Recombinant vpx protein (His tag) is a Human immunodeficiency virus type 2 (ISOLATE ROD) Full Length protein, in the 1 to 112 aa range, expressed in Mammalian, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Mammalian
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M T D P R E T V P P G N S G E E T I G E A F A W L N R T V E A I N R E A V N H L P R E L I F Q V W Q R S W R Y W H D E Q G M S E S Y T K Y R Y L C I I Q K A V Y M H V R K G C T C L G R G H G P G G W R P G P P P P P P P G L V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells. Targets specific host proteins for degradation by the 26S proteasome. Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host VPRPB/DCAF-1. This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1. In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription (By similarity).
Alternative names
Protein Vpx, Viral protein X, X ORF protein, vpx
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Recombinant vpx protein (His tag) is a Human immunodeficiency virus type 2 (ISOLATE ROD) Full Length protein, in the 1 to 112 aa range, expressed in Mammalian, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Mammalian
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P06939-1
- Animal free
- No
- Species
- Human immunodeficiency virus type 2 (ISOLATE ROD)
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS, 6% Trehalose
Sequence info
Amino acid sequence
- M T D P R E T V P P G N S G E E T I G E A F A W L N R T V E A I N R E A V N H L P R E L I F Q V W Q R S W R Y W H D E Q G M S E S Y T K Y R Y L C I I Q K A V Y M H V R K G C T C L G R G H G P G G W R P G P P P P P P P G L V
- Accession
- P06939
- Protein length
- Full Length
- Amino acids
- 1 to 112
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
nan
General info
- Function
Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells. Targets specific host proteins for degradation by the 26S proteasome. Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host VPRPB/DCAF-1. This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1. In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription (By similarity).
- Sequence similarities
Belongs to the lentivirus VPX protein family.
- Subcellular localisation
- Host nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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1 product image
SDS-PAGE - Recombinant vpx protein (His tag) (ab267924)
SDS-PAGE analysis of recombinant HIV-2 vpx protein (His tag) (ab267924)
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Product protocols
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