Recombinant rat SDF1 protein (Active) is a Full Length protein, in the 22 to 89 aa range, expressed in Escherichia coli, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, HPLC.
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- International journal of molecular sciences 21:2020IL-4 and SDF-1 Increase Adipose Tissue-Derived Stromal Cell Ability to Improve Rat Skeletal Muscle Regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMałgorzata Zimowska,Karolina Archacka,Edyta Brzoska,Joanna Bem,Areta M Czerwinska,Iwona Grabowska,Paulina Kasprzycka,Emilia Michalczewska,Igor Stepaniec,Marta Soszynska,Katarzyna Ilach,Wladyslawa Streminska,Maria A CiemerychPubMed 32392778
Key facts
- Purity
- >97% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, HPLC
- Biologically active
- Yes
Amino acid sequence
K P V S L S Y R C P C R F F E S H V A R A N V K H L K I L N T P N C A L Q I V A R L K S N N R Q V C I D P K L K W I Q E Y L D K A L N K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant rat SDF1 protein (Active) is a Full Length protein, in the 22 to 89 aa range, expressed in Escherichia coli, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, HPLC.
Key facts
- Purity
- >97% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, HPLC
- Biologically active
- Yes
- Biological activity
- Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 50-100 ng/ml.
- Animal free
- No
- Carrier free
- Yes
- Reconstitution
- Reconstitute in water or 0.1% BSA aqueous buffer
- Concentration
- Storage buffer
Constituents: PBS
Sequence info
Amino acid sequence
- K P V S L S Y R C P C R F F E S H V A R A N V K H L K I L N T P N C A L Q I V A R L K S N N R Q V C I D P K L K W I Q E Y L D K A L N K
- Protein length
- Full Length
- Predicted molecular weight
- 7.9 kDa
- Amino acids
- 22 to 89
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
>97% as determined by HPLC.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The stromal cell-derived factor 1 (SDF1) also known as C-X-C motif chemokine 12 (CXCL12) is a chemokine protein that is important in immunological responses and cellular signaling. This protein weighs approximately 8 kDa. SDF1 is largely expressed in bone marrow stroma liver and endothelium of various tissues positioning it as an integral player in cell migration and homing processes. The protein functions as a chemoattractant for lymphocytes promoting cellular trafficking and organ development.
- Biological function summary
SDF1 influences the migration and survival of hematopoietic progenitor cells. It plays a pivotal role in heart development angiogenesis and neuronal protein regulation. SDF1 binds with high affinity to its receptor CXCR4 forming a critical signal transduction complex that modulates cellular movement and growth responses. This interaction is important in the regulation of cell positioning and potential pathways of pathological changes.
- Pathways
SDF1 has an important role in the chemokine signaling pathway and is involved in the pathways controlling hematopoietic stem cell migration and homing. The interaction between SDF1 and CXCR4 triggers downstream signaling events engaging proteins like PI3K and MAPK which promote cell survival and proliferation. Furthermore the SDF1/CXCR4 axis is central to the vascular endothelial growth factor (VEGF) pathway facilitating angiogenesis and tissue repair mechanisms.
- Associated diseases and disorders
SDF1 relates closely to cancer metastasis and HIV infection. The SDF1/CXCR4 interaction acts as a co-receptor for HIV entry into host cells implicating it in viral pathogenesis. Overexpression of SDF1 and its binding partner CXCR4 contributes to tumor growth invasion and metastasis in various cancers by promoting angiogenesis and tumor cell migration. The targeting of the SDF1/CXCR4 axis holds therapeutic potential in cancer treatment and infectious disease management.
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SDS-PAGE - Recombinant rat SDF1 protein (Active) (ab243782)
SDS-PAGE analysis of ab243782
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