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AB279315

Recombinant Streptavidin protein (HRP)

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Recombinant Streptavidin protein (HRP) is a Streptomyces avidinii Full Length protein, expressed in Escherichia coli, with >90%, suitable for ELISA, WB.

View Alternative Names

Streptavidin

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ELISA - Recombinant Streptavidin protein (HRP) (AB279315)
  • ELISA

Supplier Data

ELISA - Recombinant Streptavidin protein (HRP) (AB279315)

ELISA plate was coated with Goat Anti-Human IgG-Biotin and purified/unlabeled Rat IgG1κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted ab279315.

Western blot - Recombinant Streptavidin protein (HRP) (AB279315)
  • WB

Supplier Data

Western blot - Recombinant Streptavidin protein (HRP) (AB279315)

Rabbit IgG-unlabeled was resolved by electrophoresis under reducing conditions, transferred to PVDF membrane, and probed with Goat Anti-Rabbit IgG-Biotin followed by ab279315 and chemiluminescent detection.

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Western blot - Recombinant Streptavidin protein (HRP) (ab279315)

false

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

WB, ELISA

applications

Biologically active

Yes

Biological activity

The specific activity has been determined to be ≥ 12 U/mg protein (HABA dye-binding method) and ≥ 14 U/mg protein (biotin titration method).

Conjugation

HRP

Excitation/Emission
Accession

P22629

Animal free

Yes

Carrier free

No

Species

Streptomyces avidinii

Storage buffer

pH: 7.4 Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":null,"proteinLength":"Full Length","predictedMolecularWeight":"19 kDa","actualMolecularWeight":"60 kDa","aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P22629","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
True
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Specifications

Form

Liquid

Additional notes

nan

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General info

Function

The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).

Sequence similarities

Belongs to the avidin/streptavidin family.

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Product protocols

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Target data

The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
See full target information Streptavidin
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Product promise

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