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Recombinant Western Equine Encephalitis Virus E3E2 protein (His tag) is a Western equine encephalitis virus Full Length protein, in the 261 to 668 aa range, expressed in Baculovirus infected Sf9, with >80% purity and suitable for SDS-PAGE, ELISA, WB.
Alternative names=Structural polyprotein, p130
- Purity
>80% SDS-PAGE
- Expression system
Baculovirus infected Sf9 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant Western Equine Encephalitis Virus E3E2 protein (His tag) is a Western equine encephalitis virus Full Length protein, in the 261 to 668 aa range, expressed in Baculovirus infected Sf9, with >80% purity and suitable for SDS-PAGE, ELISA, WB.
Alternative names=Structural polyprotein, p130
Key facts
- Purity
>80% SDS-PAGE
- Expression system
Baculovirus infected Sf9 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
- Predicted molecular weight
54 kDa
- Amino acids
261 to 668
- Accession
- P13897-1
- Protein length
Full Length
- Animal free
No
- Nature
Recombinant
- Species
Western equine encephalitis virus
- Concentration
- Storage buffer
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Specifications
- Form
Liquid
- Additional notes
Purified by FPLC
General info
- Function
Capsid protein
- Post-translational modifications
Structural polyprotein: Specific enzymatic cleavages in vivo yield mature proteins. Capsid protein is auto-cleaved during polyprotein translation, unmasking a signal peptide at the N-terminus of the precursor of E3/E2. The remaining polyprotein is then targeted to the host endoplasmic reticulum, where host signal peptidase cleaves it into pE2, 6K and E1 proteins. pE2 is further processed to mature E3 and E2 by host furin in trans-Golgi vesicle.
- Subcellular localisation
Host nucleus
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-80°C
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