Recombinant Yellow fluorescent protein

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Yellow fluorescent protein (YFP) is a genetic mutant of green fluorescent protein (GFP) that is widely used in various biotechnological and molecular biology applications. YFP has a molecular mass of approximately 27 kDa and is known for emitting yellow-green fluorescence when excited with the proper wavelength of light. This protein is expressed in many cellular systems because scientists incorporate its gene into different organisms for experimental purposes. YFP often acts as a marker to study protein localization expression and interactions making it a popular tool in life sciences research.

Biological function summary

Yellow fluorescent protein serves as an essential tool for imaging and tracking molecules and cellular processes. Researchers commonly use YFP in combination with other fluorescent proteins to investigate protein-protein interactions and cellular dynamics. YFP becomes vital when coupled with other fluorescent colors to observe interactions within cellular complexes helping to understand cellular mechanisms at the molecular level. This protein's versatile application makes it invaluable for observing live-cell dynamics in real-time.

Pathways

Researchers use yellow fluorescent protein to study biological processes such as signal transduction and cellular signaling pathways. These pathways include the MAPK/ERK and PI3K/Akt pathways where fluorescent markers like YFP tag specific proteins to visualize their role and movement. YFP helps scientists examine early signaling events and how upstream activators modulate downstream responses. By tagging molecules in these pathways with YFP researchers gain insights into how proteins interact and function under various conditions.

Studies utilize yellow fluorescent protein to investigate neurodegenerative diseases and cancers. For instance scientists use YFP to track the accumulation of misfolded proteins in Alzheimer's disease models. It also assists in visualizing oncogenic processes highlighting its connection with proteins such as Tau and p53 through disease pathways. YFP-tagged proteins provide important insights into pathological mechanisms contributing to understanding disease progression and potential therapeutic targets.