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AB225655

Recombinant vacA protein (His tag)

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Recombinant vacA protein (His tag) is a Helicobacter pylori Fragment protein, in the 37 to 245 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Vacuolating cytotoxin autotransporter, vacA

3 Images
SDS-PAGE - Recombinant vacA protein (His tag) (AB225655)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant vacA protein (His tag) (AB225655)

Discontinuous SDS-PAGE(Tris-Glycine gel) (reduced) with 5% enrichment gel and 15% separation gel.

Mass Spectrometry - Recombinant vacA protein (His tag) (AB225655)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant vacA protein (His tag) (AB225655)

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab225655 could indicate that this peptide derived from E.coli-expressed Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) vacA.

Mass Spectrometry - Recombinant vacA protein (His tag) (AB225655)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant vacA protein (His tag) (AB225655)

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab225655 could indicate that this peptide derived from E.coli-expressed Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) vacA.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q48253

Animal free

No

Carrier free

No

Species

Helicobacter pylori

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Sequence info

[{"sequence":"TTVIIPAIVGGIATGAAVGTVSGLLGWGLKQAEEANKTPDKPDKVWRIQAGKGFNEFPNKEYDLYRSLLSSKIDGGWDWGNAATHYWVKGGQWNKLEVDMKDAVGTYNLSGLRNFTGGDLDVNMQKATLRLGQFNGNSFTSYKDSADRTTRVDFNAKNILIDNFLEINNRVGSGAGRKASSTVLTLQASEGITSSKNAEISLYDGATLN","proteinLength":"Fragment","predictedMolecularWeight":"26.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":245,"aminoAcidStart":37,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q48253","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False

Specifications

Form

Liquid

General info

Function

Induces vacuolation of eukaryotic cells. Causes ulceration and gastric lesions.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Induces vacuolation of eukaryotic cells. Causes ulceration and gastric lesions.
See full target information Vacuolating cytotoxin autotransporter

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