Recombinant rat Prolactin/PRL protein (Active) is a Rat Full Length protein, in the 30 to 226 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M L P V C S G G D C Q T P L P E L F D R V V M L S H Y I H T L Y T D M F I E F D K Q Y V Q D R E F I A K A I N D C P T S S L A T P E D K E Q A Q K V P P E V L L N L I L S L V H S W N D P L F Q L I T G L G G I H E A P D A I I S R A K E I E E Q N K R L L E G I E K I I S Q A Y P E A K G N E I Y L V W S Q L P S L Q G V D E E S K D L A F Y N N I R C L R R D S H K V D N Y L K F L R C Q I V H K N N C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Prolactin acts primarily on the mammary gland by promoting lactation.
Additional Targets
Prolactin/PRL
Alternative names
Prolactin, PRL, Prl
Recommended products
Recombinant rat Prolactin/PRL protein (Active) is a Rat Full Length protein, in the 30 to 226 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- NB2-11 cell proliferation (typical ED50 is < 0.1 ng/mL).
- Accession
- P01237-1
- Animal free
- No
- Carrier free
- Yes
- Species
- Rat
- Reconstitution
- Reconstitute at 0.1 mg/mL in water
- Concentration
- Storage buffer
Constituents: 0.32% Sodium phosphate
Sequence info
Amino acid sequence
- M L P V C S G G D C Q T P L P E L F D R V V M L S H Y I H T L Y T D M F I E F D K Q Y V Q D R E F I A K A I N D C P T S S L A T P E D K E Q A Q K V P P E V L L N L I L S L V H S W N D P L F Q L I T G L G G I H E A P D A I I S R A K E I E E Q N K R L L E G I E K I I S Q A Y P E A K G N E I Y L V W S Q L P S L Q G V D E E S K D L A F Y N N I R C L R R D S H K V D N Y L K F L R C Q I V H K N N C
- Accession
- P01237
- Protein length
- Full Length
- Predicted molecular weight
- 26 kDa
- Amino acids
- 30 to 226
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
nan
General info
- Function
Prolactin acts primarily on the mammary gland by promoting lactation.
- Sequence similarities
Belongs to the somatotropin/prolactin family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Prolactin (PRL) also known as mammotropin is a peptide hormone with a molecular mass of approximately 23 kDa. PRL is primarily expressed in the anterior pituitary gland but can also be found in other tissues such as the mammary glands brain and immune system. PRL plays an important role in lactation stimulating milk production in mammals. The PRL protein functions by binding to the prolactin receptor a member of the cytokine receptor family initiating signal transduction pathways.
- Biological function summary
PRL influences a variety of physiological processes including reproduction metabolism and osmoregulation. It acts as a single entity rather than part of a larger protein complex affecting development and growth through its regulatory activities. In addition to lactation PRL also plays roles in the immune system cell proliferation and the production of surfactant in the lungs.
- Pathways
Prolactin is deeply involved in the JAK-STAT signaling pathway and the MAPK/ERK pathway which are pivotal for transmitting signals from the prolactin receptor to the nucleus. Through these pathways PRL interacts with proteins like Janus kinase (JAK) and Signal Transducer and Activator of Transcription (STAT) proteins facilitating its wide range of biological effects. This interplay helps regulate gene expression critical for various physiological conditions.
- Associated diseases and disorders
PRL is linked to conditions such as hyperprolactinemia and prolactinoma. Hyperprolactinemia characterized by elevated levels of prolactin in the blood can lead to symptoms such as galactorrhea and infertility. In the context of prolactinoma an adenoma of the pituitary gland PRL's overproduction is common. These disorders often relate to proteins like dopamine which typically acts to inhibit PRL secretion from the pituitary gland and any disruption in this regulation can result in the aforementioned conditions.
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1 product image
SDS-PAGE - Recombinant rat Prolactin/PRL protein (Active) (ab269197)
SDS-PAGE analysis of ab269197 at 1ug/lane under (-) non-reducing and (+) reducing conditions. 4-20% Tris glycine gel. Stained with coomassie blue.
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