Recombinant Ribosome inactivating protein alpha trichosanthin (Tagged) is a Gua Lou Full Length protein, in the 24 to 270 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
D V S F R L S G A T S S S Y G V F I S N L R K A L P N E R K L Y D I P L L R S S L P G S Q R Y A L I H L T N Y A D E T I S V A I D V T N V Y I M G Y R A G D T S Y F F N E A S A T E A A K Y V F K D A M R K V T L P Y S G N Y E R L Q T A A G K I R E N I P L G L P A L D S A I T T L F Y Y N A N S A A S A L M V L I Q S T S E A A R Y K F I E Q Q I G K R V D K T F L P S L A I I S L E N S W S A L S K Q I Q I A S T N N G Q F E S P V V L I N A Q N Q R V T I T N V D A G V V T S N I A L L L N R N N M A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Inactivates eukaryotic 60S ribosomal subunits.
Alternative names
Ribosome-inactivating protein alpha-trichosanthin, Alpha-TCS, rRNA N-glycosidase
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Recombinant Ribosome inactivating protein alpha trichosanthin (Tagged) is a Gua Lou Full Length protein, in the 24 to 270 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P09989-1
- Animal free
- No
- Species
- Gua Lou
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- D V S F R L S G A T S S S Y G V F I S N L R K A L P N E R K L Y D I P L L R S S L P G S Q R Y A L I H L T N Y A D E T I S V A I D V T N V Y I M G Y R A G D T S Y F F N E A S A T E A A K Y V F K D A M R K V T L P Y S G N Y E R L Q T A A G K I R E N I P L G L P A L D S A I T T L F Y Y N A N S A A S A L M V L I Q S T S E A A R Y K F I E Q Q I G K R V D K T F L P S L A I I S L E N S W S A L S K Q I Q I A S T N N G Q F E S P V V L I N A Q N Q R V T I T N V D A G V V T S N I A L L L N R N N M A
- Accession
- P09989
- Protein length
- Full Length
- Predicted molecular weight
- 43.1 kDa
- Amino acids
- 24 to 270
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Inactivates eukaryotic 60S ribosomal subunits.
- Sequence similarities
Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ribosome inactivating protein alpha trichosanthin also known as TCS is a type 1 ribosome-inactivating protein (RIP) that disrupts protein synthesis by depurinating adenine residues in ribosomal RNA. This action inhibits the assembly of the ribosome effectively halting protein synthesis. Trichosanthin exhibits a molecular weight of approximately 27 kDa. It is primarily expressed in the plant Trichosanthes kirilowii which belongs to the Cucurbitaceae family.
- Biological function summary
Ribosome-inactivating proteins like trichosanthin possess a range of activities beyond protein synthesis inhibition. They participate in antiviral responses and have been investigated for their potential to target specific cellular processes in cancer cells. Trichosanthin does not function as part of a multimeric complex; rather it acts individually to exert its effects on cell machinery making it a subject of interest in cancer and virology research.
- Pathways
Trichosanthin impacts cellular pathways that involve protein translation and antiviral defense mechanisms. Within these pathways it associates with cellular stress responses and modulates apoptosis. Trichosanthin is related to other ribosome-inactivating proteins such as ricin and saporin that share mechanistic similarities. These proteins interact with ribosomal components to trigger similar downstream effects and influence cellular homeostasis.
- Associated diseases and disorders
Ribosome inactivating protein alpha trichosanthin displays potential therapeutic effects against certain cancers and viral infections. It has shown promising results in research targeting HIV replication by impairing the virus's ability to hijack host cell machinery. Additionally it may be explored for its roles in modulating pathways involved in certain cancers. The protein's connection with other ribosome-inactivating proteins involves shared potential pathways for therapeutic intervention in diseases such as leukemia.
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1 product image
SDS-PAGE - Recombinant Ribosome inactivating protein alpha trichosanthin (Tagged) (ab236919)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel analysis of ab236919.
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