Recombinant RNase A

Product details

Ensure the validity of your result using our recombinant RNase A protein ab300192 to remove RNA contaminants that could interfere with protein or enzyme activity assays.
The bioactive ab300192 enzyme is ideal for applications such as RNA preparation and analysis.

Recommended concentration of RNase A ab300192 is 1 to 100 µg/mL depending on the application.

Extinction Coefficient (ε)1% at 280 nm = 7.1

Function

RNase A (previously sold as Biovision's M1517) is an endoribonuclease that specifically cleaves RNA at the phosphodiester bond between the 3’ phosphate group of a pyrimidine nucleotide and 5’-ribose of an adjacent nucleotide.

The highest activity is demonstrated with single stranded RNA. The recombinant enzyme is identical to the native RNase A in amino acid sequence, structure and specifications.

At low salt concentrations (0 to 100 mM NaCl), RNase A cleaves single-stranded and double-stranded RNA as well the RNA strand in RNA-DNA hybrids. However, at NaCl concentrations of 0.3 M or higher, RNase A specifically cleaves single-stranded RNA.

Precipitation may occur at high concentrations (>10 mg/mL) of the enzyme and RNase A has a high affinity to glass surfaces.

The enzyme is inhibited by diethyl pyrocarbonate (DEPC), guanidinium salts, β-mercaptoethanol, heavy metals and RNase-inhibitors.

In order to remove the enzyme from a sample, perform a separation with spin columns or several phenol/chloroform extractions.

Special care and precautions should be taken in the lab with this enzyme to ensure cross contamination with RNA work does not occur.