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AB63978

Recombinant Streptolysin O (Hemolytic streptococcus) protein

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(5 Publications)

Recombinant Streptolysin O (Hemolytic streptococcus) protein is a Streptococcus dysgalactiae subsp. equisimilis Full Length protein, in the 37 to 574 aa range, expressed in Escherichia coli, with >98%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Streptolysin O, SLO, Thiol-activated cytolysin, slo

1 Images
SDS-PAGE - Recombinant Streptolysin O (Hemolytic streptococcus) protein (AB63978)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Streptolysin O (Hemolytic streptococcus) protein (AB63978)

SDS-PAGE analysis of purified Streptolysin O (Hemolytic streptococcus) protein (ab63978).

Key facts

Purity

>98% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Definition of 1HU is activation of 50% hemolysis by incubating 3% sheep red blood cells at 37℃ for 30 minutes.

The specific activity is as high as 1,200,000- 2,000,000 hemolytic units (HU) /mg.

Accession

Q54114

Animal free

No

Carrier free

No

Species

Streptococcus dysgalactiae subsp. equisimilis

Storage buffer

pH: 6 - 8.5 Constituents: x1 Phosphate Buffered Saline, 50% Glycerol (glycerin, glycerine), 49% Water, 0.1% Streptolysin O, 0.015% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>The specific activity is as high as 700,000 to ~1,900,000 hemolytic units (HU) /mg.Definition of 1HU is the activation of 50% hemolysis on 3% sheep red blood cells.Restoration of activity can be done by 20 mM cysteine or 10 mM DTT.</p>" } } }
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Product details

Information about health hazards:

Target: Cholesterol on human and animal cell membrane

Health Hazards: May be fatal if enters bloodstream.

LD50-Lethal dose (50 percent kill) intravenous,

Rabbit, 1500 ng/kg (Ref :PHTHDT Pharmacology and Therapeutics. (Pergamon Press Ltd.,Headington Hill Hall, Oxford OX3 0BW, UK) Vol.(Issue) 11, Page 661,1981 )

Guinea pig, 12 ug/kg (Ref: BICMBE Biochimie. (SPPIF, B.P.22, F-41353 Vineuil, France, Vol.(Issue)55,Page 1187, 1973)

Toxicity is much less when introduced via other routes of entry like interdermal injection.

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Sequence info

[{"sequence":"MGGSHHHHHHGMASMTGGQQMGRDLYDDDDKDPSSRNKQNTANTETTTTNEQPKPESSELTTEKAGQKMDDMLNSNDMIKLAPKEMPLESAEKEEKKSEDNKKSEEDHTEEINDKIYSLNYNELEVLAKNGETIENFVPKEGVKKADKFIVIERKKKNINTTPVDISIIDSVTDRTYPAALQLANKGFTENKPDAVVTKRNPQKIHIDLPGMGDKATVEVNDPTYANVSTAIDNLVNQWHDNYSGGNTLPARTQYTESMVYSKSQIEAALNVNSKILDGTLGIDFKSISKGEKKVMIAAYKQIFYTVSANLPNNPADVFDKSVTLKELQRKGVSNEAPPLFVSNVAYGRTVFVKLETSSKSNDVEAAFSAALKGTDVKTNGKYSDILENSSFTAVVLGGDAAEHNKVVTKDFDVIRNVIKDNATFSRKNPAYPISYTSVFLKNNKIAGVNNRSEYVETTSTEYTSGKINLSHQGAYVAQYEILWDEINYDDKGKEVITKRRWDNNWYSKTSPFSTVIPLGANSRNIRIMARECTGLAWEWWRKVIDERDVKLSKEINVNISGSTLSPYGSITYK","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":574,"aminoAcidStart":37,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q54114","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Streptolysin O also known as Hemolytic streptococcus or SLO is a cytolytic toxin produced by some strains of group A Streptococcus. It forms pores in host cell membranes leading to cell lysis. Streptolysin O has a molecular mass of approximately 69 kDa. This protein expresses predominantly in the bacterial cell environment during infections. It is an oxygen-labile toxin which means it is inactivated by oxygen. The immune system of the host generates antibodies called anti-streptolysin O or anti-SLO to neutralize its effects.
Biological function summary

