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AB60884

Recombinant Src protein

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(5 Publications)

Recombinant Src protein is a Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
4 Images
Functional Studies - Recombinant Src protein (AB60884)
  • FuncS

Unknown

Functional Studies - Recombinant Src protein (AB60884)

The specific activity of Src (ab60884) was determined to be 112 nmol/min/mg as per activity assay protocol

Functional Studies - Recombinant Src protein (AB60884)
  • FuncS

Unknown

Functional Studies - Recombinant Src protein (AB60884)

Sample Kinase Activity Plot.

SDS-PAGE - Recombinant Src protein (AB60884)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Src protein (AB60884)

ab60884 on SDS-PAGE, MW ~85 kDa.

SDS-PAGE - Recombinant Src protein (AB60884)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Src protein (AB60884)

SDS PAGE analysis of ab60884

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

116nmol/min/mg

Animal free

No

Carrier free

No

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab204883 (CDK1 peptide) can be utilized as a substrate for assessing kinase activity
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Sequence info

[{"sequence":"MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSPEFMGSSKSKPKDPSQRRRSLEPPDSTHHGGFPASQTPNKTAAPDTHRTPSRSFGTVATEPKLFGDFNTSDTVTSPQRAGALAGGVTTFVALYDYESWIETDLSFKKGERLQIVNNTEGNWWLAHSVTTGQTGYIPSNYVAPSDSIQAEEWYFGKITRRESERLLLNPENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFSSLQQLVAYYSKHADGLCHRLTNVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVIEYMSKGSLLDFLKGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRMPYPGMGNGEVLDRVERGYRMPCPPECPESLHDLMCQCWRRDPEERPTFEYLQAQLLPACVLEVAE","proteinLength":"Full Length","predictedMolecularWeight":"85 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":null,"tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Src also known as c-Src is a protein-tyrosine kinase involved in the regulation of many cellular processes. The molecular weight of Src is approximately 60 kDa. Src is ubiquitously expressed in human tissues but shows increased expression in specific tissues like the brain and epithelial cells. This protein has several important roles in cellular signal transduction particularly influencing cell growth differentiation and survival.
Biological function summary

The Src protein interacts with other proteins to modulate cell adhesion motility and angiogenesis forming part of larger protein complexes. Src phosphorylates specific tyrosine residues on its substrates altering their activity interaction or stability. This activity positions Src as an important factor in managing cell communication and structural organization. Src's interaction with focal adhesion complexes emphasizes its functionality in cellular structural integrity and intracellular communication pathways.

Pathways

Src plays a critical role in the integrin and growth factor receptor signaling pathways mediating cross-talk between cell surface receptors and intricate signaling cascades. It closely associates with focal adhesion kinase (FAK) within these pathways influencing cytoskeletal rearrangements and cell movement. Src's function in the epidermal growth factor receptor (EGFR) signaling pathway likewise demonstrates its importance in regulating cellular proliferation and survival mechanisms.

Src has significant implications in oncogenesis particularly in colorectal and breast cancers. Overexpression or abnormal activity of Src associates with tumor progression and metastasis. Within these cancers Src cooperates with various proteins like the EGFR amplifying aberrant cell signaling that contributes to uncontrolled cell growth. Investigating Src's role and regulation could offer insights into novel therapeutic strategies for controlling Src activity in cancer treatment.
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Specifications

Form

Liquid

Additional notes

Purity: >90% as determined by densitometry. Affinity purified.

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General info

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Product protocols

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Target data

See full target information SRC
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 20:505-524 PubMed37772772

2023

Direct regulation of FNIP1 and FNIP2 by MEF2 sustains MTORC1 activation and tumor progression in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Li Xia,Tiejian Nie,Fangfang Lu,Lu Huang,Xiaolong Shi,Dongni Ren,Jianjun Lu,Xiaobin Li,Tuo Xu,Bozhou Cui,Qing Wang,Guodong Gao,Qian Yang

Analytical biochemistry 608:113892 PubMed32810472

2020

Electrochemical detection of neuronal extracellular phosphorylation by PKA, PKC and Src.

Applications

Unspecified application

Species

Unspecified reactive species

S Ahmad,M N Hossain,S Ahmadi,K Kerman,H-B Kraatz

Analytical biochemistry 574:46-56 PubMed30914244

2019

Electrochemical studies of human nAChR a7 subunit phosphorylation by kinases PKA, PKC and Src.

Applications

Unspecified application

Species

Unspecified reactive species

Syed Ahmad,Zhe She,Heinz-Bernhard Kraatz

Nature communications 9:2590 PubMed29968724

2018

Postprandial FGF19-induced phosphorylation by Src is critical for FXR function in bile acid homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Sangwon Byun,Dong-Hyun Kim,Daniel Ryerson,Young-Chae Kim,Hao Sun,Bo Kong,Peter Yau,Grace Guo,H Eric Xu,Byron Kemper,Jongsook Kim Kemper

The Journal of biological chemistry 292:2065-2079 PubMed27994061

2016

Focal Adhesion Kinase-mediated Phosphorylation of Beclin1 Protein Suppresses Cardiomyocyte Autophagy and Initiates Hypertrophic Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaokang Cheng,Qiang Zhu,Rachel Dee,Zachary Opheim,Christopher P Mack,Douglas M Cyr,Joan M Taylor
View all publications
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