Recombinant Simian Virus 40 SV40 Large T Antigen protein is a Simian Virus 40 Full Length protein, in the 1 to 708 aa range, expressed in Baculovirus, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE. The predicted molecular weight of ab82118 protein is 83.3 kDa.
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Publications
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- Molecular medicine reports 16:8819-88252017Ultrasound‑targeted microbubbles combined with a peptide nucleic acid binding nuclear localization signal mediate transfection of exogenous genes by improving cytoplasmic and nuclear import.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNan Jiang et. al.PubMed 28990051
Key facts
- Purity
- >95%
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M D K V L N R E E S L Q L M D L L G L E R S A W G N I P L M R K A Y L K K C K E F H P D K G G D E E K M K K M N T L Y K K M E D G V K Y A H Q P D F G G F W D A T E I P T Y G T D E W E Q W W N A F N E E N L F C S E E M P S S D D E A T A D S Q H S T P P K K K R K V E D P K D F P S E L L S F L S H A V F S N R T L A C F A I Y T T K E K A A L L Y K K I M E K Y S V T F I S R H N S Y N H N I L F F L T P H R H R V S A I N N Y A Q K L C T F S F L I C K G V N K E Y L M Y S A L T R D P F S V I E E S L P G G L K E H D F N P E E A E E T K Q V S W K L V T E Y A M E T K C D D V L L L L G M Y L E F Q Y S F E M C L K C I K K E Q P S H Y K Y H E K H Y A N A A I F A D S K N Q K T I C Q Q A V D T V L A K K R V D S L Q L T R E Q M L T N R F N D L L D R M D I M F G S T G S A D I E E W M A G V A W L H C L L P K M D S V V Y D F L K C M V Y N I P K K R Y W L F K G P I D S G K T T L A A A L L E L C G G K A L N V N L P L D R L N F E L G V A I D Q F L V V F E D V K G T G G E S R D L P S G Q G I N N L D N L R D Y L D G S V K V N L E K K H L N K R T Q I F P P G I V T M N E F S V P K T L Q A R F V K Q I D F R A K D Y L K H C L E R S E F L L E K R I I Q S G I A L L L M L I W Y R P V A E F A Q S I Q S R I V E W K E R L D K E F S L S V Y Q K M K F N V A M G I G V L D W L R N S D D D D E D S Q E N A D K N E D G G E K N M E D S G H E T G I D S Q S Q G S F Q A P Q S S Q S V H D H N Q P Y H I C R G F T C F K K P P T P P P E P E T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Isoform large T antigen is a key early protein essential for both driving viral replication and inducing cellular transformation. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle and by autoregulating the synthesis of viral early mRNA. Displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. Participates in the modulation of cellular gene expression preceeding viral DNA replication. This step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53. Induces the disassembly of host E2F1 transcription factors from RB1, thus promoting transcriptional activation of E2F1-regulated S-phase genes. Inhibits host TP53 binding to DNA, abrogating the ability of TP53 to stimulate gene expression. Plays the role of a TFIID-associated factor (TAF) in transcription initiation for all three RNA polymerases, by stabilizing the TBP-TFIIA complex on promoters. Initiates viral DNA replication and unwinding via interactions with the viral origin of replication. Binds two adjacent sites in the SV40 origin. The replication fork movement is facilitated by Large T antigen helicase activity. Has processive 3'-5' DNA helicase activity which requires a short 3' single-stranded region and ATP; other (d)NTPs can partially replace ATP (PubMed:2826443, PubMed:2826446). Activates the transcription of viral late mRNA, through host TBP and TFIIA stabilization. Interferes with histone deacetylation mediated by HDAC1, leading to activation of transcription. May inactivate the growth-suppressing properties of the E3 ubiquitin ligase CUL7. Isoform 17kT antigen targets host RBL2 for degradation and promotes cell proliferation. Transactivates host cyclin A promoter through its J domain. Unwinds G4 DNA (planar arrays of 4 guanine bases stabilized by hydrogen bonds, parallel and antiparallel arrays were tested); unwinding occurs in the 3'-5' direction, requires a 3' single-stranded end and hydrolyzable ATP (PubMed:9016557).
Additional Targets
SV40 Large T Antigen
Alternative names
Large T antigen, LT, LT-AG, DNA 3'-5' helicase large T antigen
Recommended products
Recombinant Simian Virus 40 SV40 Large T Antigen protein is a Simian Virus 40 Full Length protein, in the 1 to 708 aa range, expressed in Baculovirus, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE. The predicted molecular weight of ab82118 protein is 83.3 kDa.
