JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB226271

Recombinant Renilla Luciferase protein (Tagged)

Be the first to review this product! Submit a review

|

(1 Publication)

Recombinant Renilla Luciferase protein (Tagged) is a Renilla reniformis Full Length protein, in the 1 to 311 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

Coelenterazine h 2-monooxygenase, Renilla-luciferin 2-monooxygenase, Renilla-type luciferase

1 Images
SDS-PAGE - Recombinant Renilla Luciferase protein (Tagged) (AB226271)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Renilla Luciferase protein (Tagged) (AB226271)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

10x His tag N-Terminus SUMO tag N-Terminus Myc tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P27652

Animal free

No

Carrier free

No

Species

Renilla reniformis

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ","proteinLength":"Full Length","predictedMolecularWeight":"56 kDa","actualMolecularWeight":null,"aminoAcidEnd":311,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P27652","tags":[{"tag":"10x His","terminus":"N-Terminus"},{"tag":"SUMO","terminus":"N-Terminus"},{"tag":"Myc","terminus":"C-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Renilla Luciferase also known as RLuc is an enzyme that catalyzes the oxidation of its substrate coelenterazine producing bioluminescence. With a molecular mass of approximately 36 kDa RLuc facilitates this reaction by oxygenation resulting in the emission of blue light. Renilla Luciferase is typically isolated from the sea pansy Renilla reniformis and has been widely used as a reporter gene in molecular biology for monitoring gene expression and cellular events due to its efficient light production.
Biological function summary

Renilla Luciferase operates as part of the bioluminescent system found in certain marine organisms. In its native environment it interacts with other proteins like Green Fluorescent Protein (GFP) to which it transfers energy converting blue light to green fluorescence. This process of bioluminescence serves various ecological functions including predator avoidance and communication between organisms. These interactions highlight the importance of Renilla Luciferase in understanding biological luminescence systems.

Pathways

Researchers use Renilla Luciferase extensively in signaling and metabolic pathway analyses. It plays an important role in the study of G-protein coupled receptor (GPCR) pathways where it can help to analyze receptor activation and downstream communication. In these pathways it often pairs with other luminescent or fluorescent markers like Firefly Luciferase or GFP enabling simultaneous dual-reporter assays to provide more comprehensive data on cellular responses.

Renilla Luciferase aids in the study of cancer and infectious diseases. Its role in reporter assays facilitates the investigation of gene expression changes and the screening of potential therapeutic compounds. For example in cancer research it is frequently paired with p53 proteins for studying apoptosis and cell cycle regulation. Similarly in infectious disease studies Renilla Luciferase reporters can track viral infections and the efficacy of antiviral drugs. These applications highlight its value in advancing biomedical research and drug development.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO(2) molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.

style
left-column

Product protocols

style
left-column

Target data

Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO(2) molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.
See full target information Coelenterazine h 2-monooxygenase
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 9:7075-7087 PubMed29467951

2018

Reverting iodine avidity of radioactive-iodine refractory thyroid cancer with a new tyrosine kinase inhibitor (K905-0266) excavated by high-throughput NIS (sodium iodide symporter) enhancer screening platform using dual reporter gene system.

Applications

WB

Species

Unspecified reactive species

Ji Min Oh,Senthilkumar Kalimuthu,Prakash Gangadaran,Se Hwan Baek,Liya Zhu,Ho Won Lee,Ramya Lakshmi Rajendran,Chae Moon Hong,Shin Young Jeong,Sang-Woo Lee,Jaetae Lee,Byeong-Cheol Ahn
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Proteins & Peptides

AB100961

Recombinant Firefly Luciferase protein

SDS-PAGE - Recombinant Firefly Luciferase protein (AB100961)

  • 0
  • 0
Primary Antibodies

AB185926

Anti-Renilla Luciferase antibody [EPR17792]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Renilla Luciferase antibody [EPR17792] (AB185926)

  • 5
  • 1
Proteins & Peptides

AB318258

Recombinant human PKM2 protein (His tag)

Mass Spectrometry - Recombinant human PKM2 protein (His tag) (AB318258)

  • 0
  • 0
Proteins & Peptides

AB273530

Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag)

ELISA - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com