JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB52953

Recombinant Staphylococcus aureus Protein A

Be the first to review this product! Submit a review

|

(3 Publications)

Recombinant Staphylococcus aureus Protein A is a Fragment protein in the 32 to 327 aa range with >99% purity, < 0.1 EU/mg endotoxin level and suitable for SDS-PAGE.The predicted molecular weight of ab52953 protein is 35.5 kDa.

- Save time and ensure accurate results - use our recombinant S. aureus Protein A (SpA) to probe and detect IgG

View Alternative Names

SAOUHSC_00069, spa, Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SpA

2 Images
Size Exclusion Chromatography - Recombinant Staphylococcus aureus Protein A (AB52953)
  • Size Exclusion Chromatography

Supplier Data

Size Exclusion Chromatography - Recombinant Staphylococcus aureus Protein A (AB52953)

SEC chromatography of Protein A. The apparent molecular weight is ~ 70 KDa calculated based on SEC standard curve.

SDS-PAGE - Recombinant Staphylococcus aureus Protein A (AB52953)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Staphylococcus aureus Protein A (AB52953)

SDS-PAGE (4-20%) of Recombinant Protein A.

Lane 1-3 : 5, 2.5, and 1 μg, Protein A under Reducing conditions,

Lane4 : protein Ladder;

Lane5-7 : 5, 2.5, and 1 μg of Protein A under Non-Reducing conditions

Key facts

Purity

>99% SDS-PAGE

Endotoxin level

< 0.1 EU/mg

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P02976

Animal free

Yes

Carrier free

No

Species

Staphylococcus aureus subsp. aureus NCTC 8325

Storage buffer

Constituents: Water

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Ensure the validity of your result using our recombinant S. aureus Protein A ab52953 to probe and detect IgG in SDS-PAGE.

Check out our protein gel staining guide for SDS-PAGE here


Function

The cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from this protein to ensure the maximum specific IgG binding. The 6x His-tag can be used for affinity purification or for Protein A detection using anti-His-tag antibody. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies.

Protein A binds to most human and mouse IgG subclasses (e.g: human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3).

It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit.
Protein A has little affinity to chicken, goat, rat and sheep.


This product is manufactured by BioVision, an Abcam company and was previously called 6500 Protein A (Liquid form). 6500-10 is the same size as the 10 mg size of ab52953.
style
left-column

Sequence info

[{"sequence":"AANAAQHDEAQQNAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNKDQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNKEQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNKEQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNKEQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPK","proteinLength":"Fragment","predictedMolecularWeight":"35.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":327,"aminoAcidStart":32,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P02976","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column
style
left-column

Specifications

Form

Liquid

Additional notes

Purity: >99% by SDS-PAGE and HPLC analyses.

style
left-column

General info

Function

Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins (PubMed : 10805799, PubMed : 2938951, PubMed : 4163007). In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).

Sequence similarities

Belongs to the immunoglobulin-binding protein SpA family.

style
left-column

Product protocols

style
left-column

Target data

Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins (PubMed : 10805799, PubMed : 2938951, PubMed : 4163007). In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).
See full target information spa
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Diagnostics (Basel, Switzerland) 13: PubMed37175021

2023

Application of a Novel One-Side Cell Quartz Crystal Microbalance Immunosensor in the Determination of Alpha-Fetoprotein from Human Serum.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Chen,Huashan Shi,Bo Mu

The Analyst 144:5108-5116 PubMed31373337

2019

Influence of antibody immobilization strategy on carbon electrode immunoarrays.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed Sharafeldin,Kira McCaffrey,James F Rusling

Small (Weinheim an der Bergstrasse, Germany) 14:e1802865 PubMed30334351

2018

Microparticle Delivery of Protein Markers for Single-Cell Western Blotting from Microwells.

Applications

Unspecified application

Species

Unspecified reactive species

John J Kim,Peggy P Y Chan,Julea Vlassakis,Alisha Geldert,Amy E Herr
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Proteins & Peptides

AB84187

Recombinant Staphylococcus aureus Protein A

SDS-PAGE - Recombinant Staphylococcus aureus Protein A (AB84187)

  • 0
  • 0

Complementary Products

Assay Kits

AB133036

Protein A ELISA Kit

Sandwich ELISA - Protein A ELISA Kit (AB133036)

  • 0
  • 0
Proteins & Peptides

AB52213

Recombinant Protein A + Protein G

SDS-PAGE - Recombinant Protein A + Protein G (AB52213)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB18596

HRP Anti-Protein A antibody

  • 0
  • 0
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com