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AB168007

Recombinant Streptococcus pneumoniae Penicillin Binding Protein 2X

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(1 Publication)

Recombinant Streptococcus pneumoniae Penicillin Binding Protein 2X is a Streptococcus pneumoniae R6 Fragment protein, in the 50 to 750 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

View Alternative Names

spr0304, pbpX, Penicillin-binding protein 2X, PBP-2X, PBP2X

1 Images
SDS-PAGE - Recombinant Streptococcus pneumoniae Penicillin Binding Protein 2X (AB168007)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Streptococcus pneumoniae Penicillin Binding Protein 2X (AB168007)

SDS-PAGE analysis of ab168007.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P59676

Animal free

No

Carrier free

No

Species

Streptococcus pneumoniae R6

Storage buffer

pH: 7.4 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"TGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDENYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITYANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSGMESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQMDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYEPGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGFAHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSFGQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAASLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLTDYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTAKALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAPNQQVLILSDKAEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKDIKKITLTLGD","proteinLength":"Fragment","predictedMolecularWeight":"76.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":750,"aminoAcidStart":50,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P59676","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Penicillin Binding Protein 2X (PBP2X) functions as a transpeptidase enzyme. It is key in forming cross-links between peptidoglycan chains during bacterial cell wall synthesis. PBP2X is often referred to by its abbreviation and it is a high-molecular-mass penicillin binding protein around 82 kDa. This protein is expressed mainly in the membrane of gram-positive bacteria such as Streptococcus pneumoniae. Its function makes it a target for beta-lactam antibiotics which aim to inhibit its enzymatic action.
Biological function summary

The protein plays an essential role in bacterial cell wall synthesis which is vital for maintaining cell shape and integrity. PBP2X is part of a larger complex known as the divisome a multi-protein machine involved in cell division. Within this complex PBP2X collaborates with other penicillin-binding proteins (PBPs) to catalyze the final stages of peptidoglycan synthesis. Its activity ensures proper formation and remodeling of the bacterial cell wall during growth and division.

Pathways

PBP2X is involved in the peptidoglycan biosynthesis pathway important for bacterial cell wall integrity and activity. This pathway also includes other PBPs like PBP1A and PBP2B which have overlapping roles in transpeptidase and glycosyltransferase functions. Additionally PBP2X interacts with components of the penicillin-binding protein regulatory network which regulates bacterial response to cell wall-directed antibiotics.

PBP2X is closely linked to antibiotic resistance particularly in Streptococcus pneumoniae. Mutations in PBP2X lead to changes in antibiotic binding affinity contributing to penicillin resistance in these bacterial strains. PBP2X mutations often occur alongside changes in other PBPs such as PBP1A compounding resistance and complicating treatment options. Understanding these mutations aids in the development of new antibacterial strategies and helps combat increasing rates of antibiotic-resistant infections.
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Specifications

Form

Liquid

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General info

Function

A transpeptidase that forms peptide cross-links between adjacent glycan strands in cell wall peptidoglycan (PG). Part of the divisome machinery that synthesizes the septal cross wall. Beta-lactams inactivate the PBPs by acylating an essential serine residue in the active site of these proteins.

Sequence similarities

Belongs to the transpeptidase family.

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Product protocols

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Target data

A transpeptidase that forms peptide cross-links between adjacent glycan strands in cell wall peptidoglycan (PG). Part of the divisome machinery that synthesizes the septal cross wall. Beta-lactams inactivate the PBPs by acylating an essential serine residue in the active site of these proteins.
See full target information pbpX
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular biology (Noisy-le-Grand, France) 70:40-47 PubMed38650157

2024

Identification of small molecule inhibitors of penicillin-binding protein 2a of methicillin-resistant Staphylococcus aureus for the therapeutics of bacterial infection.

Applications

Unspecified application

Species

Unspecified reactive species

Anwer Razique
View all publications
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