Recombinant Rat M-CSF Protein (Active) is a Rat Fragment protein, in the 33 to 190 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC.
Images
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, MS, HPLC
- Biologically active
- Yes
Amino acid sequence
E V S E H C S H M I G N G H L Q I L Q Q L I D S Q M E T A C L I E Y K F V D Q E Q L D D P V C Y L K K A F V L V Q V I I E E T M R F K D N T P N A N A T E R L Q E L S M K L N S C F I K D Y K E Q N E A C V Q T Y K E S P L R L L E K I K N F F N E T K N F L E K D W N I F S K N C N D S F A K C S S R D V V T K P D C N C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance (By similarity).
Alternative names
Csfm, Csf1, Macrophage colony-stimulating factor 1, CSF-1, MCSF, Proteoglycan macrophage colony-stimulating factor, PG-M-CSF
Recommended products
Recombinant Rat M-CSF Protein (Active) is a Rat Fragment protein, in the 33 to 190 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, MS, HPLC
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by the dose dependent proliferation of M-NFS-60 cells. ED50 is ≤ 1.8 ng/ml, corresponding to a specific activity of 5.5 x 105 units/mg.
- Accession
- Q8JZQ0-1
- Animal free
- Yes
- Species
- Rat
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- E V S E H C S H M I G N G H L Q I L Q Q L I D S Q M E T A C L I E Y K F V D Q E Q L D D P V C Y L K K A F V L V Q V I I E E T M R F K D N T P N A N A T E R L Q E L S M K L N S C F I K D Y K E Q N E A C V Q T Y K E S P L R L L E K I K N F F N E T K N F L E K D W N I F S K N C N D S F A K C S S R D V V T K P D C N C
- Accession
- Q8JZQ0
- Protein length
- Fragment
- Predicted molecular weight
- 18.5 kDa
- Actual molecular weight
- 18.5 kDa
- Amino acids
- 33 to 190
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
SDS-PAGE >= 95%
General info
- Function
Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance (By similarity).
- Post-translational modifications
N-glycosylated.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
M-CSF or macrophage colony-stimulating factor is a cytokine involved in the regulation of macrophage production. Alternative names for M-CSF include CSF-1 and colony-stimulating factor 1. The M-CSF protein has a molecular mass of approximately 18 to 22 kDa. M-CSF is mainly expressed in various tissues including the placenta kidney and bone marrow. It functions as a homodimer important for the survival proliferation and differentiation of mononuclear phagocyte lineage cells.
- Biological function summary
M-CSF influences the production differentiation and function of macrophages and osteoclasts. It is not part of a large complex but interacts with its receptor the CSF1R (colony-stimulating factor 1 receptor) on the surface of target cells. This interaction promotes the survival and proliferation of the target cells playing significant roles in immune response and bone homeostasis by regulating osteoclast development.
- Pathways
The interaction of M-CSF with its receptor is central to several biological pathways notably the immune system pathway and bone resorption pathway. Within these pathways the binding of M-CSF to CSF1R activates downstream signaling cascades such as the PI3K/AKT and MAPK pathways importantly affecting cell survival and differentiation. The M-CSF pathway intersects with other cytokines and factors like GM-CSF (granulocyte-macrophage colony-stimulating factor) which also regulate immune cell dynamics.
- Associated diseases and disorders
Dysregulation of M-CSF levels is implicated in conditions such as osteoporosis and certain cancers. In osteoporosis M-CSF’s role in osteoclast development links to increased bone resorption leading to bone loss. In cancers M-CSF overexpression may facilitate tumor-associated macrophage infiltration therefore supporting tumor progression. The interaction with CSF1R is significant as it serves as a potential target for therapeutic strategies aimed at modulating macrophage activity in these diseases.
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4 product images
SDS-PAGE - Recombinant Rat M-CSF Protein (Active) (ab307410)
SDS-PAGE analysis of ab307410
Biological Activity - Recombinant Rat M-CSF Protein (Active) (ab307410)
Fully biologically active determined by the dose dependent proliferation of M-NFS-60 cells. ED50 is ≤ 1.8 ng/ml, corresponding to a specific activity of 5.5 x 10^5 units/mg.
HPLC - Recombinant Rat M-CSF Protein (Active) (ab307410)
HPLC analysis of ab307410
Mass Spectrometry - Recombinant Rat M-CSF Protein (Active) (ab307410)
Mass determination by ESI-TOF. Predicted MW is 18505.07 Da (+/-10 Da by ESI-TOF). Observed MW is 18506.60 Da.
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