Recombinant rat M-CSF protein (Animal Free) is a Rat Fragment protein, in the 33 to 186 aa range, expressed in Escherichia coli, with =95% purity, < 0.05 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
=95% SDS-PAGE
- Endotoxin level
< 0.05 EU/µg
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
Amino acid sequence
M E V S E H C S H M I G N G H L Q I L Q Q L I D S Q M E T A C L I E Y K F V D Q E Q L D D P V C Y L K K A F V L V Q V I I E E T M R F K D N T P N A N A T E R L Q E L S M K L N S C F I K D Y K E Q N E A C V Q T Y K E S P L R L L E K I K N F F N E T K N F L E K D W N I F S K N C N D S L A K C S S R D V V T K P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance (By similarity).
Alternative names
Macrophage colony-stimulating factor 1, CSF-1, MCSF, Csfm, Csf1
Recommended products
Recombinant rat M-CSF protein (Animal Free) is a Rat Fragment protein, in the 33 to 186 aa range, expressed in Escherichia coli, with =95% purity, < 0.05 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Alternative names
Macrophage colony-stimulating factor 1, CSF-1, MCSF, Csfm, Csf1
Key facts
- Purity
=95% SDS-PAGE
- Endotoxin level
< 0.05 EU/µg
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
NFS-60 cell proliferation: ED50 ≤10 ng/ml; ≥ 1.0 x 105 units/mg.
- Accession
- Q8JZQ0-1
- Animal free
Yes
- Carrier free
Yes
- Species
Rat
- Reconstitution
Reconstitute with sterile water to 0.1 mg/mL. Centrifuge vial before opening. Suspend the product by gently pipetting down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. Dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
- Concentration
- Storage buffer
Constituents: 0.16% Sodium phosphate
Sequence info
Amino acid sequence
M E V S E H C S H M I G N G H L Q I L Q Q L I D S Q M E T A C L I E Y K F V D Q E Q L D D P V C Y L K K A F V L V Q V I I E E T M R F K D N T P N A N A T E R L Q E L S M K L N S C F I K D Y K E Q N E A C V Q T Y K E S P L R L L E K I K N F F N E T K N F L E K D W N I F S K N C N D S L A K C S S R D V V T K P
- Accession
- Protein length
Fragment
- Predicted molecular weight
36.2 kDa
- Amino acids
33 to 186
- Nature
Recombinant
Specifications
- Form
Lyophilized
- Additional notes
Purity is typically 95% as determined by reducing and non-reducing SDS-PAGE.
General info
- Function
Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance (By similarity).
- Post-translational modifications
N-glycosylated. The predominant soluble form is a chondroitin sulfate-containing proteoglycan (By similarity).
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
M-CSF or macrophage colony-stimulating factor is a cytokine involved in the regulation of macrophage production. Alternative names for M-CSF include CSF-1 and colony-stimulating factor 1. The M-CSF protein has a molecular mass of approximately 18 to 22 kDa. M-CSF is mainly expressed in various tissues including the placenta kidney and bone marrow. It functions as a homodimer important for the survival proliferation and differentiation of mononuclear phagocyte lineage cells.
- Biological function summary
M-CSF influences the production differentiation and function of macrophages and osteoclasts. It is not part of a large complex but interacts with its receptor the CSF1R (colony-stimulating factor 1 receptor) on the surface of target cells. This interaction promotes the survival and proliferation of the target cells playing significant roles in immune response and bone homeostasis by regulating osteoclast development.
- Pathways
The interaction of M-CSF with its receptor is central to several biological pathways notably the immune system pathway and bone resorption pathway. Within these pathways the binding of M-CSF to CSF1R activates downstream signaling cascades such as the PI3K/AKT and MAPK pathways importantly affecting cell survival and differentiation. The M-CSF pathway intersects with other cytokines and factors like GM-CSF (granulocyte-macrophage colony-stimulating factor) which also regulate immune cell dynamics.
- Associated diseases and disorders
Dysregulation of M-CSF levels is implicated in conditions such as osteoporosis and certain cancers. In osteoporosis M-CSF’s role in osteoclast development links to increased bone resorption leading to bone loss. In cancers M-CSF overexpression may facilitate tumor-associated macrophage infiltration therefore supporting tumor progression. The interaction with CSF1R is significant as it serves as a potential target for therapeutic strategies aimed at modulating macrophage activity in these diseases.
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Cellular Activity - Recombinant rat M-CSF protein (Animal Free) (ab207964)
Dose-dependent induction of M-NFS-60 cell proliferation. Cells were incubated with ab207964, followed by measurement at 490 nm. The assay was carried out in duplicate.
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