Recombinant rat MCP1 protein (Active) is a Rat Full Length protein, in the 24 to 148 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
Q P D A V N A P L T C C Y S F T G K M I P M S R L E N Y K R I T S S R C P K E A V V F V T K L K R E I C A D P N K E W V Q K Y I R K L D Q N Q V R S E T T V F Y K I A S T L R T S A P L N V N L T H K S E A N A S T L F S T T T S S T S V E V T S M T E N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Acts as a ligand for C-C chemokine receptor CCR2 (By similarity). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (By similarity). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (By similarity). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (By similarity).
Alternative names
Je, Mcp1, Scya2, Ccl2, C-C motif chemokine 2, Immediate-early serum-responsive protein JE, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Small-inducible cytokine A2, MCP-1
Recommended products
Recombinant rat MCP1 protein (Active) is a Rat Full Length protein, in the 24 to 148 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- THP-1 chemotaxis (first detectable at 10 ng/mL).
- Accession
- P14844-1
- Animal free
- No
- Species
- Rat
- Reconstitution
- reconstitute with water at 0.1mg/mL
- Concentration
- Storage buffer
Constituents: 0.1% Trifluoroacetic acid
Sequence info
Amino acid sequence
- Q P D A V N A P L T C C Y S F T G K M I P M S R L E N Y K R I T S S R C P K E A V V F V T K L K R E I C A D P N K E W V Q K Y I R K L D Q N Q V R S E T T V F Y K I A S T L R T S A P L N V N L T H K S E A N A S T L F S T T T S S T S V E V T S M T E N
- Accession
- P14844
- Protein length
- Full Length
- Predicted molecular weight
- 11 kDa
- Amino acids
- 24 to 148
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
Acts as a ligand for C-C chemokine receptor CCR2 (By similarity). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (By similarity). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (By similarity). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (By similarity).
- Sequence similarities
Belongs to the intercrine beta (chemokine CC) family.
- Post-translational modifications
Processing at the N-terminus can regulate receptor and target cell selectivity (By similarity). Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant (By similarity).
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.
- Biological function summary
The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.
- Pathways
MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.
- Associated diseases and disorders
MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.
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1 product image
SDS-PAGE - Recombinant rat MCP1 protein (Active) (ab256242)
SDS-PAGE analysis of ab256242 (1μg) under non-reducing (Lane 1) and reducing (Lane 2) conditions.
ab256242 is predicted to have a MW of 14.1 kDa.
4-20% Tris-Glycine gel. Coomassie Blue staining.
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