Recombinant S. mansoni Major egg antigen protein (His tag) is a Schistosoma mansoni Full Length protein, in the 1 to 354 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M S G G K Q H N A V S I P V N R E Q R S F E K Q R R D L L T G L E H G G G A H R G N S I A P Y T E D W P S T V D N W I D S S W K R W D D D M R R L R R G M F A L L P L D T F S I G I L E N P F A L M H Q M D R Q I Q D I R E R M G S L D V P S T G S V N D F L K D A Y E V G E D G K V H F K V R F D A Q G F A P Q D I N V T S S E N R V T V H A K K E T T T D G R K C S R E F C R M V Q L P K S I D D S Q L K C R M T D D G V L M L E A P V K V D Q N Q S L T L N E S G Q V A V R P K S D N Q I K A V P A S Q A L V A K G V H G L S Y V D D G S G G K R L H V E V A V D P V Y K P E D L F V N V D S N R V V V S G R H H K Q K S D Q H G R S S S F A E F S Q S Y A I P E T V D P L S V S A Q V V G N T L V L E A P L E K Q H A I T H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Alternative names
Major egg antigen, p40
Recommended products
Recombinant S. mansoni Major egg antigen protein (His tag) is a Schistosoma mansoni Full Length protein, in the 1 to 354 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P12812-1
- Animal free
- No
- Species
- Schistosoma mansoni
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- M S G G K Q H N A V S I P V N R E Q R S F E K Q R R D L L T G L E H G G G A H R G N S I A P Y T E D W P S T V D N W I D S S W K R W D D D M R R L R R G M F A L L P L D T F S I G I L E N P F A L M H Q M D R Q I Q D I R E R M G S L D V P S T G S V N D F L K D A Y E V G E D G K V H F K V R F D A Q G F A P Q D I N V T S S E N R V T V H A K K E T T T D G R K C S R E F C R M V Q L P K S I D D S Q L K C R M T D D G V L M L E A P V K V D Q N Q S L T L N E S G Q V A V R P K S D N Q I K A V P A S Q A L V A K G V H G L S Y V D D G S G G K R L H V E V A V D P V Y K P E D L F V N V D S N R V V V S G R H H K Q K S D Q H G R S S S F A E F S Q S Y A I P E T V D P L S V S A Q V V G N T L V L E A P L E K Q H A I T H
- Accession
- P12812
- Protein length
- Full Length
- Predicted molecular weight
- 43.4 kDa
- Amino acids
- 1 to 354
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Sequence similarities
Belongs to the small heat shock protein (HSP20) family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Major Egg Antigen also known as SEA or Schistosoma Egg Antigen is a glycoprotein with an estimated molecular mass of approximately 31-98 kDa depending on the glycosylation state. It is expressed within the eggs of the parasitic flatworm Schistosoma species. These parasites reside in the host's blood vessels and the antigen is significant during the egg-laying stage. It has a role in stimulating host immune responses as it interacts with immune cells.
- Biological function summary
Egg-derived proteins activate and modulate the host immune system. SEA acts as an immunogenic component that influences the balance between pro-inflammatory and anti-inflammatory responses. The antigen comes from eggs in host tissues and induces granuloma formation by promoting immune cell recruitment to infected sites. Although not part of a larger complex its interactions with host immune proteins highlight its importance in disease progression due to its ability to induce a profound immune response.
- Pathways
This antigen affects the immune signaling and regulatory pathways involving cytokines and immune cell receptors. Two important pathways include the T-helper 2 (Th2) cell pathway and the granulomatous inflammation pathway. SEA molecules influence the differentiation of naïve T-cells towards a Th2 response with the involvement of proteins like IL-4 and IL-13. SEA interacts with components of the host's immune response through these pathways affecting the overall modulation of immune activity.
- Associated diseases and disorders
Schistosomiasis is the primary condition connected with SEA. Granuloma formation around eggs lodged in tissues promoted by SEA can lead to fibrosis and organ dysfunction particularly in the liver and intestines. Its interaction with host immune proteins such as interleukins and tumor necrosis factor-alpha exacerbates inflammation. The dysregulation of these immune responses due to SEA aggravates disease manifestations in affected hosts.
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1 product image
SDS-PAGE - Recombinant S. mansoni Major egg antigen protein (His tag) (ab224849)
Discontinuous SDS-PAGE (Tris-Glycine gel) (reduced) analysis of ab224849 with 5% enrichment gel and 15% separation gel.
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