Recombinant SARS Nucleocapsid Protein (Coronavirus) is a SARS-CoV Full Length protein, in the 16 to 437 aa range, expressed in Escherichia coli, with >95% purity, < 0.1 EU/µg endotoxin level and suitable for SDS-PAGE, WB.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 0.1 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Amino acid sequence
M R G S H H H H H H G M A S H M S D N G P Q S N Q R S A P R I T F G G P T D S T D N N Q N G G R N G A R P K Q R R P Q G L P N N T A S W F T A L T Q H G K E E L R F P R G Q G V P I N T N S G P D D Q I G Y Y R R A T R R V R G G D G K M K E L S P R W Y F Y Y L G T G P E A S L P Y G A N K E G I V W V A T E G A L N T P K D H I G T R N P N N N A A T V L Q L P Q G T T L P K G F Y A E G S R G G S Q A S S R S S S R S R G N S R N S T P G S S R G N S P A R M A S G G G E T A L A L L L L D R L N Q L E S K V S G K G Q Q Q Q G Q T V T K K S A A E A S K K P R Q K R T A T K Q Y N V T Q A F G R R G P E Q T Q G N F G D Q D L I R Q G T D Y K H W P Q I A Q F A P S A S A F F G M S R I G M E V T P S G T W L T Y H G A I K L D D K D P Q F K D N V I L L N K H I D A Y K T F P P T E P K K D K K K K T D E A Q P L P Q R Q K K Q P T V T L L P A A D M D D F S R Q L Q N S M S G A S A D S T Q A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication (PubMed:17210170). May modulate transforming growth factor-beta signaling by binding host SMAD3 (PubMed:18055455).
Additional Targets
SARS Nucleocapsid Protein
Alternative names
9a, N, Nucleoprotein, Nucleocapsid protein, NC, Protein N
Recommended products
Recombinant SARS Nucleocapsid Protein (Coronavirus) is a SARS-CoV Full Length protein, in the 16 to 437 aa range, expressed in Escherichia coli, with >95% purity, < 0.1 EU/µg endotoxin level and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 0.1 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- P59595-1
- Animal free
- No
- Species
- SARS-CoV
- Reconstitution
- Reconstitute at 0.5 mg/mL in 0.1 M acetate buffer, pH 4.0
- Concentration
- Storage buffer
Constituents: 0.082% Sodium acetate
Sequence info
Amino acid sequence
- M R G S H H H H H H G M A S H M S D N G P Q S N Q R S A P R I T F G G P T D S T D N N Q N G G R N G A R P K Q R R P Q G L P N N T A S W F T A L T Q H G K E E L R F P R G Q G V P I N T N S G P D D Q I G Y Y R R A T R R V R G G D G K M K E L S P R W Y F Y Y L G T G P E A S L P Y G A N K E G I V W V A T E G A L N T P K D H I G T R N P N N N A A T V L Q L P Q G T T L P K G F Y A E G S R G G S Q A S S R S S S R S R G N S R N S T P G S S R G N S P A R M A S G G G E T A L A L L L L D R L N Q L E S K V S G K G Q Q Q Q G Q T V T K K S A A E A S K K P R Q K R T A T K Q Y N V T Q A F G R R G P E Q T Q G N F G D Q D L I R Q G T D Y K H W P Q I A Q F A P S A S A F F G M S R I G M E V T P S G T W L T Y H G A I K L D D K D P Q F K D N V I L L N K H I D A Y K T F P P T E P K K D K K K K T D E A Q P L P Q R Q K K Q P T V T L L P A A D M D D F S R Q L Q N S M S G A S A D S T Q A
- Accession
- P59595
- Protein length
- Full Length
- Predicted molecular weight
- 47.8 kDa
- Amino acids
- 16 to 437
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.ab63311 was purifed using Ni-NTA chromatography.
General info
- Function
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication (PubMed:17210170). May modulate transforming growth factor-beta signaling by binding host SMAD3 (PubMed:18055455).
- Sequence similarities
Belongs to the betacoronavirus nucleocapsid protein family.
- Post-translational modifications
Proteolytically cleaved by host CASP6. The cleavage leads to two fragments and facilitates viral replication by inhibiting host IFN signaling. The two fragments may interact with IRF3 inhibiting its nuclear translocation after activation and reduce the expression of IFNB and IFN-stimulated genes.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Reconstituted protein can be stored at 4 °C for a week.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The nucleocapsid protein of SARS-CoV-2 also known as N protein or COVID-19 nucleocapsid protein is important for the viral replication and packaging processes. It has a mass of approximately 43-46 kDa and is expressed in the cytoplasm of infected host cells. This protein is highly conserved among coronaviruses and plays a significant role in the viral lifecycle by binding to the viral RNA genome thereby facilitating its encapsulation within the virion. Moreover it aids in stabilizing the viral RNA structure and is essential for ribonucleoprotein complex formation.
- Biological function summary
The nucleocapsid protein of SARS-CoV-2 interacts directly with other viral components and host cellular machinery to orchestrate efficient viral assembly and release. It forms a ribonucleoprotein complex by self-association and binding to the RNA genome promoting virion integrity. This protein also plays a part in modulating host cell responses potentially by interfering with antiviral signaling pathways ensuring that the virus evades the host immune system to some degree.
- Pathways
Many scientific studies show that the SARS-CoV-2 nucleocapsid protein is involved in the viral replication and assembly pathways. It interacts with viral proteins such as the membrane (M) protein and possibly others to facilitate these processes. The protein also exhibits connectivity with cellular pathways related to RNA processing and metabolism due to its RNA-binding capabilities further influencing viral replication efficiency and host-pathogen interactions.
- Associated diseases and disorders
The nucleocapsid protein has a significant association with COVID-19 the disease caused by SARS-CoV-2. Its expression correlates with viral pathogenicity and immune response dysregulation which can lead to severe outcomes in infected individuals. The N protein has links with comorbid conditions potentially affecting disease progression. Furthermore the nucleocapsid protein interacts with host proteins like G3BP1 and other cellular factors that may contribute to immune modulation influencing the clinical symptoms associated with COVID-19.
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1 product image
SDS-PAGE - Recombinant SARS Nucleocapsid Protein (Coronavirus) (ab63311)
1. MW marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and heated sample, 5μg/lane
3. non-reduced and non-heated sample, 5μg/lane
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