Recombinant Rat IL3 Protein (Active) is a Rat Full Length protein, in the 27 to 166 aa range, expressed in HEK 293, with >=95% purity and <= 0.005 EU/µg endotoxin level.
Images
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Biologically active
- Yes
Amino acid sequence
I S D R G S D A H H L L R T L D C R T I A L E I L V K L P V S G L N N S D D K A N L R N S T L R R V N L D E F L K S Q E E F D S Q D T T D I K S K L Q K L K C C I P A A A S D S V L P G V Y N K D L D D F K K K L R F Y V I H L K D L Q P V S V S R P P Q P T S S S D N F R P M T V E C
Target data
Function
Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Stimulates also mature basophils, eosinophils, and monocytes to become functionally activated. In addition, plays an important role in neural cell proliferation and survival. Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation (PubMed:34183475). Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB (By similarity). Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program. Alternatively, contributes to cell survival under oxidative stress in non-hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK (By similarity).
Alternative names
Il-3, Il3, Interleukin-3, IL-3, Hematopoietic growth factor, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor, MCGF
Recommended products
Recombinant Rat IL3 Protein (Active) is a Rat Full Length protein, in the 27 to 166 aa range, expressed in HEK 293, with >=95% purity and <= 0.005 EU/µg endotoxin level.
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by dose dependent proliferation of NFS-60 cells.
ED50 is ≤ 8.3 ng/ml, corresponding to a specific activity of 1.2 x 105 units/mg.
- Accession
- P04823-1
- Animal free
- No
- Species
- Rat
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- I S D R G S D A H H L L R T L D C R T I A L E I L V K L P V S G L N N S D D K A N L R N S T L R R V N L D E F L K S Q E E F D S Q D T T D I K S K L Q K L K C C I P A A A S D S V L P G V Y N K D L D D F K K K L R F Y V I H L K D L Q P V S V S R P P Q P T S S S D N F R P M T V E C
- Accession
- P04823
- Protein length
- Full Length
- Amino acids
- 27 to 166
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Stimulates also mature basophils, eosinophils, and monocytes to become functionally activated. In addition, plays an important role in neural cell proliferation and survival. Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation (PubMed:34183475). Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB (By similarity). Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program. Alternatively, contributes to cell survival under oxidative stress in non-hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK (By similarity).
- Sequence similarities
Belongs to the IL-3 family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Rat IL3 Protein (Active) (ab290083)
SDS-PAGE analysis of ab290083
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Product protocols
- Visit the general protocols
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