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AB282395

Recombinant rat IL-4 protein (Active)

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Recombinant rat IL-4 protein (Active) is a Rat Full Length protein, in the 25 to 147 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC.

View Alternative Names

Il-4, Il4, Interleukin-4, IL-4, B-cell IGG differentiation factor, B-cell growth factor 1, B-cell stimulatory factor 1, Lymphocyte stimulatory factor 1, BSF-1

4 Images
Functional Studies - Recombinant rat IL-4 protein (Active) (AB282395)
  • FuncS

Supplier Data

Functional Studies - Recombinant rat IL-4 protein (Active) (AB282395)

Functional analysis of ab282395.

Fully biologically active determined by dose dependent increase in proliferation of rat splenocytes.

ED50 for this effect is ≤ 0.66 ng/ml corresponding to specific activity of 1.51 X 106 units/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot : GR3393276-4

Mass Spectrometry - Recombinant rat IL-4 protein (Active) (AB282395)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant rat IL-4 protein (Active) (AB282395)

Mass determination by ESI-TOF.

Predicted MW is 13820.00 Da (+/- 10 Da by ESI-TOF). Observed MW is 13821.71

SDS-PAGE - Recombinant rat IL-4 protein (Active) (AB282395)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant rat IL-4 protein (Active) (AB282395)

SDS-page analysis of ab282395.

HPLC - Recombinant rat IL-4 protein (Active) (AB282395)
  • HPLC

Supplier Data

HPLC - Recombinant rat IL-4 protein (Active) (AB282395)

HPLC analysis of ab282395.

Key facts

Purity

>95% HPLC

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

Mass Spec, HPLC, SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by dose dependent increase in proliferation of rat splenocytes.

ED50 for this effect is ≤ 0.66 ng/ml corresponding to specific activity of 1.51 X 106 units/mg.

Accession

P20096

Animal free

Yes

Carrier free

No

Species

Rat

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.428% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"CNDSPLREIINTLNQVTEKGTPCTEMFVPDVLTATRNTTENELICRASRVLRKFYFPRDVPPCLKNKSGVLGELRKLCRGVSGLNSLRSCTVNESTLTTLKDFLESLKSILRGKYLQSCTSMS","proteinLength":"Full Length","predictedMolecularWeight":"13.82 kDa","actualMolecularWeight":null,"aminoAcidEnd":147,"aminoAcidStart":25,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P20096","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Specifications

Form

Lyophilized

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General info

Function

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.

Sequence similarities

Belongs to the IL-4/IL-13 family.

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Product protocols

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Target data

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
See full target information Il4
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Product promise

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