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AB269139

Recombinant Y1-BFP protein (His tag)

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Recombinant Y1-BFP protein (His tag) is a Photobacterium Full Length protein, in the 1 to 200 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE.

View Alternative Names

Blue fluorescence protein, BFP, Y1-BFP

1 Images
SDS-PAGE - Recombinant Y1-BFP protein (His tag) (AB269139)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Y1-BFP protein (His tag) (AB269139)

SDS-PAGE analysis of ab269139.

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P80893

Animal free

No

Carrier free

No

Species

Photobacterium

Storage buffer

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Sequence info

[{"sequence":"MFKGNVQGVGTVENIDKGAKFQSLHGVSLLPIDADLQSHDIIFPEDILEGVTSGELIAINGVRLTVVHTDKSIVRFDINDALELTTLGQLKVGDKVNIEKSFKFGDMTGGRSLSGIVTGVADIVEFIEKENNRQIWIEAPEHLTEFLVEKKYIGVDGVYLVIDAIENNRFCINLLLETDMRWYKKGSKVNIEIPDIAGNW","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":200,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P80893","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Y1-BFP also commonly known as BFP tag or BFP protein is a Blue Fluorescent Protein. It is a modified derivative of the Green Fluorescent Protein (GFP) with a molecular mass of around 27 kDa. Researchers extensively use Y1-BFP products as a fluorescent marker in various biological studies because of its distinct blue fluorescence. Y1-BFP shows expression in a variety of organisms including bacterial systems plant cells and mammalian cells like in tissues or cell lines for imaging applications.
Biological function summary

Y1-BFP acts as an important tool for visualizing cellular and molecular processes. It often forms part of a larger protein complex when fused to the proteins of interest serving as a non-invasive way to track real-time movements and interactions within living cells. Y1-BFP or BFP products does not naturally occur in organisms but is engineered to assist in studying protein expression and localization in live cells. The BFP tag system enables its integration with other bioimaging modalities for broader uses.

Pathways

Y1-BFP serves as a powerful marker to analyze complex protein interactions and signaling pathways. In particular it fits well in pathways focusing on protein trafficking allowing scientists to better understand the intracellular transport processes. Y1-BFP can monitor protein movements through various pathways involving receptor signaling such as in the MAPK pathway often connected with proteins like ERK.

Y1-BFP's role as a fluorescent marker contributes indirectly to disease study and model analysis. It helps researchers study gene expression profiles related to cancer and neurodegenerative diseases. Researchers often use Y1-BFP to examine alterations in proteins such as tumor suppressors or those involved in Alzheimer's disease pathways. By visualizing these processes scientists can gain a deeper comprehension of disorder progression and potential therapeutic interventions.

Specifications

Form

Liquid

General info

Function

Blue fluorescence protein (BFP) that can bind 6,7-dimethyl-8-ribityllumazine, riboflavin, and 6-methyl-7-oxo-8-ribityllumazine as a bound fluorophore. Has no riboflavin-synthase activity.

Product protocols

Target data

Blue fluorescence protein (BFP) that can bind 6,7-dimethyl-8-ribityllumazine, riboflavin, and 6-methyl-7-oxo-8-ribityllumazine as a bound fluorophore. Has no riboflavin-synthase activity.
See full target information Y1-BFP

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