Recombinant Rat IL-7 Protein (Active)
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Recombinant Rat IL-7 Protein (Active) is a Rat Full Length protein, in the 26 to 154 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for HPLC, Mass Spec, SDS-PAGE, FuncS.
View Alternative Names
Interleukin-7, IL-7, Il7
- FuncS
Supplier Data
Functional Studies - Recombinant Rat IL-7 Protein (Active) (AB290092)
Fully biologically active determined by the dose dependent proliferation of 2E8 cells. ED50 is ≤ 16.3 ng/ml, corresponding to a specific activity of 6.1 x 10⁴ units/mg. Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot. Lot GR3453026-1.
- Mass Spec
Supplier Data
Mass Spectrometry - Recombinant Rat IL-7 Protein (Active) (AB290092)
Mass determination by ESI-TOF. Predicted MW is 14936.21 Da (+/-10 Da by ESI-TOF). Observed MW is 14938.09 Da.
- HPLC
Supplier Data
HPLC - Recombinant Rat IL-7 Protein (Active) (AB290092)
HPLC analysis of ab290092
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Rat IL-7 Protein (Active) (AB290092)
SDS-PAGE analysis of ab290092
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
IL-7 significantly influences lymphocyte development and survival. It is essential for the proliferation and maintenance of T cells particularly thymocytes during T cell maturation and peripheral T cells after they exit the thymus. IL-7 does not form a complex itself but works as a critical single molecule in these processes. IL-7's role is especially noted in the maintenance of naïve and memory T cells important for adaptive immunity.
Pathways
IL-7 activity impacts important immune signaling routes. The cytokine is an important player in the JAK/STAT signaling pathway which gets activated upon IL-7R engagement. This pathway is fundamental in controlling T cell development proliferation and survival. IL-7's interaction with other proteins such as the IL-7R complex and their downstream signaling partners integrates it into networks critical for strengthening the immune response.
Specifications
Form
Lyophilized
Additional notes
SDS-PAGE >=95%
General info
Function
Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maintaining lymphoid homeostasis. Mechanistically, exerts its biological effects through a receptor composed of IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG. Binding to the receptor leads to activation of various kinases including JAK1 or JAK3 depending on the cell type and subsequently propagation of signals through activation of several downstream signaling pathways including the PI3K/Akt/mTOR or the JAK-STAT5.
Sequence similarities
Belongs to the IL-7/IL-9 family.
Post-translational modifications
Three disulfide bonds are present.
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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