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Recombinant rat IP10 protein is a Rat Full Length protein, in the 22 to 98 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB, FuncS.

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Images

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, WB, FuncS

Biologically active

Yes

Amino acid sequence

I P L A R T V R C T C I D F H E Q P L R P R A I G K L E I I P A S L S C P H V E I I A T M K K N N E K R C L N P E S E A I K S L L K A V S Q R R S K R A P

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Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

Ab75477 can be used as a WB positive control in conjunction with ab9807.

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects (By similarity). Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites (By similarity). Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization. In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation (By similarity). Activation of the CXCL10/CXCR3 axis plays also an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization (By similarity) (PubMed:30257241, PubMed:30448292).

Alternative names

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Recombinant rat IP10 protein is a Rat Full Length protein, in the 22 to 98 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB, FuncS.

Alternative names

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE, WB, FuncS

Biological activity

Active

Accession
P48973-1
Animal free

No

Species

Rat

Reconstitution

Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Concentration
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Sequence info

Amino acid sequence

I P L A R T V R C T C I D F H E Q P L R P R A I G K L E I I P A S L S C P H V E I I A T M K K N N E K R C L N P E S E A I K S L L K A V S Q R R S K R A P

Accession

P48973

Protein length

Full Length

Amino acids

22 to 98

Nature

Recombinant

Specifications

Form

Lyophilized

Additional notes

Purity : Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per µg (1EU/µg).

General info

Function

Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects (By similarity). Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites (By similarity). Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization. In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation (By similarity). Activation of the CXCL10/CXCR3 axis plays also an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization (By similarity) (PubMed:30257241, PubMed:30448292).

Sequence similarities

Belongs to the intercrine alpha (chemokine CxC) family.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Supplementary info

Biological function summary

IP10 plays a role in modulating the activities of immune cells. It attracts T cells eosinophils monocytes and natural killer (NK) cells by binding to the CXCR3 receptor. IP10 is not part of a larger complex but interacts with other cytokines to influence cell migration and the immune response. High levels of IP10 can reflect strong immune activation which is why it is often measured in inflammatory conditions using standard assays like the IP-10 ELISA kits.

Activity summary

IP10 also known as CXCL10 is a small cytokine belonging to the CXC chemokine family. It has a molecular mass of approximately 8.7 kDa. IP10 is secreted by several cell types such as monocytes endothelial cells and fibroblasts in response to interferon-gamma (IFN-γ). This protein is involved in immune responses and exhibits various roles especially in chemoattracting cells. Researchers often measure IP10 concentrations using ELISA kits such as the IP-10 ELISA to study its expression levels in different biological contexts.

Pathways

The role of IP10 lies within the Th1-type immune response pathway. In this pathway IP10 works alongside other chemokines to recruit and activate immune cells to sites of inflammation or infection. It synergizes with IFN-γ to propagate immune signals. IP10 is also linked with the CXCR3 receptor which plays a critical role in these pathways providing a connection to other proteins such as CXCL9 and CXCL11 which have similar functions in cell-mediated immunity.

Associated diseases and disorders

IP10 is associated with conditions like multiple sclerosis and rheumatoid arthritis. Elevated IP10 levels often correlate with disease activity in these disorders making it a potential biomarker for disease progression. The protein interacts with other inflammatory mediators such as TNF-α in regulating immune activity within these disease contexts. IP10's involvement in recruiting immune cells contributes to the pathogenic inflammation observed in these conditions.

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1 product image

  • Western blot - Recombinant rat IP10 protein (ab75477), expandable thumbnail

    Western blot - Recombinant rat IP10 protein (ab75477)

    All lanes: Western blot - Anti-IP10 antibody (AB9807) at 1 µg/mL

    All lanes: Western blot - Recombinant rat IP10 protein (AB75477) at 0.1 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 10 kDa

    Exposure time: 30s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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