Recombinant rat PDE4D protein is a Rat Full Length protein, in the 2 to 803 aa range, expressed in Baculovirus infected Sf9, with >=80% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >=80% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M S P I L G Y W K I K G L V Q P T R L L L E Y L E E K Y E E H L Y E R D E G D K W R N K K F E L G L E F P N L P Y Y I D G D V K L T Q S M A I I R Y I A D K H N M L G G C P K E R A E I S M L E G A V L D I R Y G V S R I A Y S K D F E T L K V D F L S K L P E M L K M F E D R L C H K T Y L N G D H V T H P D F M L Y D A L D V V L Y M D P M C L D A F P K L V C F K K R I E A I P Q I D K Y L K S S K Y I A W P L Q G W Q A T F G G G D H P P K S D P L V P R G S P G E F E A E G S S V P A R A G S D E G S D S S G G A A L K A P K H L W R H E Q H H Q Y P L R Q P Q F R L L H P H H H L P P P P P P S P Q P Q L Q P P P P P P L P P P P P P P G A T R G R Y A S S G A S R V R H R G Y S D T E R Y L Y C R A M D R T S Y A V E T G H R P G L K K S R M S W P S S F Q G L R R F D V D N G T S A G R S P L D P M T S P G S G L I L Q A N F V H S Q R R E S F L Y R S D S D Y D L S P K S M S R N S S I A S D I H G D D L I V T P F A Q V L A S L R T V R N N F A A L T N L Q D R A P S K R S P M C N Q P S I N K A T I T E E A Y Q K L A S E T L E E L D W C L D Q L E T L Q T R H S V S E M A S N K F K R M L N R E L T H L S E M S R S G N Q V S E Y I S N T F L D K Q H E V E I P S P T Q K E K E K K K R P M S Q I S G V K K L M H S S S L T N S C I P R F G V K T E Q E D V L A K E L E D V N K W G L H V F R I A E L S G N R P L T V I M H T I F Q E R D L L K T F K I P V D T L I T Y L M T L E D H Y H A D V A Y H N N I H A A D V V Q S T H V L L S T P A L E A V F T D L E I L A A I F A S A I H D V D H P G V S N Q F L I N T N S E L A L M Y N D S S V L E N H H L A V G F K L L Q E E N C D I F Q N L T K K Q R Q S L R K M A I D I V L A T D M S K H M N L L A D L K T M V E T K K V T S S G V L L L D N Y S D R I Q V L Q N M V H C A D L S N P T K P L Q L Y R Q W T D R I M E E F F R Q G D R E R E R G M E I S P M C D K H N A S V E K S Q V G F I D Y I V H P L W E T W A D L V H P D A Q D I L D T L E D N R E W Y Q S T I P Q S P S P A P D D Q E D G R Q G Q T E K F Q F E L T L E E D G E S D T E K D S G S Q V E E D T S C S D S K T L C T Q D S E S T E I P L D E Q V E E E A V A E E E S Q P Q T G V A D D C C P D T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Alternative names
DPDE3, cAMP-specific phosphodiesterase 4D, Pde4d
Recommended products
Recombinant rat PDE4D protein is a Rat Full Length protein, in the 2 to 803 aa range, expressed in Baculovirus infected Sf9, with >=80% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=80% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific Activity: ≥ 22,535 pmole/min/μg.
Assay Description: One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C.
Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 μM cAMP, and serial dilutions of rPDE4D at 37°C for 20 min. Quantified by 5′ nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
- Accession
- P14270-5
- Animal free
- No
- Species
- Rat
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- M S P I L G Y W K I K G L V Q P T R L L L E Y L E E K Y E E H L Y E R D E G D K W R N K K F E L G L E F P N L P Y Y I D G D V K L T Q S M A I I R Y I A D K H N M L G G C P K E R A E I S M L E G A V L D I R Y G V S R I A Y S K D F E T L K V D F L S K L P E M L K M F E D R L C H K T Y L N G D H V T H P D F M L Y D A L D V V L Y M D P M C L D A F P K L V C F K K R I E A I P Q I D K Y L K S S K Y I A W P L Q G W Q A T F G G G D H P P K S D P L V P R G S P G E F E A E G S S V P A R A G S D E G S D S S G G A A L K A P K H L W R H E Q H H Q Y P L R Q P Q F R L L H P H H H L P P P P P P S P Q P Q L Q P P P P P P L P P P P P P P G A T R G R Y A S S G A S R V R H R G Y S D T E R Y L Y C R A M D R T S Y A V E T G H R P G L K K S R M S W P S S F Q G L R R F D V D N G T S A G R S P L D P M T S P G S G L I L Q A N F V H S Q R R E S F L Y R S D S D Y D L S P K S M S R N S S I A S D I H G D D L I V T P F A Q V L A S L R T V R N N F A A L T N L Q D R A P S K R S P M C N Q P S I N K A T I T E E A Y Q K L A S E T L E E L D W C L D Q L E T L Q T R H S V S E M A S N K F K R M L N R E L T H L S E M S R S G N Q V S E Y I S N T F L D K Q H E V E I P S P T Q K E K E K K K R P M S Q I S G V K K L M H S S S L T N S C I P R F G V K T E Q E D V L A K E L E D V N K W G L H V F R I A E L S G N R P L T V I M H T I F Q E R D L L K T F K I P V D T L I T Y L M T L E D H Y H A D V A Y H N N I H A A D V V Q S T H V L L S T P A L E A V F T D L E I L A A I F A S A I H D V D H P G V S N Q F L I N T N S E L A L M Y N D S S V L E N H H L A V G F K L L Q E E N C D I F Q N L T K K Q R Q S L R K M A I D I V L A T D M S K H M N L L A D L K T M V E T K K V T S S G V L L L D N Y S D R I Q V L Q N M V H C A D L S N P T K P L Q L Y R Q W T D R I M E E F F R Q G D R E R E R G M E I S P M C D K H N A S V E K S Q V G F I D Y I V H P L W E T W A D L V H P D A Q D I L D T L E D N R E W Y Q S T I P Q S P S P A P D D Q E D G R Q G Q T E K F Q F E L T L E E D G E S D T E K D S G S Q V E E D T S C S D S K T L C T Q D S E S T E I P L D E Q V E E E A V A E E E S Q P Q T G V A D D C C P D T
- Accession
- P14270
- Protein length
- Full Length
- Predicted molecular weight
- 117.2 kDa
- Amino acids
- 2 to 803
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
- Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
- Post-translational modifications
Isoform 1 and isoform 9 are rapidly activated by PKA through phosphorylation. Long isoforms that share a conserved PKA phosphorylation site in the N-terminus are also activated.
- Subcellular localisation
- Cytoskeleton, Microtubule organizing center, Centrosome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Phosphodiesterase 4D (PDE4D) is an enzyme that hydrolyzes cyclic AMP (cAMP) to its inactive form AMP controlling important cellular signaling pathways. It is part of the large PDE family and sometimes referred to as DPDE1. With a molecular weight of approximately 93 kDa PDE4D is widely expressed in the brain heart lungs and immune cells. Its expression in these tissues indicates an important regulatory role in both central and peripheral physiological processes.
- Biological function summary
PDE4D plays an important role in the modulation of intracellular cAMP levels impacting processes such as inflammation cognition and cardiac function. It does not operate as part of a larger complex but collaborates with other signaling molecules to influence cellular responses. PDE4D's regulation of cAMP levels directly affects protein kinase A (PKA) leading to alterations in gene expression and cell activity.
- Pathways
PDE4D integrates into the cAMP signaling and inflammatory pathways impacting various cellular responses. In the cAMP signaling pathway it partners with other PDEs and signaling proteins such as PKA which phosphorylates target proteins affecting cellular function. In inflammatory pathways PDE4D modulates immune cell activity by altering the cAMP-mediated inhibition of pro-inflammatory cytokine production linking it to inflammation-related responses.
- Associated diseases and disorders
PDE4D has associations with conditions such as asthma and ischemic stroke. In asthma PDE4D regulates cAMP levels in lung cells affecting bronchial constriction and inflammation. In ischemic stroke alterations in PDE4D activity influence vascular function by changing cAMP dynamics in vascular smooth muscle cells. Within these disorders PDE4D interacts with proteins like cytokines and COX-2 which also play parts in inflammatory pathways and vascular responses.
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2 product images
Functional Studies - Recombinant rat PDE4D protein (ab198442)
Activity assay of ab198442.
SDS-PAGE - Recombinant rat PDE4D protein (ab198442)
4-20% SDS-PAGE analysis of 2 μg ab198442 with Coomassie staining.
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