Recombinant VILIP1 Protein Standard (His tag) is a Human Full Length protein, expressed in Escherichia coli, with >=80% purity and suitable for SDS-PAGE, sELISA.
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Key facts
- Purity
- >=80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, sELISA
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner.
Alternative names
VISL1, VSNL1, Visinin-like protein 1, VILIP, VLP-1, Hippocalcin-like protein 3, HLP3
Recommended products
Recombinant VILIP1 Protein Standard (His tag) is a Human Full Length protein, expressed in Escherichia coli, with >=80% purity and suitable for SDS-PAGE, sELISA.
Key facts
- Purity
- >=80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, sELISA
- Biologically active
- No
- Accession
- P62760-1
- Animal free
- Yes
- Species
- Human
- Concentration
- Storage buffer
pH: 7.3 - 7.5
Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- Accession
- P62760
- Protein length
- Full Length
- Predicted molecular weight
- 24.5 kDa
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
General info
- Function
Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner.
- Sequence similarities
Belongs to the recoverin family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Visinin-like protein 1 commonly abbreviated as VILIP1 is a neuronal calcium sensor protein. It has a mass of about 22 kDa and consists of four EF-hand motifs that bind calcium ions. VILIP1 is primarily expressed in the central nervous system including regions such as the hippocampus and cerebral cortex. This protein interacts with membranes and other intracellular molecular targets in response to changes in calcium levels influencing cellular activities associated with calcium signaling.
- Biological function summary
Visinin-like protein 1 modulates various physiological processes within neurons. It regulates signal transduction pathways by mediating calcium-dependent interactions with other proteins. VILIP1 may be part of complexes with other signaling proteins that participate in neurotransmitter release and synaptic plasticity. Its ability to modulate the phosphorylation status of its binding partners can influence neuronal excitability and synaptic communication.
- Pathways
Various studies highlight how visinin-like protein 1 participates in critical signaling modules. This protein contributes to the regulation of the cAMP signaling pathway where it can affect the activation of adenylate cyclase. Moreover VILIP1 interacts with signaling pathways related to nicotinic acetylcholine receptors affecting the transmission of nerve impulses. It influences the dynamics between calcium signaling and cAMP affecting processes like learning and memory.
- Associated diseases and disorders
Visinin-like protein 1 relates significantly to neurodegenerative diseases. Research findings associate elevated VILIP1 levels with Alzheimer's disease where it can serve as a biomarker for disease progression. Furthermore it connects to Parkinson's disease given its role in neuronal calcium homeostasis a critical factor in disease pathology. In Alzheimer's disease VILIP1 levels can correlate with beta-amyloid plaque development and tau protein phosphorylation.
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2 product images
SDS-PAGE - Recombinant VILIP1 Protein Standard (His tag) (ab316535)
SDS-PAGE analysis of ab316535 under reducing conditions for 2ug protein.
Sandwich ELISA - Recombinant VILIP1 Protein Standard (His tag) (ab316535)
Sandwich ELISA with the capture antibody dilution at 2 ug/mL and detector antibody dilution at 0.5 ug/mL.
Downloads
Product protocols
- Visit the general protocols
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