Recombinant Staphylococcus aureus Clumping factor A protein (His tag) is a Staphylococcus aureus subsp. aureus COL Fragment protein, in the 229 to 559 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
G T D I T N Q L T N V T V G I D S G T T V Y P H Q A G Y V K L N Y G F S V P N S A V K G D T F K I T V P K E L N L N G V T S T A K V P P I M A G D Q V L A N G V I D S D G N V I Y T F T D Y V N T K D D V K A T L T M P A Y I D P E N V K K T G N V T L A T G I G S T T A N K T V L V D Y E K Y G K F Y N L S I K G T I D Q I D K T N N T Y R Q T I Y V N P S G D N V I A P V L T G N L K P N T D S N A L I D Q Q N T S I K V Y K V D N A A D L S E S Y F V N P E N F E D V T N S V N I T F P N P N Q Y K V E F N T P D D Q I T T P Y I V V V N G H I D P N S K G D L A L R S T L Y G Y N S N I I W R S M S W D N E V A F N N G S G S G D G I D K P V V P E Q P D E P G E I E P I P E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cell surface-associated protein implicated in virulence. Promotes bacterial attachment exclusively to the gamma-chain of human fibrinogen. Induces formation of bacterial clumps (By similarity).
Targets
Clumping factor A
Alternative names
Clumping factor A, Fibrinogen receptor A, Fibrinogen-binding protein A, clfA, SACOL0856
Recommended products
Recombinant Staphylococcus aureus Clumping factor A protein (His tag) is a Staphylococcus aureus subsp. aureus COL Fragment protein, in the 229 to 559 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- Q5HHM8-1
- Animal free
No
- Species
Staphylococcus aureus subsp. aureus COL
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
G T D I T N Q L T N V T V G I D S G T T V Y P H Q A G Y V K L N Y G F S V P N S A V K G D T F K I T V P K E L N L N G V T S T A K V P P I M A G D Q V L A N G V I D S D G N V I Y T F T D Y V N T K D D V K A T L T M P A Y I D P E N V K K T G N V T L A T G I G S T T A N K T V L V D Y E K Y G K F Y N L S I K G T I D Q I D K T N N T Y R Q T I Y V N P S G D N V I A P V L T G N L K P N T D S N A L I D Q Q N T S I K V Y K V D N A A D L S E S Y F V N P E N F E D V T N S V N I T F P N P N Q Y K V E F N T P D D Q I T T P Y I V V V N G H I D P N S K G D L A L R S T L Y G Y N S N I I W R S M S W D N E V A F N N G S G S G D G I D K P V V P E Q P D E P G E I E P I P E
- Accession
- Protein length
Fragment
- Predicted molecular weight
52 kDa
- Amino acids
229 to 559
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Cell surface-associated protein implicated in virulence. Promotes bacterial attachment exclusively to the gamma-chain of human fibrinogen. Induces formation of bacterial clumps (By similarity).
- Sequence similarities
Belongs to the serine-aspartate repeat-containing protein (SDr) family.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Clumping factor A often referred to as ClfA is a protein expressed on the surface of Staphylococcus aureus. It functions as an adhesin which facilitates bacterial adhesion to fibrinogen in host tissues. This protein is about 82 kDa in mass and its ability to bind fibrinogen leads to bacterial clumping a critical step in infection establishment. Expressed primarily on the surface of S. aureus cells ClfA enables the bacterium to colonize host tissues by forming aggregates.
- Biological function summary
This protein plays a critical role in the initial stages of S. aureus infection. ClfA is not part of a larger protein complex; instead it functions independently to recognize and bind to fibrinogen a glycoprotein found in blood plasma. This interaction contributes to the bacterium's ability to evade the immune response facilitating persistent colonization and subsequent infection. The direct interaction between ClfA and fibrinogen is essential for S. aureus to adhere to and invade host tissues.
- Pathways
This protein is integral to the pathogenesis of Staphylococcus aureus by mediating biofilm formation and host tissue invasion. It is an important player in the bacterial surface components that recognize adhesive matrix molecules (MSCRAMM) pathway which also involves other proteins like fibronectin-binding proteins (FnBPs). ClfA's binding to fibrinogen is just one mechanism within this pathway that contributes to biofilm maturation and persistence of infection. This interaction is a tactical advantage for S. aureus allowing it to evade host defenses and establish infections effectively.
- Associated diseases and disorders
ClfA is strongly associated with conditions like infective endocarditis and septic arthritis. In these diseases ClfA's interaction with fibrinogen promotes the formation of bacterial clumps on heart valves and in joint spaces respectively. Other proteins involved in Staphylococcus aureus infection such as Spa (protein A) are critical as well as they work in conjunction with ClfA to advance the pathogen's virulence. Targeting ClfA and its molecular interactions could offer therapeutic potential for tackling S. aureus-associated diseases.
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1 product image
SDS-PAGE - Recombinant Staphylococcus aureus Clumping factor A protein (His tag) (ab225978)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analyisi of ab225978 with 5% enrichment gel and 15% separation gel.
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