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Recombinant Rat M-CSF Protein (Active) is a Rat Fragment protein, in the 33 to 190 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC.
Alternative names=Macrophage colony-stimulating factor 1, CSF-1, MCSF, Csfm, Csf1
- Purity
>=95% HPLC
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, MS, HPLC
- Biologically active
Yes
- Actual molecular weight
18.5 kDa
- Sequence
E V S E H C S H M I G N G H L Q I L Q Q L I D S Q M E T A C L I E Y K F V D Q E Q L D D P V C Y L K K A F V L V Q V I I E E T M R F K D N T P N A N A T E R L Q E L S M K L N S C F I K D Y K E Q N E A C V Q T Y K E S P L R L L E K I K N F F N E T K N F L E K D W N I F S K N C N D S F A K C S S R D V V T K P D C N C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant Rat M-CSF Protein (Active) is a Rat Fragment protein, in the 33 to 190 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC.
Alternative names=Macrophage colony-stimulating factor 1, CSF-1, MCSF, Csfm, Csf1
Key facts
- Purity
>=95% HPLC
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, MS, HPLC
- Biologically active
Yes
- Biological activity
Fully biologically active determined by the dose dependent proliferation of M-NFS-60 cells. ED50 is ≤ 1.8 ng/ml, corresponding to a specific activity of 5.5 x 105 units/mg.
- Predicted molecular weight
18.5 kDa
- Actual molecular weight
18.5 kDa
- Amino acids
33 to 190
- Sequence
E V S E H C S H M I G N G H L Q I L Q Q L I D S Q M E T A C L I E Y K F V D Q E Q L D D P V C Y L K K A F V L V Q V I I E E T M R F K D N T P N A N A T E R L Q E L S M K L N S C F I K D Y K E Q N E A C V Q T Y K E S P L R L L E K I K N F F N E T K N F L E K D W N I F S K N C N D S F A K C S S R D V V T K P D C N C
- Accession
- Q8JZQ0-1
- Protein length
Fragment
- Animal free
Yes
- Nature
Recombinant
- Species
Rat
- Reconstitution
Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Specifications
- Form
Lyophilized
- Additional notes
SDS-PAGE >= 95%
General info
- Function
Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance (By similarity).
- Post-translational modifications
N-glycosylated. The predominant soluble form is a chondroitin sulfate-containing proteoglycan (By similarity).
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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