Recombinant Staphylococcus aureus Protein A is a Staphylococcus aureus subsp. aureus NCTC 8325 Fragment protein, in the 32 to 327 aa range, expressed in Escherichia coli, with >99% purity, < 0.1 EU/mg endotoxin level and suitable for SDS-PAGE.
Images
Publications
Filter by
- Diagnostics (Basel, Switzerland) 13:2023Application of a Novel One-Side Cell Quartz Crystal Microbalance Immunosensor in the Determination of Alpha-Fetoprotein from Human Serum.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Chen,Huashan Shi,Bo MuPubMed 37175021
- The Analyst 144:5108-51162019Influence of antibody immobilization strategy on carbon electrode immunoarrays.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohamed Sharafeldin et. al.PubMed 31373337
- Small (Weinheim an der Bergstrasse, Germany) 14:e18028652018Microparticle Delivery of Protein Markers for Single-Cell Western Blotting from Microwells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohn J Kim et. al.PubMed 30334351
Key facts
- Purity
- >99% SDS-PAGE
- Endotoxin level
- < 0.1 EU/mg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins (PubMed:10805799, PubMed:2938951, PubMed:4163007). In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).
Additional Targets
Protein A
Alternative names
SAOUHSC_00069, spa, Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SpA
Recommended products
Recombinant Staphylococcus aureus Protein A is a Staphylococcus aureus subsp. aureus NCTC 8325 Fragment protein, in the 32 to 327 aa range, expressed in Escherichia coli, with >99% purity, < 0.1 EU/mg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >99% SDS-PAGE
- Endotoxin level
- < 0.1 EU/mg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P02976-1
- Animal free
- Yes
- Species
- Staphylococcus aureus subsp. aureus NCTC 8325
- Concentration
- Storage buffer
Constituents: Water
Sequence info
Amino acid sequence
- Accession
- P02976
- Protein length
- Fragment
- Predicted molecular weight
- 35.5 kDa
- Amino acids
- 32 to 327
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Purity: >99% by SDS-PAGE and HPLC analyses.
General info
- Function
Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins (PubMed:10805799, PubMed:2938951, PubMed:4163007). In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).
- Sequence similarities
Belongs to the immunoglobulin-binding protein SpA family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is manufactured by BioVision, an Abcam company and was previously called 6500 Protein A (Liquid form). 6500-10 is the same size as the 10 mg size of ab52953.
The cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from this protein to ensure the maximum specific IgG binding. . The 6x His-tag can be used for affinity purification or for protein A detection using anti-His-tag antibody. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies.
Protein A binds to most human and mouse IgG subclasses (e.g: human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3).
It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit.
Protein A has little affinity to chicken, goat, rat and sheep.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
SDS-PAGE - Recombinant Staphylococcus aureus Protein A (ab52953)
SDS-PAGE (4-20%) of Recombinant Protein A.
Lane 1-3: 5, 2.5, and 1 μg, Protein A under Reducing conditions,
Lane4: protein Ladder;
Lane5-7: 5, 2.5, and 1 μg of Protein A under Non-Reducing conditions
Size Exclusion Chromatography - Recombinant Staphylococcus aureus Protein A (ab52953)
SEC chromatography of Protein A. The apparent molecular weight is ~ 70 KDa calculated based on SEC standard curve.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com