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Recombinant Rat SCF Protein (Active) is a Rat Fragment protein, in the 26 to 189 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC.
Alternative names=Kit ligand, Hematopoietic growth factor KL, Mast cell growth factor, Stem cell factor, c-Kit ligand, MGF, SCF, Kitl, Mgf, Kitlg
- Purity
>=95% HPLC
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, MS, HPLC
- Biologically active
No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Images
Target data
Function
Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
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Recombinant Rat SCF Protein (Active) is a Rat Fragment protein, in the 26 to 189 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC.
Alternative names=Kit ligand, Hematopoietic growth factor KL, Mast cell growth factor, Stem cell factor, c-Kit ligand, MGF, SCF, Kitl, Mgf, Kitlg
Key facts
- Purity
>=95% HPLC
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, MS, HPLC
- Biologically active
No
- Amino acids
26 to 189
- Accession
- P21581-1
- Protein length
Fragment
- Animal free
No
- Nature
Recombinant
- Species
Rat
- Reconstitution
Reconstitute with phosphate buffered saline. Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1week. Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Specifications
- Form
Lyophilized
General info
- Function
Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
- Sequence similarities
Belongs to the SCF family.
- Post-translational modifications
A soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain.
- Subcellular localisation
Cytoskeleton
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
Ambient, -20°C
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