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AB226888

Recombinant Rat Monoacylglycerol Lipase/MGL protein (His tag)

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Recombinant Rat Monoacylglycerol Lipase/MGL protein (His tag) is a Rat Full Length protein, in the 1 to 303 aa range, expressed in Yeast, with >90%, suitable for SDS-PAGE.

View Alternative Names

Mgl2, Mgll, Monoglyceride lipase, MGL, Monoacylglycerol lipase, MAGL

1 Images
SDS-PAGE - Recombinant Rat Monoacylglycerol Lipase/MGL protein (His tag) (AB226888)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Rat Monoacylglycerol Lipase/MGL protein (His tag) (AB226888)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab226888 with 5% enrichment gel and 15% separation gel.

Key facts

Purity

>90% SDS-PAGE

Expression system

Yeast

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q8R431

Animal free

No

Carrier free

No

Species

Rat

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Complementary Products

Primary Antibodies

AB24701

Anti-Monoacylglycerol Lipase/MGL antibody

Western blot - Anti-Monoacylglycerol Lipase/MGL antibody (AB24701)

  • 5
  • 12
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB273326

Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric)

Functional Studies - Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) (AB273326)

  • 0
  • 0
Primary Antibodies

AB300042

Anti-VISTA antibody [EPR25068-124]

BOND RX™ Validated|20ul selling size|KO Validated|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VISTA antibody [EPR25068-124] (AB300042)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as Monoacylglycerol Lipase
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Sequence info

[{"linker":null,"sequence":"MPEASSPRRTPQNVPYQDLPHLVNADGQYLFCRYWKPSGTPKALIFVSHGAGEHCGRYDELAQMLKRLDMLVFAHDHVGHGQSEGERMVVSDFQVFVRDLLQHVNTVQKDYPEVPVFLLGHSMGGAISILAAAERPTHFSGMILISPLILANPESASTLKVLAAKLLNFVLPNISLGRIDSSVLSRNKSEVDLYNSDPLICHAGVKVCFGIQLLNAVSRVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSPSQDKTLKMYEGAYHVLHKELPEVTNSVLHEINTWVSHRIAVAGARCLP","proteinLength":"Full Length","predictedMolecularWeight":"35.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":303,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Yeast","accessionNumber":"Q8R431","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Liquid
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Monoacylglycerol lipase (MGL) also known as MAGL or lipase 63 is an enzyme with a mass of approximately 33 kDa. MGL primarily functions in lipid metabolism by hydrolyzing 2-monoacylglycerol (2-MAG) into free fatty acids and glycerol. This enzyme is expressed in many tissues with high expression in the brain liver and adipose tissue. Its activity plays a significant role in the breakdown of monoacylglycerol an important intermediate in the endocannabinoid signaling system.
Biological function summary

MGL contributes to the regulation of energy homeostasis by controlling the levels of 2-monoacylglycerol. It catalyzes the final step in the degradation of the endocannabinoid 2-arachidonoylglycerol (2-AG) which is important for modulating inflammation pain and mood. MGL is not known to be part of a larger protein complex. In the brain its action helps terminate endocannabinoid signaling therefore influencing neurotransmission and synaptic plasticity.

Pathways

MGL plays a critical role in the endocannabinoid and glycerolipid metabolism pathways. In the endocannabinoid system it interacts with the cannabinoid receptors CB1 and CB2 by regulating 2-AG levels. The protein's role in glycerolipid metabolism involves its interaction with long-chain acyl-CoA synthetase and fatty acid oxidation pathways which are essential for maintaining lipid balance and cellular energy.

MGL has been linked to neurodegenerative diseases and cancer. In neurodegenerative diseases like Alzheimer’s dysregulation of MGL activity may lead to altered endocannabinoid signaling impacting neuronal health. In cancer increased MGL activity can promote tumor progression by providing cancer cells with fatty acids for growth and survival. MGL's interaction with proteins such as fatty acid synthase highlights its importance in cancer metabolism.
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General info

Function

Converts monoacylglycerides to free fatty acids and glycerol (PubMed : 12136125). Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (PubMed : 12136125, PubMed : 17649977). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth (By similarity).

Sequence similarities

Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family.

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Product protocols

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Target data

Converts monoacylglycerides to free fatty acids and glycerol (PubMed : 12136125). Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (PubMed : 12136125, PubMed : 17649977). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth (By similarity).
See full target information Mgll
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Product promise

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