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Recombinant rat sRANKL protein is a Rat Full Length protein, in the 145 to 318 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS.
Alternative names=Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF, OPGL, RANKL, TRANCE, Tnfsf11, Opgl, Rankl, Trance
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Sequence
P A M M E G S W L D V A R R G K P E A Q P F A H L T I N A A D I P S G S H K V S L S S W Y H D R G W A K I S N M T L S N G K L R V N Q D G F Y Y L Y A N I C F R H H E T S G S V P A D Y L Q L M V Y V V K T S I K I P S S H N L M K G G S T K N W S G N S E F H F Y S I N V G G F F K L R A G E E I S V Q V S N P S L L D P D Q D A T Y F G A F K V Q D I D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant rat sRANKL protein is a Rat Full Length protein, in the 145 to 318 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS.
Alternative names=Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF, OPGL, RANKL, TRANCE, Tnfsf11, Opgl, Rankl, Trance
Key facts
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
Determined by its ability to induce NF-κB in RAW264.7 cells in the absence of any cross-linking. The expected ED50 for this effect is 10.0-25.0 ng/ml.
- Amino acids
145 to 318
- Sequence
P A M M E G S W L D V A R R G K P E A Q P F A H L T I N A A D I P S G S H K V S L S S W Y H D R G W A K I S N M T L S N G K L R V N Q D G F Y Y L Y A N I C F R H H E T S G S V P A D Y L Q L M V Y V V K T S I K I P S S H N L M K G G S T K N W S G N S E F H F Y S I N V G G F F K L R A G E E I S V Q V S N P S L L D P D Q D A T Y F G A F K V Q D I D
- Accession
- Q9ESE2-1
- Protein length
Full Length
- Animal free
No
- Nature
Recombinant
- Species
Rat
- Concentration
Specifications
- Form
Lyophilized
- Additional notes
Purity : Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per µg (1EU/µg).
General info
- Function
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation.
- Sequence similarities
Belongs to the tumor necrosis factor family.
- Post-translational modifications
The soluble form derives from the membrane form by proteolytic processing.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
The biological activity of this product was determined by its ability to induce NFkappaB in RAW264.7 cells in the absence of any cross-linking. The expected ED50 for this effect is 10.0-25.0 ng/ml.
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