AB96554

Recombinant S. cerevisiae KEX2 protease protein (Active)

Bioactive
What is this?

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(1 Publication)

Recombinant S. cerevisiae KEX2 protease protein (Active) is a Saccharomyces cerevisiae S288C Fragment protein, in the 110 to 667 aa range, expressed in BTI-TN-5B1-4 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC, FuncS.

View Alternative Names

QDS1, YNL238W, N1122, KEX2, Kexin, Protease KEX2, Proteinase YSCF

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

BTI-TN-5B1-4 cells

Applications

FuncS, HPLC, SDS-PAGE

applications

Biologically active

Yes

Biological activity

ab96554 from High-5 insect cells contains the same specific activity and recognition sequence specificity as yeast derived KEX2.

1 milligram of recombinant KEX2 contains activity equivalent to at least 40 units of yeast derived KEX2.

Cleaves at the carboxyl side of K/R-R.

Accession

P13134

Animal free

Carrier free

Species

Saccharomyces cerevisiae S288C

Reconstitution

reconstitute with water at 1mg/mL

Storage buffer

Constituents: 0.082% Sodium acetate, 0.0555% Calcium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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Sequence info

[{"sequence":"LPVPAPPMDSSLLPVKEAEDKLSINDPLFERQWHLVNPSFPGSDINVLDLWYNNITGAGVVAAIVDDGLDYENEDLKDNFCAEGSWDFNDNTNLPKPRLSDDYHGTRCAGEIAAKKGNNFCGVGVGYNAKISGIRILSGDITTEDEAASLIYGLDVNDIYSCSWGPADDGRHLQGPSDLVKKALVKGVTEGRDSKGAIYVFASGNGGTRGDNCNYDGYTNSIYSITIGAIDHKDLHPPYSEGCSAVMAVTYSSGSGEYIHSSDINGRCSNSHGGTSAAAPLAAGVYTLLLEANPNLTWRDVQYLSILSAVGLEKNADGDWRDSAMGKKYSHRYGFGKIDAHKLIEMSKTWENVNAQTWFYLPTLYVSQSTNSTEETLESVITISEKSLQDANFKRIEHVTVTVDIDTEIRGTTTVDLISPAGIISNLGVVRPRDVSSEGFKDWTFMSVAHWGENGVGDWKIKVKTTENGHRIDFHSWRLKLFGESIDSSKTETFVFGNDKEEVEPAATESTVSQYSASSTSISISATSTSSISIGVETSAIPQTTTASTDPDSDPNTP","proteinLength":"Fragment","predictedMolecularWeight":"60.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":667,"aminoAcidStart":110,"nature":"Recombinant","expressionSystem":"BTI-TN-5B1-4 cells","accessionNumber":"P13134","tags":[]}]

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

True

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Specifications

Form

Lyophilized

Additional notes

>= 95% HPLC analyses.

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General info

Function

Processing of precursors of alpha-factors and killer toxin.

Sequence similarities

Belongs to the peptidase S8 family. Furin subfamily.

Post-translational modifications

O-glycosylated.

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

Processing of precursors of alpha-factors and killer toxin.

See full target information KEX2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The New phytologist 231:2282-2296 PubMed34053091

2021

The crystal structure of SnTox3 from the necrotrophic fungus Parastagonospora nodorum reveals a unique effector fold and provides insight into Snn3 recognition and pro-domain protease processing of fungal effectors.

Applications

Unspecified application

Species

Unspecified reactive species

Megan A Outram,Yi-Chang Sung,Daniel Yu,Bayantes Dagvadorj,Sharmin A Rima,David A Jones,Daniel J Ericsson,Jana Sperschneider,Peter S Solomon,Bostjan Kobe,Simon J Williams

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Recombinant S. cerevisiae KEX2 protease protein (Active)

Key facts

Purity

Endotoxin level

Expression system

Tags

Applications

Biologically active

Biological activity

Accession

Animal free

Carrier free

Species

Reconstitution

Storage buffer

Reactivity data

Sequence info

Properties and storage information

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Specifications

Form

Additional notes

General info

Function

Sequence similarities

Post-translational modifications

Product protocols

Target data

Publications (1)

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Product promise