Recombinant rat IL-4 protein (Active) is a Rat Full Length protein, in the 25 to 147 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC.
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Key facts
- Purity
>=95% HPLC
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, MS, HPLC
- Biologically active
Yes
Amino acid sequence
C N D S P L R E I I N T L N Q V T E K G T P C T E M F V P D V L T A T R N T T E N E L I C R A S R V L R K F Y F P R D V P P C L K N K S G V L G E L R K L C R G V S G L N S L R S C T V N E S T L T T L K D F L E S L K S I L R G K Y L Q S C T S M S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
Alternative names
Interleukin-4, IL-4, B-cell IGG differentiation factor, B-cell growth factor 1, B-cell stimulatory factor 1, Lymphocyte stimulatory factor 1, BSF-1, Il-4, Il4
Recommended products
Recombinant rat IL-4 protein (Active) is a Rat Full Length protein, in the 25 to 147 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC.
Alternative names
Interleukin-4, IL-4, B-cell IGG differentiation factor, B-cell growth factor 1, B-cell stimulatory factor 1, Lymphocyte stimulatory factor 1, BSF-1, Il-4, Il4
Key facts
- Purity
>=95% HPLC
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, MS, HPLC
- Biologically active
Yes
- Biological activity
Fully biologically active determined by dose dependent increase in proliferation of rat splenocytes.
ED50 for this effect is ≤ 0.66 ng/ml corresponding to specific activity of 1.51 X 106 units/mg.
- Accession
- P20096-1
- Animal free
Yes
- Species
Rat
- Reconstitution
Store lyophilized form at room temperature. Reconstitute in PBS, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.428% Potassium phosphate monobasic
Sequence info
Amino acid sequence
C N D S P L R E I I N T L N Q V T E K G T P C T E M F V P D V L T A T R N T T E N E L I C R A S R V L R K F Y F P R D V P P C L K N K S G V L G E L R K L C R G V S G L N S L R S C T V N E S T L T T L K D F L E S L K S I L R G K Y L Q S C T S M S
- Accession
- Protein length
Full Length
- Predicted molecular weight
13.82 kDa
- Amino acids
25 to 147
- Nature
Recombinant
Specifications
- Form
Lyophilized
General info
- Function
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
- Sequence similarities
Belongs to the IL-4/IL-13 family.
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Functional Studies - Recombinant rat IL-4 protein (Active) (ab282395)
Functional analysis of ab282395.
Fully biologically active determined by dose dependent increase in proliferation of rat splenocytes.
ED50 for this effect is ≤ 0.66 ng/ml corresponding to specific activity of 1.51 X 106 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3393276-4
SDS-PAGE - Recombinant rat IL-4 protein (Active) (ab282395)
SDS-page analysis of ab282395.
Mass Spectrometry - Recombinant rat IL-4 protein (Active) (ab282395)
Mass determination by ESI-TOF.
Predicted MW is 13820.00 Da (+/- 10 Da by ESI-TOF). Observed MW is 13821.71
HPLC - Recombinant rat IL-4 protein (Active) (ab282395)
HPLC analysis of ab282395.
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