Recombinant Salmonella heidelberg D-serine dehydratase (dsdA) protein (His tag) is a Salmonella enterica subsp. enterica serovar Heidelberg str. SL476 Full Length protein, in the 1 to 440 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
Amino acid sequence
M E N I Q K L I A R Y P L V E D L V A L K E T T W F N P G A T S L A Q G L P Y V G L T E Q D V N A A H D R L A R F A P Y L A K A F P Q T A A A G G M I E S D V V A I P A M Q K R L E K E Y G Q T I D G E M L L K K D S H L A I S G S I K A R G G I Y E V L T H A E K L A L E A G L L T T D D D Y S V L L S P E F K Q F F S Q Y S I A V G S T G N L G L S I G I M S A C I G F K V T V H M S A D A R A W K K A K L R S H G V T V V E Y E D D Y G V A V E Q G R K A A Q S D P N C F F I D D E N S R T L F L G Y A V A G Q R L K A Q F A Q Q G R V V D A S H P L F V Y L P C G V G G G P G G V A F G L K L A F G D N V H C F F A E P T H S P C M L L G V Y T G L H D A I S V Q D I G I D N L T A A D G L A V G R A S G F V G R A M E R L L D G L Y T L D D Q T M Y D M L G W L A Q E E G I R L E P S A L A G M A G P Q R I C A A A A Y Q Q R H G F S Q T Q L G N A T H L V W A T G G G M V P E D E M E Q Y L A K G R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Alternative names
D-serine dehydratase, D-serine deaminase, DSD, dsdA, SeHA_C4132
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Recombinant Salmonella heidelberg D-serine dehydratase (dsdA) protein (His tag) is a Salmonella enterica subsp. enterica serovar Heidelberg str. SL476 Full Length protein, in the 1 to 440 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
- Accession
- B4TA53-1
- Animal free
No
- Species
Salmonella enterica subsp. enterica serovar Heidelberg str. SL476
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
M E N I Q K L I A R Y P L V E D L V A L K E T T W F N P G A T S L A Q G L P Y V G L T E Q D V N A A H D R L A R F A P Y L A K A F P Q T A A A G G M I E S D V V A I P A M Q K R L E K E Y G Q T I D G E M L L K K D S H L A I S G S I K A R G G I Y E V L T H A E K L A L E A G L L T T D D D Y S V L L S P E F K Q F F S Q Y S I A V G S T G N L G L S I G I M S A C I G F K V T V H M S A D A R A W K K A K L R S H G V T V V E Y E D D Y G V A V E Q G R K A A Q S D P N C F F I D D E N S R T L F L G Y A V A G Q R L K A Q F A Q Q G R V V D A S H P L F V Y L P C G V G G G P G G V A F G L K L A F G D N V H C F F A E P T H S P C M L L G V Y T G L H D A I S V Q D I G I D N L T A A D G L A V G R A S G F V G R A M E R L L D G L Y T L D D Q T M Y D M L G W L A Q E E G I R L E P S A L A G M A G P Q R I C A A A A Y Q Q R H G F S Q T Q L G N A T H L V W A T G G G M V P E D E M E Q Y L A K G R
- Accession
- Protein length
Full Length
- Predicted molecular weight
63.3 kDa
- Amino acids
1 to 440
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Sequence similarities
Belongs to the serine/threonine dehydratase family. DsdA subfamily.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Salmonella heidelberg D-serine dehydratase (dsdA) is an enzyme that catalyzes the conversion of D-serine into pyruvate and ammonia. This enzyme plays a vital role in the metabolism of amino acids. It is known as D-serine ammonia-lyase in some literature and has a molecular weight of approximately 50 kDa. D-serine dehydratase is expressed in the cytoplasm of Salmonella heidelberg cells facilitating the organism's use of D-serine as a carbon source which is important for its survival in different environments.
- Biological function summary
D-serine dehydratase enables Salmonella heidelberg to metabolize D-serine efficiently converting it to pyruvate and ammonia. This metabolic ability allows the bacterium to exploit D-serine in nutrient-limited settings. The enzyme operates as a single unit and is not part of a larger protein complex. By breaking down D-serine the bacterium gains a competitive advantage when colonizing the gut environment where amino acids can be scarce.
- Pathways
D-serine dehydratase is integral to amino acid metabolism and the tricarboxylic acid (TCA) cycle. The pyruvate generated from D-serine enters the TCA cycle contributing to energy production and biosynthetic processes. This enzyme works closely with other proteins involved in these pathways such as pyruvate dehydrogenase ensuring a steady flow of metabolites through central carbon metabolism pathways important for bacterial growth and proliferation.
- Associated diseases and disorders
Salmonella heidelberg and its D-serine dehydratase are relevant to foodborne illnesses. This bacterium is a known pathogen causing gastroenteritis in humans. The ability to metabolize D-serine enhances its virulence aiding in its survival in the host environment. The interaction of dsdA with other virulence factors such as Salmonella pathogenicity islands further strengthens its association with disease. Understanding this connection offers potential targets for therapeutic intervention to mitigate Salmonella-related infections.
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3 product images
SDS-PAGE - Recombinant Salmonella heidelberg D-serine dehydratase (dsdA) protein (His tag) (ab225614)
Discontinuous SDS-PAGE (Tris-Glycine gel) (reduced) analysis of ab225614 with 5% enrichment gel and 15% separation gel.
Mass Spectrometry - Recombinant Salmonella heidelberg D-serine dehydratase (dsdA) protein (His tag) (ab225614)
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab225614 could indicate that this peptide derived from E.coli-expressed Salmonella heidelberg (strain SL476) D-serine dehydratase (dsdA).
Mass Spectrometry - Recombinant Salmonella heidelberg D-serine dehydratase (dsdA) protein (His tag) (ab225614)
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab225614 could indicate that this peptide derived from E.coli-expressed Salmonella heidelberg (strain SL476) D-serine dehydratase (dsdA).
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