Recombinant Rat Plasma Kallikrein 1B protein (His tag) is a Rat Fragment protein, in the 391 to 638 aa range, expressed in Yeast, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Yeast
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
I V G G T N S S L G E W P W Q V S L Q V K L V S Q N H M C G G S I I G R Q W I L T A A H C F D G I P Y P D V W R I Y G G I L N L S E I T N K T P F S S I K E L I I H Q K Y K M S E G S Y D I A L I K L Q T P L N Y T E F Q K P I C L P S K A D T N T I Y T N C W V T G W G Y T K E R G E T Q N I L Q K A T I P L V P N E E C Q K K Y R D Y V I T K Q M I C A G Y K E G G I D A C K G D S G G P L V C K H S G R W Q L V G I T S W G E G C A R K E Q P G V Y T K V A E Y I D W I L E K I Q S S K E R A L E T S P A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
Alternative names
Klk3, Pk, Klkb1, Plasma kallikrein, Fletcher factor, Kininogenin, Plasma prekallikrein
Recommended products
Recombinant Rat Plasma Kallikrein 1B protein (His tag) is a Rat Fragment protein, in the 391 to 638 aa range, expressed in Yeast, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Yeast
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P14272-1
- Animal free
- No
- Species
- Rat
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- I V G G T N S S L G E W P W Q V S L Q V K L V S Q N H M C G G S I I G R Q W I L T A A H C F D G I P Y P D V W R I Y G G I L N L S E I T N K T P F S S I K E L I I H Q K Y K M S E G S Y D I A L I K L Q T P L N Y T E F Q K P I C L P S K A D T N T I Y T N C W V T G W G Y T K E R G E T Q N I L Q K A T I P L V P N E E C Q K K Y R D Y V I T K Q M I C A G Y K E G G I D A C K G D S G G P L V C K H S G R W Q L V G I T S W G E G C A R K E Q P G V Y T K V A E Y I D W I L E K I Q S S K E R A L E T S P A
- Accession
- P14272
- Protein length
- Fragment
- Predicted molecular weight
- 29.8 kDa
- Amino acids
- 391 to 638
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
- Sequence similarities
Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Plasma Kallikrein 1B also known as PKK or KLKB1 is a serine protease enzyme with an approximate mass of 88 kDa. It is important for the blood coagulation process. This protein is mainly expressed in the liver and circulates in the blood as the inactive precursor prekallikrein. Through cleavage prekallikrein converts into its active form plasma kallikrein. Plasma kallikrein exhibits various proteolytic activities influencing multiple substrates involved in hemostatic and inflammatory responses.
- Biological function summary
Plasma Kallikrein 1B plays significant roles in the kinin-kallikrein system. Within this system it cleaves high-molecular-weight kininogen to release bradykinin a potent vasodilator. This enzyme is not part of a larger protein complex but operates independently to modulate various physiological responses. Plasma kallikrein's activity influences processes like pain vasodilation and inflammation making it important in homeostasis and acute-phase responses.
- Pathways
Plasma Kallikrein 1B participates actively in the kallikrein-kinin and the contact activation pathways. In the kallikrein-kinin system it works alongside high-molecular-weight kininogen and factor XII which plays an important role in the intrinsic pathway of coagulation. Plasma kallikrein's enzymatic activities lead to the production of bradykinin and other peptides impacting blood pressure and inflammatory processes.
- Associated diseases and disorders
Plasma Kallikrein 1B associates strongly with hereditary angioedema and diabetic retinopathy. In hereditary angioedema its dysregulation leads to excessive bradykinin production causing episodes of swelling. In diabetic retinopathy elevated plasma kallikrein activity can exacerbate vascular complications. Plasma kallikrein's disorderly activities also connect it to other proteins like C1 inhibitor in these pathological conditions highlighting its importance in clinical studies.
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1 product image
SDS-PAGE - Recombinant Rat Plasma Kallikrein 1B protein (His tag) (ab226435)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab226435 with 5% enrichment gel and 15% separation gel.
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