Recombinant Toxin zeta protein (His tag) is a Streptococcus agalactiae Full Length protein, in the 1 to 287 aa range, expressed in Yeast, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Yeast
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M A N I T D F T E K Q F E D R L E K N V E R L T K N R L A V E S P T A F L L G G Q P G S G K T S L R S A I S E E T Q G N V V I I D N D T F K Q Q H P N F D E L V K L Y E K D V V K Y V T P Y S N R M T E A I I S R L R D K G Y N L V I E G T G R T T D V P I Q T A T M L Q A K D Y E T K M Y V M A V P K I N S Y L G T I E R Y E T M Y A D D P M T A R A T P K Q A H D I V V K N L P T N L E T L H K T G L F S D I R L Y N R E G V K L Y S S L E T P S I S P K E T L E R E L N R K V S G K E I Q P T L E R I E Q K M V Q N Q H Q E T P E F K A I Q Q K M E S L Q P P T P P I P K T P K L P G I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Toxic component of a type II toxin-antitoxin (TA) system. Phosphorylates UDP-N-acetyl-D-glucosamine (UNAG) on the 3'-hydroxyl group of the N-acetyl-D-glucosamine moiety, yielding UNAG-3P. UNAG-3P inhibits MurA, the first committed step in cell wall synthesis, which is then blocked. Phosphorylation is inhibited by cognate epsilon antitoxin. Part of a postsegregational killing (PSK) system involved in the killing of plasmid-free cells. The zeta toxin induces programmed cell death (By similarity).
Alternative names
Toxin zeta, UDP-N-acetylglucosamine kinase, UNAG kinase
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Recombinant Toxin zeta protein (His tag) is a Streptococcus agalactiae Full Length protein, in the 1 to 287 aa range, expressed in Yeast, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Yeast
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P0A4M2-1
- Animal free
- No
- Species
- Streptococcus agalactiae
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- M A N I T D F T E K Q F E D R L E K N V E R L T K N R L A V E S P T A F L L G G Q P G S G K T S L R S A I S E E T Q G N V V I I D N D T F K Q Q H P N F D E L V K L Y E K D V V K Y V T P Y S N R M T E A I I S R L R D K G Y N L V I E G T G R T T D V P I Q T A T M L Q A K D Y E T K M Y V M A V P K I N S Y L G T I E R Y E T M Y A D D P M T A R A T P K Q A H D I V V K N L P T N L E T L H K T G L F S D I R L Y N R E G V K L Y S S L E T P S I S P K E T L E R E L N R K V S G K E I Q P T L E R I E Q K M V Q N Q H Q E T P E F K A I Q Q K M E S L Q P P T P P I P K T P K L P G I
- Accession
- P0A4M2
- Protein length
- Full Length
- Predicted molecular weight
- 34.7 kDa
- Amino acids
- 1 to 287
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Toxic component of a type II toxin-antitoxin (TA) system. Phosphorylates UDP-N-acetyl-D-glucosamine (UNAG) on the 3'-hydroxyl group of the N-acetyl-D-glucosamine moiety, yielding UNAG-3P. UNAG-3P inhibits MurA, the first committed step in cell wall synthesis, which is then blocked. Phosphorylation is inhibited by cognate epsilon antitoxin. Part of a postsegregational killing (PSK) system involved in the killing of plasmid-free cells. The zeta toxin induces programmed cell death (By similarity).
- Sequence similarities
Belongs to the zeta toxin family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Toxin zeta also known as PezT is a bacterial protein with a molecular mass around 31 kDa. It acts as a toxin with functions inside bacterial cells especially in pathogens. This protein modifies cellular molecules by phosphorylating glutamate which affects the cell's metabolism. Toxin zeta usually gets expressed in bacteria as a part of a toxin-antitoxin system where it pairs with an antitoxin called epsilon. Many pathogenic bacteria including some strains of E. coli and Staphylococcus express this protein.
- Biological function summary
Toxin zeta functions by impacting bacterial cell division and growth causing reversible dormancy in cells. Toxin zeta becomes part of a toxin-antitoxin (TA) complex where it interacts closely with the antitoxin component to regulate toxic effects. This complex helps bacterial populations survive under stress like when antibiotics are present. By inhibiting peptidoglycan synthesis toxin zeta can restrain bacterial proliferation and induce a dormant state that offers survival benefits under adverse conditions.
- Pathways
The activities of toxin zeta fit prominently into bacterial stress response and survival mechanisms. It plays a role in pathways regulating cell growth arrest and programmed cell death. Toxin zeta influences these pathways through its interaction with the antitoxin and regulatory proteins like Lon protease which degrades the antitoxin and frees toxin zeta to exert its effects. Another related protein in these pathways is the MazF toxin which also contributes to bacterial stress responses through similar modes of action.
- Associated diseases and disorders
Toxin zeta is connected to chronic and persistent infections particularly those involving antibiotic-resistant bacteria. Its ability to induce dormancy complicates treatment efforts by allowing bacterial cells to evade antibiotic action. Furthermore in persistent infections caused by Staphylococcus aureus toxin zeta may alter treatment outcomes by its interaction with proteins like ClpP which also plays a role in bacterial stress responses. Understanding toxin zeta's functions is important for developing new antibacterial strategies and addressing antibiotic resistance issues in clinical settings.
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1 product image
SDS-PAGE - Recombinant Toxin zeta protein (His tag) (ab238386)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel of ab238386.
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