Recombinant rat TIGIT protein (Fc Chimera Active) is a Fragment protein, in the 17 to 138 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, ELISA.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, FuncS, ELISA
- Biologically active
- Yes
Amino acid sequence
A F L A A G A T A G T M E T K G N I S A E E G G S V V L Q C H F S S D T A E V T Q V N W E Q R D Q L L A V Y S V D L G W Y V P S V F S D R V V P G P S L G L T F Q S L T T N D T G E Y F C T Y H T Y P D G I Y K G R I F L K V Q E S S V A H F Q I A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant rat TIGIT protein (Fc Chimera Active) is a Fragment protein, in the 17 to 138 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, ELISA.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, FuncS, ELISA
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA. Immobilized human Nectin-2, His Tag at 5 μg/ml (100 μl/well) can bind ab224633 with a linear range of 0.039-5 μg/ml.
Measured by its binding ability in a BLI assay. Loaded ab224633 on Protein A Biosensor, can bind Human Nectin-2, His Tag with an affinity constant of 0.59 μM as determined.
- Animal free
- No
- Reconstitution
- Reconstitute at 400 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: 5% Trehalose, 0.87% Sodium chloride, 0.75% Glycine, 0.61% Tris, 0.44% L-Arginine
Sequence info
Amino acid sequence
- A F L A A G A T A G T M E T K G N I S A E E G G S V V L Q C H F S S D T A E V T Q V N W E Q R D Q L L A V Y S V D L G W Y V P S V F S D R V V P G P S L G L T F Q S L T T N D T G E Y F C T Y H T Y P D G I Y K G R I F L K V Q E S S V A H F Q I A
- Protein length
- Fragment
- Predicted molecular weight
- 39.9 kDa
- Amino acids
- 17 to 138
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
Specifications
- Form
- Lyophilized
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TIGIT also known as T cell immunoreceptor with Ig and ITIM domains is an immunoglobulin superfamily receptor. It weighs approximately 26 kDa. TIGIT commonly expresses on T cells especially on regulatory T cells (Tregs) and effector memory T cells. It also presents on natural killer (NK) cells. Among its alternate forms some notable ones also include the anti-TIGIT and biotinylated TIGIT antibodies used in experimental research to monitor immune responses.
- Biological function summary
TIGIT acts as an inhibitory receptor modulating immune responses. It belongs to the CD28 family and binds to poliovirus receptor (PVR) and nectin-2 on antigen-presenting cells. This interaction inhibits T cell activation and proliferation and increases Treg cell activity maintaining immune homeostasis. TIGIT forms a complex with other inhibitory receptors like PD-1 2B4 and CD96 to further regulate immune functions.
- Pathways
The interaction of TIGIT with its ligands significantly affects the immune checkpoint pathway. This involvement regulates T cell and NK cell activity. TIGIT is related to CD226 (DNAM-1) which competes for binding to its ligands influencing cellular signaling pathways. Through these pathways TIGIT signals through the downstream SHP-1 and SHP-2 proteins affecting immune cell signaling and response.
- Associated diseases and disorders
TIGIT has implications in cancer and autoimmune diseases. Its inhibitory function can allow tumor progression by dampening effective anti-tumor immunity. In cancer TIGIT’s activity often pairs with PD-1 both contributing to immune checkpoint blockade resistance necessary for tumor evasion. In autoimmune diseases increased TIGIT expression could result in reduced immune cell attack against self tissues showing its potential to modulate treatment strategies.
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3 product images
Functional Studies - Recombinant rat TIGIT protein (Fc Chimera Active) (ab224633)
Immobilized human Nectin-2, His Tag at 5 μg/ml (100 μl/well) can bind ab224633 with a linear range of 0.039-5 μg/ml.
SDS-PAGE - Recombinant rat TIGIT protein (Fc Chimera Active) (ab224633)
ab224633 analyzed by SDS-PAGE and stained overnight with Coomassie Blue.
Lane 1: Reducing conditions.
Lane 2: Non-reducing conditions.
As a result of glycosylation, the protein migrates as 48-55 kDa under reducing conditions, and 85-110 kDa under non-reducing conditions.
Functional Studies - Recombinant rat TIGIT protein (Fc Chimera Active) (ab224633)
Loaded ab224633 on Protein A Biosensor, can bind Human Nectin-2, His Tag with an affinity constant of 0.59 μM as determined in BLI assay.
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