Recombinant Rat p53 protein (His tag) is a Rat Full Length protein, in the 1 to 391 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M E D S Q S D M S I E L P L S Q E T F S C L W K L L P P D D I L P T T A T G S P N S M E D L F L P Q D V A E L L E G P E E A L Q V S A P A A Q E P G T E A P A P V A P A S A T P W P L S S S V P S Q K T Y Q G N Y G F H L G F L Q S G T A K S V M C T Y S I S L N K L F C Q L A K T C P V Q L W V T S T P P P G T R V R A M A I Y K K S Q H M T E V V R R C P H H E R C S D G D G L A P P Q H L I R V E G N P Y A E Y L D D R Q T F R H S V V V P Y E P P E V G S D Y T T I H Y K Y M C N S S C M G G M N R R P I L T I I T L E D S S G N L L G R D S F E V R V C A C P G R D R R T E E E N F R K K E E H C P E L P P G S A K R A L P T S T S S S P Q Q K K K P L D G E Y F T L K I R G R E R F E M F R E L N E A L E L K D A R A A E E S G D S R A H S S Y P K T K K G Q S T S R H K K P M I K K V G P D S D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence. Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Negatively regulates cell division by controlling expression of a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (By similarity). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (By similarity). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2.
Alternative names
P53, Cellular tumor antigen p53, Tumor suppressor p53
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Recombinant Rat p53 protein (His tag) is a Rat Full Length protein, in the 1 to 391 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P10361
- Animal free
- No
- Species
- Rat
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- M E D S Q S D M S I E L P L S Q E T F S C L W K L L P P D D I L P T T A T G S P N S M E D L F L P Q D V A E L L E G P E E A L Q V S A P A A Q E P G T E A P A P V A P A S A T P W P L S S S V P S Q K T Y Q G N Y G F H L G F L Q S G T A K S V M C T Y S I S L N K L F C Q L A K T C P V Q L W V T S T P P P G T R V R A M A I Y K K S Q H M T E V V R R C P H H E R C S D G D G L A P P Q H L I R V E G N P Y A E Y L D D R Q T F R H S V V V P Y E P P E V G S D Y T T I H Y K Y M C N S S C M G G M N R R P I L T I I T L E D S S G N L L G R D S F E V R V C A C P G R D R R T E E E N F R K K E E H C P E L P P G S A K R A L P T S T S S S P Q Q K K K P L D G E Y F T L K I R G R E R F E M F R E L N E A L E L K D A R A A E E S G D S R A H S S Y P K T K K G Q S T S R H K K P M I K K V G P D S D
- Accession
- P10361
- Protein length
- Full Length
- Predicted molecular weight
- 47.5 kDa
- Amino acids
- 1 to 391
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence. Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Negatively regulates cell division by controlling expression of a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (By similarity). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (By similarity). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2.
- Sequence similarities
Belongs to the p53 family.
- Post-translational modifications
Phosphorylation on Ser residues mediates transcriptional activation. Phosphorylation at Ser-9 by HIPK4 increases repression activity on BIRC5 promoter (By similarity). Phosphorylated on Thr-18 by VRK1, which may prevent the interaction with MDM2. Phosphorylated on Ser-20 by CHEK2 in response to DNA damage, which prevents ubiquitination by MDM2. Phosphorylated on Ser-20 by PLK3 in response to reactive oxygen species (ROS), promoting p53/TP53-mediated apoptosis. Probably phosphorylated on by CDK7 in a CAK complex in response to DNA damage. Phosphorylated by HIPK1. Phosphorylated on Ser-390 following UV but not gamma irradiation. Stabilized by CDK5-mediated phosphorylation in response to genotoxic and oxidative stresses at Ser-15, leading to accumulation of p53/TP53, particularly in the nucleus, thus inducing the transactivation of p53/TP53 target genes. Phosphorylated at Ser-313 and Ser-390 by CDK2 in response to DNA-damage (By similarity). Phosphorylation at Ser-15 is required for interaction with DDX3X and gamma-tubulin (By similarity). Phosphorylation at Ser-390 regulates its ability to undergo liquid-liquid phase separation by increasing fluidity of TP53/p53 condensates (By similarity).
- Subcellular localisation
- Nucleus, PML body, Mitochondrion matrix, Cytoskeleton, Microtubule organizing center, Centrosome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein p53 also known as TP53 or tumor protein p53 has a molecular weight of approximately 53 kDa. It acts as a transcription factor and plays a major role in cell cycle regulation apoptosis and maintaining genomic stability. This protein mainly expresses in the nucleus of cells and acts as a critical regulator of cellular responses to stress signals including DNA damage. Scientists commonly use p53 antibodies in various assays like western blot and p53 immunofluorescence to detect and study its expression and functional status in cells.
- Biological function summary
P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.
- Pathways
P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.
- Associated diseases and disorders
P53 mutation or inactivation is often associated with the development of cancer given its role in controlling cell division and preventing tumor formation. Specifically its dysfunction has been linked to cancers such as breast cancer and lung cancer. Additionally p53 can interact with other mutant proteins like Ras compounding mutations that contribute to tumor progression and aggressive cancer phenotypes. Understanding these interactions and the status of p53 can be important in developing targeted cancer therapies.
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1 product image
SDS-PAGE - Recombinant Rat p53 protein (His tag) (ab226419)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab226419 with 5% enrichment gel and 15% separation gel.
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