Recombinant Simian Virus 40 Major Capsid VP1 protein is a Simian Virus 40 Full Length protein, expressed in Saccharomyces cerevisiae, with >85% purity and suitable for ELISA, WB, SDS-PAGE.
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- Nature nanotechnology 18:1205-12122023DNA-origami-directed virus capsid polymorphism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIris Seitz,Sharon Saarinen,Esa-Pekka Kumpula,Donna McNeale,Eduardo Anaya-Plaza,Vili Lampinen,Vesa P Hytönen,Frank Sainsbury,Jeroen J L M Cornelissen,Veikko Linko,Juha T Huiskonen,Mauri A KostiainenPubMed 37460794
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Saccharomyces cerevisiae
- Tags
- Tag free
- Applications
- ELISA, WB, SDS-PAGE
- Biologically active
- No
Amino acid sequence
M K M A P T K R K G S C P G A A P K K P K E P V Q V P K L V I K G G I E V L G V K T G V D S F T E V E C F L N P Q M G N P D E H Q K G L S K S L A A E K Q F T D D S P D K E Q L P C Y S V A R I P L P N L N E D L T C G N I L M W E A V T V K T E V I G V T A M L N L H S G T Q K T H E N G A G K P I Q G S N F H F F A V G G E P L E L Q G V L A N Y R T K Y P A Q T V T P K N A T V D S Q Q M N T D H K A V L D K D N A Y P V E C W V P D P S K N E N T R Y F G T Y T G G E N V P P V L H I T N T A T T V L L D E Q G V G P L C K A D S L Y V S A V D I C G L F T N T S G T Q Q W K G L P R Y F K I T L R K R S V K N P Y P I S F L L S D L I N R R T Q R V D G Q P M I G M S S Q V E E V R V Y E D T E E L P G D P D M I R Y I D E F G Q T T T R L Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info 0.50000-5.00000 µg/mL | Notes - |
Application WB | Reactivity Reacts | Dilution info 1.00000-5.00000 µg/mL | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info 4.2 µg/mL | Notes - |
Target data
Function
Forms an icosahedral capsid with a T=7 symmetry and a 40 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with VP2 or VP3 proteins. Binds to N-glycolylneuraminic analog of the ganglioside GM1 on the cell surface to provide virion attachment to target cell (PubMed:18353982). Once attached, the virion is internalized by caveolin-mediated endocytosis and traffics to the endoplasmic reticulum. Inside the endoplasmic reticulum, the protein folding machinery isomerizes VP1 interpentamer disulfide bonds, thereby triggering initial uncoating (PubMed:17981119). Next, the virion uses the endoplasmic reticulum-associated degradation machinery to probably translocate in the cytosol before reaching the nucleus (PubMed:17981119). Nuclear entry of the viral DNA involves the selective exposure and importin recognition of VP2/Vp3 nuclear localization signal. The assembly takes place in the cell nucleus. Encapsulates the genomic DNA and participates in rearranging nucleosomes around the viral DNA. The viral progenies exit the cells by lytic release.
Additional Targets
Simian Virus 40 Major Capsid VP1
Alternative names
Major capsid protein VP1, Major structural protein VP1
Recommended products
Recombinant Simian Virus 40 Major Capsid VP1 protein is a Simian Virus 40 Full Length protein, expressed in Saccharomyces cerevisiae, with >85% purity and suitable for ELISA, WB, SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Saccharomyces cerevisiae
- Tags
- Tag free
- Applications
- ELISA, WB, SDS-PAGE
- Biologically active
- No
- Accession
- P03087-1
- Animal free
- No
- Species
- Simian Virus 40
- Reconstitution
- Reconstitute in water
- Concentration
- Storage buffer
Constituents: PBS
Sequence info
Amino acid sequence
- M K M A P T K R K G S C P G A A P K K P K E P V Q V P K L V I K G G I E V L G V K T G V D S F T E V E C F L N P Q M G N P D E H Q K G L S K S L A A E K Q F T D D S P D K E Q L P C Y S V A R I P L P N L N E D L T C G N I L M W E A V T V K T E V I G V T A M L N L H S G T Q K T H E N G A G K P I Q G S N F H F F A V G G E P L E L Q G V L A N Y R T K Y P A Q T V T P K N A T V D S Q Q M N T D H K A V L D K D N A Y P V E C W V P D P S K N E N T R Y F G T Y T G G E N V P P V L H I T N T A T T V L L D E Q G V G P L C K A D S L Y V S A V D I C G L F T N T S G T Q Q W K G L P R Y F K I T L R K R S V K N P Y P I S F L L S D L I N R R T Q R V D G Q P M I G M S S Q V E E V R V Y E D T E E L P G D P D M I R Y I D E F G Q T T T R L Q
- Accession
- P03087
- Protein length
- Full Length
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Purified by ultracentifugation
General info
- Function
Forms an icosahedral capsid with a T=7 symmetry and a 40 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with VP2 or VP3 proteins. Binds to N-glycolylneuraminic analog of the ganglioside GM1 on the cell surface to provide virion attachment to target cell (PubMed:18353982). Once attached, the virion is internalized by caveolin-mediated endocytosis and traffics to the endoplasmic reticulum. Inside the endoplasmic reticulum, the protein folding machinery isomerizes VP1 interpentamer disulfide bonds, thereby triggering initial uncoating (PubMed:17981119). Next, the virion uses the endoplasmic reticulum-associated degradation machinery to probably translocate in the cytosol before reaching the nucleus (PubMed:17981119). Nuclear entry of the viral DNA involves the selective exposure and importin recognition of VP2/Vp3 nuclear localization signal. The assembly takes place in the cell nucleus. Encapsulates the genomic DNA and participates in rearranging nucleosomes around the viral DNA. The viral progenies exit the cells by lytic release.
- Sequence similarities
Belongs to the polyomaviruses coat protein VP1 family.
- Subcellular localisation
- Host nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
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1 product image
SDS-PAGE - Recombinant Simian Virus 40 Major Capsid VP1 protein (ab74565)
SDS-PAGE showing ab74565 at approximately 44kDa (4.2μg/lane)
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