Recombinant Rat Sonic Hedgehog protein is a Rat Fragment protein, in the 26 to 198 aa range, expressed in Escherichia coli, with >95% purity, <= 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- <= 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M I I G P G R G F G K R Q H P K K L T P L A Y K Q F I P N V A E K T L G A S G R Y E G K I T R N S E R F K E L T P N Y N P D I I F K D E E N T G A D R L M T Q R C K D K L N A L A I S V M N Q W P G V K L R V T E G W D E D G H H S E E S L H Y E G R A V D I T T S D R D R S K Y G M L A R L A V E A G F D W V Y Y E S K A R I H C S V K A E N S V A A K S D G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Sonic hedgehog protein. The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity). Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN (By similarity). Both activities occur in the reticulum endoplasmic (By similarity). Once cleaved, ShhC is degraded in the endoplasmic reticulum (By similarity). Sonic hedgehog protein N-product. The dually lipidated sonic hedgehog protein N-product (ShhNp) is a morphogen which is essential for a variety of patterning events during development. Induces ventral cell fate in the neural tube and somites (By similarity). Involved in the patterning of the anterior-posterior axis of the developing limb bud (By similarity). Essential for axon guidance (By similarity). Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes (By similarity). In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO (By similarity).
Alternative names
Vhh-1, Shh, Sonic hedgehog protein, SHH, Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains, ShhNC
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Recombinant Rat Sonic Hedgehog protein is a Rat Fragment protein, in the 26 to 198 aa range, expressed in Escherichia coli, with >95% purity, <= 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- <= 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q63673-1
- Animal free
- No
- Species
- Rat
- Reconstitution
- Reconstitute at 0.1 mg/mL in water
- Concentration
- Storage buffer
Constituents: 0.16% Sodium phosphate
Sequence info
Amino acid sequence
- M I I G P G R G F G K R Q H P K K L T P L A Y K Q F I P N V A E K T L G A S G R Y E G K I T R N S E R F K E L T P N Y N P D I I F K D E E N T G A D R L M T Q R C K D K L N A L A I S V M N Q W P G V K L R V T E G W D E D G H H S E E S L H Y E G R A V D I T T S D R D R S K Y G M L A R L A V E A G F D W V Y Y E S K A R I H C S V K A E N S V A A K S D G
- Accession
- Q63673
- Protein length
- Fragment
- Predicted molecular weight
- 19.9 kDa
- Amino acids
- 26 to 198
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
Sonic hedgehog protein
- Sequence similarities
Belongs to the hedgehog family.
- Post-translational modifications
Sonic hedgehog protein
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Sonic Hedgehog also known as SHH is a protein that plays an important role in cell signaling during embryonic development. It has alternative names like 'HHG-1' and '5h4'. The Sonic Hedgehog protein is about 45 kDa in mass. Scientists find SHH expression in various tissues including the brain lungs and limb buds. Its activity plays a role in the organization of several tissue structures during development helping to regulate cellular growth and differentiation processes.
- Biological function summary
Sonic Hedgehog is critical for the vertebrate embryonic patterning and organogenesis. It functions as a signaling molecule and is part of the Hedgehog signaling complex. The protein acts by binding to the Patched receptor further influencing the Smoothened transducer in cellular signaling cascades. This signaling contributes to the proper formation of limbs central nervous system structures and other vital organs in early development stages.
- Pathways
Sonic Hedgehog participates in essential signaling pathways such as the Hedgehog pathway and the Wnt signaling pathway. It interacts with proteins including the Patched and Smoothened to facilitate downstream genetic transcription regulation. Through these pathways Sonic Hedgehog influences the transcription of specific genes that control cell growth. These interactions contribute to the regulation of different cell types and the organization of distinct tissue areas.
- Associated diseases and disorders
Sonic Hedgehog connects to issues like cancer and congenital malformations. Aberrations in its signaling can lead to conditions such as basal cell carcinoma and holoprosencephaly. Sonic Hedgehog through its influence on the Hedgehog signaling pathway implicates interactions with other proteins like GLI transcription factors and Patched. These interactions can dysregulate cellular processes and result in disease states when not functioning correctly.
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1 product image
SDS-PAGE - Recombinant Rat Sonic Hedgehog protein (ab233664)
1 μg ab233664 analyzed on a 4-20% Tris-Glycine gel, stained with Coomassie Blue.
Lane 1: Non-reducing conditions.
Lane 2: Reducing conditions.
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