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AB63311

Recombinant SARS Nucleocapsid Protein (Coronavirus)

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Recombinant SARS Nucleocapsid Protein (Coronavirus) is a SARS-CoV Full Length protein, in the 16 to 437 aa range, expressed in Escherichia coli, with >95%, < 0.1 EU/µg endotoxin level, suitable for SDS-PAGE, WB.

View Alternative Names

9a, N, Nucleoprotein, Nucleocapsid protein, NC, Protein N, 2019-nCoV nucleocapsid protein, NC, Nucleocapsid protein, Nucleoprotein, Protein N, SARS CoV, SARS coronavirus N protein

1 Images
SDS-PAGE - Recombinant SARS Nucleocapsid Protein (Coronavirus) (AB63311)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant SARS Nucleocapsid Protein (Coronavirus) (AB63311)

1. MW marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and heated sample, 5μg/lane
3. non-reduced and non-heated sample, 5μg/lane

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 0.1 EU/µg

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, WB

applications

Biologically active

No

Accession

P59595

Animal free

No

Carrier free

No

Species

SARS-CoV

Reconstitution

Reconstitute at 0.5 mg/mL in 0.1 M acetate buffer, pH 4.0

Storage buffer

Constituents: 0.082% Sodium acetate

storage-buffer

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Complementary Products

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Proteins & Peptides

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Secondary Antibodies

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Primary Antibodies

AB271180

Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (AB271180)

  • 5
  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Reconstituted protein can be stored at 4 °C for a week.
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Sequence info

[{"sequence":"MRGSHHHHHHGMASHMSDNGPQSNQRSAPRITFGGPTDSTDNNQNGGRNGARPKQRRPQGLPNNTASWFTALTQHGKEELRFPRGQGVPINTNSGPDDQIGYYRRATRRVRGGDGKMKELSPRWYFYYLGTGPEASLPYGANKEGIVWVATEGALNTPKDHIGTRNPNNNAATVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRGNSRNSTPGSSRGNSPARMASGGGETALALLLLDRLNQLESKVSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKQYNVTQAFGRRGPEQTQGNFGDQDLIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYHGAIKLDDKDPQFKDNVILLNKHIDAYKTFPPTEPKKDKKKKTDEAQPLPQRQKKQPTVTLLPAADMDDFSRQLQNSMSGASADSTQA","proteinLength":"Full Length","predictedMolecularWeight":"47.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":437,"aminoAcidStart":16,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P59595","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The nucleocapsid protein of SARS-CoV-2 also known as N protein or COVID-19 nucleocapsid protein is important for the viral replication and packaging processes. It has a mass of approximately 43-46 kDa and is expressed in the cytoplasm of infected host cells. This protein is highly conserved among coronaviruses and plays a significant role in the viral lifecycle by binding to the viral RNA genome thereby facilitating its encapsulation within the virion. Moreover it aids in stabilizing the viral RNA structure and is essential for ribonucleoprotein complex formation.
Biological function summary

The nucleocapsid protein of SARS-CoV-2 interacts directly with other viral components and host cellular machinery to orchestrate efficient viral assembly and release. It forms a ribonucleoprotein complex by self-association and binding to the RNA genome promoting virion integrity. This protein also plays a part in modulating host cell responses potentially by interfering with antiviral signaling pathways ensuring that the virus evades the host immune system to some degree.

Pathways

Many scientific studies show that the SARS-CoV-2 nucleocapsid protein is involved in the viral replication and assembly pathways. It interacts with viral proteins such as the membrane (M) protein and possibly others to facilitate these processes. The protein also exhibits connectivity with cellular pathways related to RNA processing and metabolism due to its RNA-binding capabilities further influencing viral replication efficiency and host-pathogen interactions.

The nucleocapsid protein has a significant association with COVID-19 the disease caused by SARS-CoV-2. Its expression correlates with viral pathogenicity and immune response dysregulation which can lead to severe outcomes in infected individuals. The N protein has links with comorbid conditions potentially affecting disease progression. Furthermore the nucleocapsid protein interacts with host proteins like G3BP1 and other cellular factors that may contribute to immune modulation influencing the clinical symptoms associated with COVID-19.
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Specifications

Form

Lyophilized

Additional notes

Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.ab63311 was purifed using Ni-NTA chromatography.

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General info

Function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication (PubMed : 17210170). May modulate transforming growth factor-beta signaling by binding host SMAD3 (PubMed : 18055455).

Sequence similarities

Belongs to the betacoronavirus nucleocapsid protein family.

Post-translational modifications

Proteolytically cleaved by host CASP6. The cleavage leads to two fragments and facilitates viral replication by inhibiting host IFN signaling. The two fragments may interact with IRF3 inhibiting its nuclear translocation after activation and reduce the expression of IFNB and IFN-stimulated genes.. ADP-ribosylated. The ADP-ribosylation is retained in the virion during infection.. Phosphorylated on serine and threonine residues (PubMed:19106108). Phosphorylated by host GSK3A and GSK3B. Phosphorylation allows recruitment of host RNA helicase DDX1 which facilitates template readthrough and enables longer subgenomic mRNA synthesis. This promotes the solubility of homodimers that would otherwise aggregate (PubMed:32974389). Host phosphatase would dephosphorylate the protein during assembly at M bound membranes (PubMed:32974389).

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Product protocols

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Target data

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication (PubMed : 17210170). May modulate transforming growth factor-beta signaling by binding host SMAD3 (PubMed : 18055455).
See full target information N

Additional targets

SARS Nucleocapsid Protein
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