JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB236166

Recombinant Soybean RUBISCO protein (Tagged)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Soybean RUBISCO protein (Tagged) is a Soybean Full Length protein, in the 3 to 475 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

Ribulose bisphosphate carboxylase large chain, RuBisCO large subunit, rbcL

1 Images
SDS-PAGE - Recombinant Soybean RUBISCO protein (Tagged) (AB236166)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Soybean RUBISCO protein (Tagged) (AB236166)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab236166.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P27066

Animal free

No

Carrier free

No

Species

Soybean

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Sequence info

[{"sequence":"PQTETKASVGFKAGVKDYKLTYYTPDYETKDTDILAAFRVTPQPGVPPEEAGAAVAAESSTGTWTTVWTDGLTSLDRYKGRCYGLEPVAGEENQYIAYVAYPLDLFEEGSVTNMFTSIVGNVFGFKALRALRLEDLRIPTAYIKTFQGPPHGIQVERDKLNKYGRPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRDRFLFCAEAIFKSQAETGEIKGHYLNATAGTCEEMMKRAVFARELGVPIVMHDYLTGGFTANTSLAHYCRDNGLLLHIHRAMHAVIDRQKNHGMHFRVLAKALRLSGGDHVHAGTVVGKLEGEREITLGFVDLLRDDFVEKDRSRGIYFTQDWVSLPGVLPVASGGIHVWHMPALTEIFGDDSVLQFGGGTLGHPWGNAPGAVANRVALEACVQARNEGRDLAREGNEIIREASKWSPELAAACEVWKEIKFEFEAMDTL","proteinLength":"Full Length","predictedMolecularWeight":"68.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":475,"aminoAcidStart":3,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P27066","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ribulose-15-bisphosphate carboxylase/oxygenase often known as RuBisCO plays an important role in the process of carbon fixation. RuBisCO catalyzes the reaction between carbon dioxide and ribulose-15-bisphosphate a five-carbon sugar resulting in two molecules of 3-phosphoglycerate. The enzyme's mass is about 550 kilodaltons making it one of the most abundant proteins in the world. Expression of RuBisCO predominantly occurs in the chloroplasts of photosynthetic organisms particularly in plants algae and certain bacteria.
Biological function summary

RuBisCO initiates the Calvin Cycle acting as the enzyme that drives the conversion of inorganic carbon dioxide into organic compounds. The protein exists as part of a larger complex comprising eight large subunits and eight small subunits which contribute to its catalytic effectiveness. This function is important for the synthesis of sugars that support plant growth. The enzyme also plays a role in photorespiration by fixing oxygen although this process is less efficient compared to carbon fixation.

Pathways

RuBisCO plays a central role in the carbon fixation pathway of photosynthesis enabling the conversion of light energy into chemical energy gained from carbohydrates. It is critical for the Calvin Cycle and interacts with other proteins such as glyceraldehyde-3-phosphate dehydrogenase during the pathway. Secondly RuBisCO contributes to the photorespiratory pathway though its affinity for oxygen can cause competition with NADP+ reduction during photosynthesis. Interactions with RuBisCO activase are necessary for maintaining its activity under varying conditions.

RuBisCO's efficiency is connected to plant stress responses and adaptability influencing crop yields and food security. Under certain conditions RuBisCO is less effective leading to increased photorespiration and decreased plant productivity especially during drought conditions. Alterations in RuBisCO activity influenced by related proteins like RuBisCO activase can contribute to reduced efficiencies in carbon dioxide fixation impacting plant growth and potential food resources though this is more of an agricultural challenge than a human disease.

Specifications

Form

Liquid

General info

Function

RuBisCO catalyzes two reactions : the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.

Sequence similarities

Belongs to the RuBisCO large chain family. Type I subfamily.

Post-translational modifications

The disulfide bond which can form in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover.

Subcellular localisation

Plastid

Product protocols

Target data

RuBisCO catalyzes two reactions : the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.
See full target information rbcL

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com