- Save time and ensure accurate results - use our recombinant SV40 - Antigen protein as a control
Key facts
- Purity
- >95%
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P03070-1
- Animal free
- No
- Species
- Simian Virus 40
- Concentration
- Storage buffer
pH: 7.9
Constituents: 20% Glycerol (glycerin, glycerine), 0.75% Potassium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00584% EDTA
Sequence info
Amino acid sequence
- M D K V L N R E E S L Q L M D L L G L E R S A W G N I P L M R K A Y L K K C K E F H P D K G G D E E K M K K M N T L Y K K M E D G V K Y A H Q P D F G G F W D A T E I P T Y G T D E W E Q W W N A F N E E N L F C S E E M P S S D D E A T A D S Q H S T P P K K K R K V E D P K D F P S E L L S F L S H A V F S N R T L A C F A I Y T T K E K A A L L Y K K I M E K Y S V T F I S R H N S Y N H N I L F F L T P H R H R V S A I N N Y A Q K L C T F S F L I C K G V N K E Y L M Y S A L T R D P F S V I E E S L P G G L K E H D F N P E E A E E T K Q V S W K L V T E Y A M E T K C D D V L L L L G M Y L E F Q Y S F E M C L K C I K K E Q P S H Y K Y H E K H Y A N A A I F A D S K N Q K T I C Q Q A V D T V L A K K R V D S L Q L T R E Q M L T N R F N D L L D R M D I M F G S T G S A D I E E W M A G V A W L H C L L P K M D S V V Y D F L K C M V Y N I P K K R Y W L F K G P I D S G K T T L A A A L L E L C G G K A L N V N L P L D R L N F E L G V A I D Q F L V V F E D V K G T G G E S R D L P S G Q G I N N L D N L R D Y L D G S V K V N L E K K H L N K R T Q I F P P G I V T M N E F S V P K T L Q A R F V K Q I D F R A K D Y L K H C L E R S E F L L E K R I I Q S G I A L L L M L I W Y R P V A E F A Q S I Q S R I V E W K E R L D K E F S L S V Y Q K M K F N V A M G I G V L D W L R N S D D D D E D S Q E N A D K N E D G G E K N M E D S G H E T G I D S Q S Q G S F Q A P Q S S Q S V H D H N Q P Y H I C R G F T C F K K P P T P P P E P E T
- Accession
- P03070
- Protein length
- Full Length
- Predicted molecular weight
- 83.3 kDa
- Actual molecular weight
- 83.3 kDa
- Amino acids
- 1 to 708
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab82118 is greater than 95% homogeneous based on SDS-PAGE analysis, purified by affinity and FPLC chromatography.
General info
- Function
Isoform large T antigen is a key early protein essential for both driving viral replication and inducing cellular transformation. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle and by autoregulating the synthesis of viral early mRNA. Displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. Participates in the modulation of cellular gene expression preceeding viral DNA replication. This step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53. Induces the disassembly of host E2F1 transcription factors from RB1, thus promoting transcriptional activation of E2F1-regulated S-phase genes. Inhibits host TP53 binding to DNA, abrogating the ability of TP53 to stimulate gene expression. Plays the role of a TFIID-associated factor (TAF) in transcription initiation for all three RNA polymerases, by stabilizing the TBP-TFIIA complex on promoters. Initiates viral DNA replication and unwinding via interactions with the viral origin of replication. Binds two adjacent sites in the SV40 origin. The replication fork movement is facilitated by Large T antigen helicase activity. Has processive 3'-5' DNA helicase activity which requires a short 3' single-stranded region and ATP; other (d)NTPs can partially replace ATP (PubMed:2826443, PubMed:2826446). Activates the transcription of viral late mRNA, through host TBP and TFIIA stabilization. Interferes with histone deacetylation mediated by HDAC1, leading to activation of transcription. May inactivate the growth-suppressing properties of the E3 ubiquitin ligase CUL7.
- Post-translational modifications
Phosphorylated on both serine and threonine residues. Phosphorylation on Ser-120 and Ser-123 inhibits viral replication, while phosphorylation on Thr-124 enhances replication by activating the DNA-binding domain. Phosphorylation on Thr-701 is required for binding to host FBW7gamma isoform. Dephosphorylated preferentially by PP2A on Ser-120, Ser-123, Ser-677 and perhaps Ser-679. Small t antigen inhibits the dephosphorylation by the AC form of PP2A.
- Subcellular localisation
- Host nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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SDS-PAGE - Recombinant Simian Virus 40 SV40 Large T Antigen protein (ab82118)
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