Recombinant Rat IL-7 Protein (Active) is a Rat Full Length protein, in the 26 to 154 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS, SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- HPLC, MS, SDS-PAGE, FuncS
- Biologically active
- No
Amino acid sequence
D C H I K D K D G K A F G S V L M I S I N Q L D K M T G T D S D C P N N E P N F F K K H L C D D T K E A A F L N R A A R K L R Q F L K M N I S E E F N D H L L R V S D G T Q T L V N C T S K E E K T I K E Q K K N D P C F L K R L L R E I K T C W N K I L K G S I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maintaining lymphoid homeostasis. Mechanistically, exerts its biological effects through a receptor composed of IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG. Binding to the receptor leads to activation of various kinases including JAK1 or JAK3 depending on the cell type and subsequently propagation of signals through activation of several downstream signaling pathways including the PI3K/Akt/mTOR or the JAK-STAT5.
Alternative names
Interleukin-7, IL-7, Il7
Recommended products
Recombinant Rat IL-7 Protein (Active) is a Rat Full Length protein, in the 26 to 154 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS, SDS-PAGE, FuncS.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- HPLC, MS, SDS-PAGE, FuncS
- Biologically active
- No
- Accession
- P56478-1
- Animal free
- Yes
- Species
- Rat
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- D C H I K D K D G K A F G S V L M I S I N Q L D K M T G T D S D C P N N E P N F F K K H L C D D T K E A A F L N R A A R K L R Q F L K M N I S E E F N D H L L R V S D G T Q T L V N C T S K E E K T I K E Q K K N D P C F L K R L L R E I K T C W N K I L K G S I
- Accession
- P56478
- Protein length
- Full Length
- Predicted molecular weight
- 14.94 kDa
- Actual molecular weight
- 14.94 kDa
- Amino acids
- 26 to 154
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
SDS-PAGE >=95%
General info
- Function
Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maintaining lymphoid homeostasis. Mechanistically, exerts its biological effects through a receptor composed of IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG. Binding to the receptor leads to activation of various kinases including JAK1 or JAK3 depending on the cell type and subsequently propagation of signals through activation of several downstream signaling pathways including the PI3K/Akt/mTOR or the JAK-STAT5.
- Sequence similarities
Belongs to the IL-7/IL-9 family.
- Post-translational modifications
Three disulfide bonds are present.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Interleukin-7 (IL-7) also known as IL-7 protein or IL-7 is a cytokine with a critical role in immune system development and function. IL-7 has an approximate molecular mass of 25 kDa and it exhibits expression in several tissues including stromal cells in the bone marrow and thymus. It interacts with its specific receptor IL-7R which consists of the IL-7Rα chain and the common gamma chain (γc) shared with other cytokine receptors. These interactions are important for signaling pathways that support lymphoid tissue homeostasis.
- Biological function summary
IL-7 significantly influences lymphocyte development and survival. It is essential for the proliferation and maintenance of T cells particularly thymocytes during T cell maturation and peripheral T cells after they exit the thymus. IL-7 does not form a complex itself but works as a critical single molecule in these processes. IL-7's role is especially noted in the maintenance of naïve and memory T cells important for adaptive immunity.
- Pathways
IL-7 activity impacts important immune signaling routes. The cytokine is an important player in the JAK/STAT signaling pathway which gets activated upon IL-7R engagement. This pathway is fundamental in controlling T cell development proliferation and survival. IL-7's interaction with other proteins such as the IL-7R complex and their downstream signaling partners integrates it into networks critical for strengthening the immune response.
- Associated diseases and disorders
IL-7 is significantly linked to immunodeficiencies and cancers affecting immune cells. For instance IL-7's altered signaling is associated with severe combined immunodeficiencies (SCID) where the lack of functional IL-7 or its receptor compromises T cell development. Additionally overexpression of IL-7 has connections to certain leukemias and lymphomas where it may contribute to inappropriate lymphocyte proliferation. In these contexts IL-7 often gets investigated alongside proteins such as the JAK and STAT family involved in tumorigenesis and immune dysregulation.
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4 product images
Mass Spectrometry - Recombinant Rat IL-7 Protein (Active) (ab290092)
Mass determination by ESI-TOF. Predicted MW is 14936.21 Da (+/-10 Da by ESI-TOF). Observed MW is 14938.09 Da.
Functional Studies - Recombinant Rat IL-7 Protein (Active) (ab290092)
Fully biologically active determined by the dose dependent proliferation of 2E8 cells.
ED50 is ≤ 16.3 ng/ml, corresponding to a specific activity of 6.1 x 10⁴ units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot. Lot GR3453026-1.SDS-PAGE - Recombinant Rat IL-7 Protein (Active) (ab290092)
SDS-PAGE analysis of ab290092
HPLC - Recombinant Rat IL-7 Protein (Active) (ab290092)
HPLC analysis of ab290092
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