Recombinant vacA protein (His tag) is a Helicobacter pylori Fragment protein, in the 37 to 245 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
Amino acid sequence
T T V I I P A I V G G I A T G A A V G T V S G L L G W G L K Q A E E A N K T P D K P D K V W R I Q A G K G F N E F P N K E Y D L Y R S L L S S K I D G G W D W G N A A T H Y W V K G G Q W N K L E V D M K D A V G T Y N L S G L R N F T G G D L D V N M Q K A T L R L G Q F N G N S F T S Y K D S A D R T T R V D F N A K N I L I D N F L E I N N R V G S G A G R K A S S T V L T L Q A S E G I T S S K N A E I S L Y D G A T L N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Induces vacuolation of eukaryotic cells. Causes ulceration and gastric lesions.
Targets
vacA
Alternative names
Vacuolating cytotoxin autotransporter, vacA
Recommended products
Recombinant vacA protein (His tag) is a Helicobacter pylori Fragment protein, in the 37 to 245 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
- Accession
- Q48253-1
- Animal free
No
- Species
Helicobacter pylori
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
T T V I I P A I V G G I A T G A A V G T V S G L L G W G L K Q A E E A N K T P D K P D K V W R I Q A G K G F N E F P N K E Y D L Y R S L L S S K I D G G W D W G N A A T H Y W V K G G Q W N K L E V D M K D A V G T Y N L S G L R N F T G G D L D V N M Q K A T L R L G Q F N G N S F T S Y K D S A D R T T R V D F N A K N I L I D N F L E I N N R V G S G A G R K A S S T V L T L Q A S E G I T S S K N A E I S L Y D G A T L N
- Accession
- Protein length
Fragment
- Predicted molecular weight
26.6 kDa
- Amino acids
37 to 245
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Induces vacuolation of eukaryotic cells. Causes ulceration and gastric lesions.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
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3 product images
SDS-PAGE - Recombinant vacA protein (His tag) (ab225655)
Discontinuous SDS-PAGE(Tris-Glycine gel) (reduced) with 5% enrichment gel and 15% separation gel.
Mass Spectrometry - Recombinant vacA protein (His tag) (ab225655)
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab225655 could indicate that this peptide derived from E.coli-expressed Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) vacA.
Mass Spectrometry - Recombinant vacA protein (His tag) (ab225655)
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab225655 could indicate that this peptide derived from E.coli-expressed Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) vacA.
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