Recombinant Severe Fever with Thrombocytopenia Syndrome Virus Nucleoprotein (His tag)

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleoprotein also known as NP is a structural protein that plays an important role in the assembly and genomic encapsidation of several viruses including influenza and hantavirus. It has a molecular mass that varies depending on the specific virus but typically ranges from 50 to 60 kDa. Nucleoprotein is expressed predominantly within the nuclear and cytoplasmic regions of infected host cells. It interacts directly with the viral RNA forming a ribonucleoprotein complex that facilitates the viral replication process.

Biological function summary

Nucleoprotein acts as an essential component in the viral lifecycle by aiding in the protection and packaging of viral RNA. It typically associates with other viral proteins to form a ribonucleoprotein complex which is necessary for viral replication and transcription. This complex ensures the encapsidation of the viral genome protecting it from nucleases and allowing proper assembly into new virions. Additionally nucleoprotein has been found to modulate host immune responses potentially aiding viral evasion of host defense mechanisms.

Pathways

Nucleoprotein participates in viral replication and transcription pathways particularly within the context of the host-virus interaction. It is associated with the replication machinery of the virus where it interacts with the polymerase complex which includes proteins such as polymerase basic 1 (PB1) and polymerase basic 2 (PB2). This interaction facilitates the synthesis of complementary RNA strands necessary for viral propagation and the production of viral proteins.

Nucleoprotein’s involvement is significant in conditions like viral hemorrhagic fevers such as those caused by hantavirus which lead to fever and thrombocytopenia. It plays a role in diseases by contributing to viral pathogenesis and influencing disease severity. The interaction of nucleoprotein with other proteins such as glycoproteins found in the viral envelope highlights its importance in the infectious cycle and its potential as a target for therapeutic interventions and vaccine development.