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Recombinant Streptavidin protein is a Streptomyces avidinii Full Length protein, in the 1 to 183 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, FuncS.

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Key facts

Purity
>90% SDS-PAGE
Expression system
Escherichia coli
Tags
His-T7 tag N-Terminus
Applications
SDS-PAGE, FuncS
Biologically active
Yes

Amino acid sequence

M A S M T G G Q Q M G R G H H H H H H E N L Y F Q G G E F D P S K D S K A Q V S A A E A G I T G T W Y N Q L G S T F I V T A G A D G A L T G T Y E S A V G N A E S R Y V L T G R Y D S A P A T D G S G T A L G W T V A W K N N Y R N A H S A T T W S G Q Y V G G A E A R I N T Q W L L T S G T T E A N A W K S T L V G H D T F T K V K P S A A S I D A A K K A G V N N G N P L D A V Q Q

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

ab216251 can be used for assaying biotin conjugated targets, such as protein, small molecular and others in various detection technologies.

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Target data

Function

The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).

Alternative names

Recommended products

Recombinant Streptavidin protein is a Streptomyces avidinii Full Length protein, in the 1 to 183 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, FuncS.

Key facts

Purity
>90% SDS-PAGE
Expression system
Escherichia coli
Applications
SDS-PAGE, FuncS
Biological activity
Activity: > 17U / mg (one unit = streptavidin binds 1 μg D-biotin at pH 8.9).
Accession
P22629-1
Animal free
No
Species
Streptomyces avidinii
Concentration
Loading...
Storage buffer

pH: 8
Constituents: 0.32% Tris HCl

Sequence info

Amino acid sequence

M A S M T G G Q Q M G R G H H H H H H E N L Y F Q G G E F D P S K D S K A Q V S A A E A G I T G T W Y N Q L G S T F I V T A G A D G A L T G T Y E S A V G N A E S R Y V L T G R Y D S A P A T D G S G T A L G W T V A W K N N Y R N A H S A T T W S G Q Y V G G A E A R I N T Q W L L T S G T T E A N A W K S T L V G H D T F T K V K P S A A S I D A A K K A G V N N G N P L D A V Q Q
Accession
P22629
Protein length
Full Length
Predicted molecular weight
19.72 kDa
Amino acids
1 to 183
Nature
Recombinant
Tags
His-T7 tag N-Terminus

Specifications

Form
Liquid

General info

Function

The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).

Sequence similarities

Belongs to the avidin/streptavidin family.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

ab216251 can be used for assaying biotin conjugated targets, such as protein, small molecular and others in various detection technologies

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

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