Streptolysin O disrupts cellular membranes by binding to cholesterol which results in host cell damage and death. It is not part of a multi-protein complex but interacts directly with host cell membranes. During infection this interaction causes significant damage to tissues and allows the bacteria to invade and spread rapidly. Streptolysin O contributes significantly to the pathogenicity of Streptococcus pyogenes making the bacteria highly virulent.

Pathways

Streptolysin O influences host immune response and contributes to inflammation. During infection it activates the NF-kB signaling pathway which plays a role in immune responses and inflammation. This toxin also interacts with other proteins such as leukotoxins to enhance the virulence of the bacterial infection. The pathway activation can lead to increased production of pro-inflammatory cytokines which are part of the body's defense mechanism.

Streptolysin O is implicated in diseases like strep throat and rheumatic fever. The immune response to Streptolysin O leads to the production of anti-streptolysin O antibodies which are often measured to diagnose recent streptococcal infections. Additionally the dysregulation caused by Streptolysin O in host pathways can contribute to post-infectious complications like acute glomerulonephritis with connections to other streptococcal surface proteins such as M protein which also play roles in host-pathogen interactions.
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Specifications

Form

Liquid

Additional notes

Affinity-purified with Ni colum followed by gel-filtration

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General info

Function

A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.

Sequence similarities

Belongs to the cholesterol-dependent cytolysin family.

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Product protocols

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Target data

A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.
See full target information Streptolysin O

Additional targets

Streptolysin O (Hemolytic streptococcus)
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

EBioMedicine 30:303-316 PubMed29463472

2018

Interferon Gamma Induces Reversible Metabolic Reprogramming of M1 Macrophages to Sustain Cell Viability and Pro-Inflammatory Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Feilong Wang,Song Zhang,Ryounghoon Jeon,Ivan Vuckovic,Xintong Jiang,Amir Lerman,Clifford D Folmes,Petras D Dzeja,Joerg Herrmann

Human molecular genetics 26:2747-2767 PubMed28453723

2017

GTP binding regulates cellular localization of Parkinson's disease-associated LRRK2.

Applications

Unspecified application

Species

Unspecified reactive species

Marian Blanca Ramírez,Antonio Jesús Lara Ordóñez,Elena Fdez,Jesús Madero-Pérez,Adriano Gonnelli,Matthieu Drouyer,Marie-Christine Chartier-Harlin,Jean-Marc Taymans,Luigi Bubacco,Elisa Greggio,Sabine Hilfiker

Scientific reports 6:31055 PubMed27503796

2016

Proteome mapping of Plasmodium: identification of the P. yoelii remodellome.

Applications

Unspecified application

Species

Unspecified reactive species

Anthony Siau,Ximei Huang,Mei Weng,Siu Kwan Sze,Peter R Preiser

Pflugers Archiv : European journal of physiology 466:1831-44 PubMed24327207

2013

Modeling of Friedreich ataxia-related iron overloading cardiomyopathy using patient-specific-induced pluripotent stem cells.

Applications

FuncS

Species

Unspecified reactive species

Yee-Ki Lee,Philip Wing-Lok Ho,Revital Schick,Yee-Man Lau,Wing-Hon Lai,Ting Zhou,Yanhua Li,Kwong-Man Ng,Shu-Leung Ho,Miguel Angel Esteban,Ofer Binah,Hung-Fat Tse,Chung-Wah Siu

Methods in molecular biology (Clifton, N.J.) 1022:277-82 PubMed23765668

2013

Mannose-6-phosphate: a regulator of LLO destruction.

Applications

Unspecified application

Species

Unspecified reactive species

Ningguo Gao,Mark A Lehrman
View all publications
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