-translational modifications

The full-length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CELA1, CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full-length protein. May also be proteolytically processed by calpains. Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation. In vitro proteolytic cleavage by CASP1 was reported but could not be confirmed in vivo suggesting that IL33 is probably not a direct substrate for that caspase (By similarity).

Subcellular localisation

Nucleus

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Product promise

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2 product images

  • SDS-PAGE - Recombinant Rat IL-33 protein (ab207126), expandable thumbnail

    SDS-PAGE - Recombinant Rat IL-33 protein (ab207126)

    15% SDS-PAGE analysis of ab207126 (3 μg)

  • Western blot - Recombinant Rat IL-33 protein (ab207126), expandable thumbnail

    Western blot - Recombinant Rat IL-33 protein (ab207126)

    All lanes: Western blot - Anti-IL-33 antibody [EPR20417] (Anti-IL-33 antibody [EPR20417] ab207737) at 1/1000 dilution

    All lanes: Western blot - Recombinant Rat IL-33 protein (ab207126)

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 30 kDa

    Observed band size: 20 kDa

    Exposure time: 3min

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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