JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB190383

Recombinant vaccinia virus B18R protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant vaccinia virus B18R protein is a Vaccinia virus WR Fragment protein, in the 20 to 351 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µL endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

VACWR200, B18R, OPG204, Soluble interferon alpha/beta receptor OPG204, B18

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µL

Expression system

Escherichia coli

Tags

T7 tag N-Terminus His tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Measured by neutralization of IFN-alpha effects on MCF7 cells in RT-PCR assay using IFN-alpha specific induced target gene index.

Accession

P25213

Animal free

No

Carrier free

Yes

Species

Vaccinia virus WR

Storage buffer

pH: 8 Constituents: 0.32% Tris HCl

storage-buffer

style
left-column

Complementary Products

Reagents, Controls & Accessories

AB325997

Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color) (AB325997)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB68428

Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB68428)

  • 5
  • 20
Primary Antibodies

AB179475

Anti-Integrin alpha V antibody [EPR16800]

KO Validated|RabMAb|Recombinant

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

  • 5
  • 10
Primary Antibodies

AB270968

Anti-Von Hippel Lindau/VHL antibody [EPR24354-128]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-Von Hippel Lindau/VHL antibody [EPR24354-128] (AB270968)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>For mRNA mediated iPS generation application, recommended recombinant B18R protein concentration in culture PluritonTM medium should be at 250 to 350 ng/ ml level. Combined 100 ng/ml bFGF with 300 ng/ml B18R recombinant protein in Stemgent Pluriton medium and mRNA kit will generate &gt;3 iPS colonies / 1X 10<sup>5</sup> Human BJ cell / well (6 well plate).</p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MASMTGGQQMGRGHHHHHHGNLYFQGGEFELHSYAIDIENEITEFFNKMRDTLPAKDSKWLNPACMFGGTMNDIAALGEPFSAKCPPIEDSLLSHRYKDYVVKWERLEKNRRRQVSNKRVKHGDLWIANYTSKFSNRRYLCTVTTKNGDCVQGIVRSHIRKPPSCIPKTYELGTHDKYGIDLYCGILYAKHYNNITWYKDNKEINIDDIKYSQTGKELIIHNPELEDSGRYDCYVHYDDVRIKNDIVVSRCKILTVIPSQDHRFKLILDPKINVTIGEPANITCTAVSTSLLIDDVLIEWENPSGWLIGFDFDVYSVLTSRGGITEATLYFENVTEEYIGNTYKCRGHNYYFEKTLTTTVVLE","proteinLength":"Fragment","predictedMolecularWeight":"22 kDa","actualMolecularWeight":null,"aminoAcidEnd":351,"aminoAcidStart":20,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P25213","tags":[{"tag":"T7","terminus":"N-Terminus"},{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The B18R protein also known as B18R interferon inhibitor is a viral protein that belongs to the family of poxvirus encoded immunomodulatory proteins. It has a molecular mass of approximately 43 kDa. The protein is expressed by the Vaccinia virus specifically within the extracellular environment. B18R interacts with the host's immune signaling pathways by binding to type I interferons which prevents them from interacting with their receptors effectively inhibiting the host's antiviral responses.
Biological function summary

B18R acts as an interferon inhibitor that neutralizes the activity of the host's type I interferon (IFN) responses to viral infection. This protein does not usually form part of a complex but functions independently in the extracellular space. By binding to the interferons it blocks their interaction with the interferon receptors on host cells which leads to a diminished antiviral state and allows viral replication to proceed without interruption. This function highlights its role in viral evasion of the host's immune defenses.

Pathways

B18R interferes with the interferon signaling pathway which is important for the establishment of an antiviral state in host cells. The protein directly impacts the JAK-STAT signaling pathway by preventing interferons from binding to their receptors blocking the downstream activation of STAT1 and STAT2 proteins. This interruption of the signaling sequence suppresses the expression of interferon-stimulated genes and impairs the innate immune response facilitating a more favorable environment for the virus.

B18R is mainly studied in the context of infections caused by poxviruses such as smallpox and monkeypox. By inhibiting the interferon response B18R allows these viruses to bypass the host's initial immune defenses contributing to the spread and severity of the infection. The protein's activity connects it to other viral inhibitors like E3L protein of the Vaccinia virus which also targets interferon pathways to subvert host immunity demonstrating a concerted viral strategy to evade host defenses.
style
left-column

Specifications

Form

Liquid

Additional notes

The final product was refolded using temperature shift inclusion body refolding technology and chromatographically purified.

style
left-column

General info

Function

Counteracts the antiviral effects of host IFN-alpha/beta and key IFN-inducible proteins involved in viral RNA degradation suxh as host OAS1 (PubMed : 28877647). Acts as a soluble IFN-alpha receptor and thus inhibits the interaction between host IFN-alpha and its receptor.

Sequence similarities

Belongs to the interleukin-1 receptor family.

style
left-column

Product protocols

style
left-column

Target data

Counteracts the antiviral effects of host IFN-alpha/beta and key IFN-inducible proteins involved in viral RNA degradation suxh as host OAS1 (PubMed : 28877647). Acts as a soluble IFN-alpha receptor and thus inhibits the interaction between host IFN-alpha and its receptor.
See full target information OPG204
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com